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干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响
引用本文:原向阳,郭平毅,张丽光,王鑫,赵锐,郭秀,宋喜娥.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J].中国农业科学,2010,43(4):698-705.
作者姓名:原向阳  郭平毅  张丽光  王鑫  赵锐  郭秀  宋喜娥
作者单位:(山西农业大学农学院作物化学调控与化学除草实验室)
基金项目:国家引进国际先进农业科学技术948计划,山西省研究生优秀创新项目 
摘    要:【目的】为探明干旱胁迫(5d)及旱后复水条件下不同草甘膦剂量对抗草甘膦大豆(RR1)幼苗保护酶活性及脂质过氧化作用的影响。【方法】采用盆栽试验,在大豆的第三复叶期进行水分胁迫和草甘膦处理。【结果】(1)正常水分条件下,草甘膦增加了RR1的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,以及丙二醛(MDA)含量和相对电导率(EL),且随剂量的增加和处理5d内时间的延长而升高;草甘膦处理17d后,各指标均有所下降。(2)干旱条件下,较低剂量的草甘膦处理使RR1的SOD、POD、CAT活性随胁迫时间的延长而升高,0.92kg·hm-2处理的各保护酶活性随胁迫时间的延长呈先增加后降低趋势;然而,各剂量处理的MDA含量和EL均在胁迫第5天上升到最大;各指标在复水12d后均有所下降。(3)干旱条件下草甘膦处理的SOD、POD、CAT活性以及MDA含量和EL均高于正常水分条件下草甘膦处理。【结论】正常水分条件下,草甘膦对RR1幼苗造成的伤害可以经过一段时间的生长发育有所缓解;而干旱胁迫加剧了草甘膦对RR1幼苗伤害的原因是活性氧代谢失衡,保护酶系的活性发生变化,质膜过氧化程度加大,短期干旱胁迫后复水可提高细胞膜的抗干旱能力或适应胁迫的能力。

关 键 词:干旱  草甘膦  抗草甘膦大豆  保护酶  脂质过氧化
收稿时间:2009-05-18;

Glyphosate and Post-Drought Rewatering on Protective Enzyme Activities and Membrane Lipid Peroxidation in Leaves of Glyphosate-Resistant Soybean [Glycine max (L.) Merr.] Seedlings
YUAN Xiang-yang,GUO Ping-yi,ZHANG Li-guang,WANG Xin,ZHAO Rui,GUO Xiu,SONG Xi-e.Glyphosate and Post-Drought Rewatering on Protective Enzyme Activities and Membrane Lipid Peroxidation in Leaves of Glyphosate-Resistant Soybean [Glycine max (L.) Merr.] Seedlings[J].Scientia Agricultura Sinica,2010,43(4):698-705.
Authors:YUAN Xiang-yang  GUO Ping-yi  ZHANG Li-guang  WANG Xin  ZHAO Rui  GUO Xiu  SONG Xi-e
Affiliation:YUAN Xiang-yang1,GUO Ping-yi1,ZHANG Li-guang1,WANG Xin1,2,ZHAO Rui1,GUO Xiu1,SONG Xi-e1 (1Laboratory of Crop Chemical Regulation , Chemical Weed Control,Agronomy College,Shanxi Agricultural University,Taigu 030801,Shanxi,2Taiyuan Technology , Development Centre,Taiyuan 030009)
Abstract:Objective]The study was performed to explore glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidation in leaves of glyphoste-resistant soybean seedlings(RR1).Method]Through pot experiment, water stress and glyphosate treatments were conducted at three-trifoliolate leaf stage.Superoxide dismutase(SOD),peroxidase(POD), catalase(CAT)activities, and malondialdehyde(MDA)content and relative conductivity(EL)of RR1 were analyzed.Result]In normal water condition, glyphosate increased SOD, POD, and CAT activities, MDA content, and EL of RR1,and which increased with increasing of glyphosate dosage and prolonged time in the first 5 days after glyphosate treatment.Seventeen days later, these indicators declined to some extent.In drought condition, SOD, POD, and CAT activities of RR1 treated with higher glyphosate dosages increased first and then reduced, and that treated with lower glyphosate dosages increased with prolonged stress time.However, MDA content and EL of RR1 treated with each glyphosate dosage increased to the largest on the 5th day, and declined 12 days after rewatering.The SOD, POD, and CAT activities, MDA-content and EL of RRI treated with glyphosate in drought condition were higher than that in normal water condition.Conclusion]In normal water condition, the injury of RRIcaused by glyphosate could be eased through a period of growth and development.The reasons which drought stress aggravated the injury of RR1 caused by glyphosate were metabolic imbalance of reactive oxygen species, changes in protective enzyme activities and increased in the plasma membrane peroxidation.Rewatering after short-term drought stress could enhance the drought-resistant or stress-adaptive ability of the cell membrane.
Keywords:drought  glyphosate  glyphosate-resistant soybean  protective enzymes  membrane lipid peroxidation
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