| TGF-β受体mRNA在湖羊机体组织中的表达水平及其与排卵数的关系 |
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| 引用本文： | 李二林,李齐发,李美丽,徐业芬,胡冬利,祝洪生,李新福,谢新华,陈玲,谢庄.TGF-β受体mRNA在湖羊机体组织中的表达水平及其与排卵数的关系[J].中国农业科学,2009,42(9):3288-3295. |
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| 作者姓名： | 李二林 李齐发 李美丽 徐业芬 胡冬利 祝洪生 李新福 谢新华 陈玲 谢庄 |
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| 作者单位： | 1. 南京农业大学动物科技学院/动物生殖遗传与分子进化实验室,南京,210095
2. 湖羊原种场苏州市种羊场,江苏苏州,215200 |
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| 基金项目： | 国家自然科学基金，江苏省自然科学基金 |
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| 摘 要： | 【目的】通过分析TGF-β受体(TGF-βRⅠ、TGF-βRⅡ)mRNA组织表达谱及其表达水平与湖羊排卵数的关系,研究TGF-β受体对湖羊排卵数的影响。【方法】挑选16头经产母羊,其中8只产多羔的为高产组,8只产单羔作为对照组,使用氯前列醇钠法进行同期发情,发情后48～60 h内屠宰,测定排卵数;分离取高产组母羊的下丘脑、垂体等组织样进行组织表达谱分析,用实时荧光定量PCR法检测湖羊卵巢TGF-βRⅠ和TGF-βRⅡ基因mRNA表达水平,分析TGF-β受体表达水平与湖羊排卵数的关系。【结果】TGF-βRⅠ在生殖器官相对表达量极显著高于肺和肌肉(P＜0.01),TGF-βRⅡ在生殖器官相对表达量极显著高于其它组织(P＜0.01),是卵巢高表达基因;高产组湖羊卵巢组织TGF-βRⅠ与TGF-βRⅡ基因mRNA表达量分别极显著(P＜0.01)与显著(P＝0.011)高于对照组;湖羊卵巢组织TGF-βRⅠ和TGF-βRⅡ基因mRNA表达量与排卵数呈正相关,相关系数分别为0.562 (P＞0.05)和0.711(P＜0.05)。【结论】TGF-β受体在卵巢组织中表达高,其中高产组TGF-βRⅠ和TGF-βRⅡ基因mRNA表达量极显著或显著高于对照组,而且与排卵数呈正相关。
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| 关 键 词： | 湖羊" target="_blank">face="Verdana">湖羊 排卵数 TGF-βRⅠ TGF-βRⅡ 相关 |
| 收稿时间： | 2008-12-08; |
Relationship Between the mRNA Expression Level of TGF-β Receptors in Hu Sheep's Tissues and the Number of Ovulation |
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| LI Er-lin,LI Qi-fa,LI Mei-li,XU Ye-fen,HU Dong-li,ZHU Hong-sheng,LI Xin-fu,XIE Xin-hua,CHEN Ling,XIE Zhuang.Relationship Between the mRNA Expression Level of TGF-β Receptors in Hu Sheep's Tissues and the Number of Ovulation[J].Scientia Agricultura Sinica,2009,42(9):3288-3295. |
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| Authors: | LI Er-lin LI Qi-fa LI Mei-li XU Ye-fen HU Dong-li ZHU Hong-sheng LI Xin-fu XIE Xin-hua CHEN Ling XIE Zhuang |
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| Affiliation: | (Animal Reproductive Genetic and Molecular Evolutionary Laboratory, College of Animal Science and Technology, Nanjing Agriculture University)
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| Abstract: | 【Objective】 The impact of TGF-β receptors (TGF-βRⅠ,TGF-βRⅡ) on the number of ovulation was studied by analyzing the tissue expression pattern and the relationship between the mRNA expression level of TGF-β receptors and the number of ovulation. 【Method】 Sixteen multiparous ewes were selected to investigate, eight which bred multi-lamb were used as the high-yield group, the other eight which bred single lamb were used as control group. Cloprostenol sodium were injected to make the synchronization of estrus treatment, then slaughtered within 48-60 h after empathema, two side ovaries were collected after slaughtering, the number of corpus rubrum on the ovaries was counted to deternine the number of ovulation; the tissues such as hypothalamus, hypophysis were collected from one ewe in the high-yielding group, to make a tissue expression analysis. The relationship between the mRNA expression level of TGF-β receptors and the number of ovulation, which were detected by real-time fluorescent quantitative PCR, were analyzed.【Result】 TGF-βRⅠ’s relative expression level in the reproductive organ was only significantly higher than in the lung and muscle (P＜0.01). TGF-βRⅡ’s relative expression level in reproductive organ was significantly higher than that of other tissues (P＜0.01), it is a high-expressed gene in ovary. TGF-βRⅠand TGF-βRⅡ’s mRNA expression level in the ovaries of the high-yield group were significantly higher (P＜0.01) and obviously higher (P=0.011) than the control group, respectively. TGF-βRⅠand TGF-βRⅡ’s mRNA expression level have a positive correlation with the number of ovulation, the correlation coefficient were 0.562 (P＞0.05) and 0.711 (P＜0.05), respectively. 【Conclusion】 TGF-β receptors showed the high level in ovary, TGF-βRⅠand TGF-βRⅡ’s mRNA expression level in the ovaries of the high-yielding group were extremely or obviously higher than the control group, furthermore, it had a positive correlation or a significant positive correlation with the number of ovulation, so it illusted that TGF-β receptors have a close relationship with high-yielding Hu sheep. |
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| Keywords: | Hu sheep number of ovulation TGF-βRⅠ TGF-βRⅡ correlation |
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