| 中国主栽银耳配对香灰菌的系统发育和遗传多样性 |
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| 引用本文： | 温文婷,贾定洪,郭勇,孙群,杨志荣,彭卫红.中国主栽银耳配对香灰菌的系统发育和遗传多样性[J].中国农业科学,2010,43(3):552-558. |
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| 作者姓名： | 温文婷 贾定洪 郭勇 孙群 杨志荣 彭卫红 |
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| 作者单位： | （四川大学生命科学学院） |
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| 基金项目： | 国家自然科学基金，四川省财政育种工程资助项目，四川省农业科学院优秀基金 |
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| 摘 要： | 【目的】了解中国主栽银耳配对香灰菌的分类地位及遗传多样性,为香灰菌资源利用和育种提供分子生物学依据。【方法】采用18S-28S rDNA间隔区(ITS4/ITS5)的PCR扩增和内部简单重复序列多态性(ISSR)分子标记,对中国主栽银耳配对香灰菌的遗传多样性进行分析。【结果】16株供试菌株ITS序列分析表明,供试菌株与Annulohypoxylon stygium关系最为接近,同源性高达99%。从70个ISSR引物中筛选出了11个多态性好的引物,共获得62条ISSR标记。以相关系数0.7为阈值,将16株菌划为5个遗传组,菌株间遗传差异较大,遗传多样性较高。【结论】中国主栽银耳地区的香灰菌遗传资源较为丰富,这为利用香灰菌资源选育新品种提供了可能。
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| 关 键 词： | 银耳 伴生菌 遗传多样性 聚类分析 |
| 收稿时间： | 2009-05-05; |
Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk |
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| WEN Wen-ting,JIA Ding-hong,GUO Yong,SUN Qun,YANG Zhi-rong,PENG Wei-hong.Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk[J].Scientia Agricultura Sinica,2010,43(3):552-558. |
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| Authors: | WEN Wen-ting JIA Ding-hong GUO Yong SUN Qun YANG Zhi-rong PENG Wei-hong |
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| Affiliation: | WEN Wen-ting1,JIA Ding-hong2,GUO Yong2,SUN Qun1,YANG Zhi-rong1,PENG Wei-hong1,2 (1College of Life Sciences,Sichuan University,Chengdu 610064,2Soil , Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066) |
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| Abstract: | Objective The aim of this paper is to study the classification and genetic diversity of Xianghui and to provide biological information on utilization and breeding of Xianghui. Method The phylogeny of 16 strains of Xianghui, which were collected from different provinces and paired with cultivated Tremella, was determined by ITS sequences analysis. Furthermore, the genetic diversity of these strains was analyzed by inter-simple sequence repeat (ISSR). Result The ITS sequences of the 16 strains were analyzed a... |
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| Keywords: | Tremella fuciformis accompanying fungi genetic diversity cluster analysis |
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