| 日粮添加鹅源草酸青霉产果胶酶对改善肉鸡生产性能的消化生理与免疫学机制的研究 |
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| 引用本文： | 王宝维,姜晓霞,张名爱,葛文华,岳斌.日粮添加鹅源草酸青霉产果胶酶对改善肉鸡生产性能的消化生理与免疫学机制的研究[J].中国农业科学,2011,44(15):3223-3234. |
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| 作者姓名： | 王宝维 姜晓霞 张名爱 葛文华 岳斌 |
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| 作者单位： | 1.青岛农业大学优质水禽研究所;
3.青岛农业大学食品科学与工程学院 |
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| 基金项目： | 国家水禽产业技术体系专项基金 |
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| 摘 要： | 【目的】探索鹅源草酸青霉产果胶酶的作用机理，确定其添加效果与使用方法；同时，为果胶酶的推广应用提供理论依据和技术支撑。【方法】选用1日龄同批孵化、健康肉鸡240只，随机分成4个处理组。1组为对照组，饲喂正常水平的基础日粮；2组在基础日粮的基础上添加0.249%的果胶酶；3组添加0.168%纤维素酶；4组添加0.15%由果胶酶和纤维素酶按1﹕1配合而成的复合酶。【结果】果胶酶组肉鸡。显著提高对磷（P）、粗纤维（CF）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）、蛋氨酸（Met）、赖氨酸（Lys）、胱氨酸（Cys）、苏氨酸（Thr）和异亮氨酸（Ile）的利用率（P＜0.05），极显著提高粗蛋白（CP）的利用率（P＜0.01）；显著提高果胶酶组28日龄时十二指肠、胰腺的淀粉酶、脂肪酶、胰蛋白酶活性以及空肠的脂肪酶活性（P＜0.05），极显著提高胃蛋白酶活性（P＜0.01），显著提高49日龄时胰腺的淀粉酶及脂肪酶和胰蛋白酶活性、十二指肠的淀粉酶和胰蛋白酶活性和空肠的脂肪酶和胃蛋白酶活性（P＜0.05）；显著提高空肠的绒毛高度、降低肠壁厚度（P＜0.05）；果胶酶组显著降低49日龄的尿酸、尿素氮水平（P＜0.05）;显著提高的血糖水平（P＜0.05）；显著降低盲肠大肠杆菌、沙门氏菌数量（P＜0.05）；显著提高乳酸杆菌和双歧杆菌数量（P＜0.05）；显著提高49日龄时的新城疫抗体效价（P＜0.05）。【结论】日粮中添加鹅源草酸青霉产果胶酶能提高肉鸡的生产性能和养分利用率，改善肠道形态结构、消化酶活性和微生态环境。
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| 关 键 词： | 鹅源草酸青霉产果胶酶 肉鸡 生长发育 消化生理 |
| 收稿时间： | 2010-07-20 |
Effects of Adding Pectinase Produced by Penicillium Oxalicum from Goose to Diet on the Digestive Physiology and Immunological Mechanisms for Improving the Performance of Broilers |
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| WANG Bao-wei,JIANG Xiao-xia,ZHANG Ming-ai,GE Wen-hua,YUE Bin.Effects of Adding Pectinase Produced by Penicillium Oxalicum from Goose to Diet on the Digestive Physiology and Immunological Mechanisms for Improving the Performance of Broilers[J].Scientia Agricultura Sinica,2011,44(15):3223-3234. |
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| Authors: | WANG Bao-wei JIANG Xiao-xia ZHANG Ming-ai GE Wen-hua YUE Bin |
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| Affiliation: | WANG Bao-wei1,2,JIANG Xiao-xia1,ZHANG Ming-ai1,GE Wen-hua1,YUE Bin1 (1High Quality Waterfowl Research Institute,Qingdao Agricultural University,Qingdao 266109,Shandong,2College of Food Science and Engineering,Shandong) |
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| Abstract: | 【Objective】 The objective of the experiment is to study the mechanism of pectinase produced by penicillium oxalicum Currie and Thom from goose, to determine the adding effects and the usage of pectinase in broiler diet, and to provide a theoretical basis and a technical support for the application of pectinase. 【Method】 Two hundred and forty healthy 1-day-old broiler chickens under same condition were randomly divided into 4 groups. Group 1 was the control group fed normal level of basal diet The other three groups’ diets were added with, respectively, pectinase at 0.249%, cellulose at 0.168%, compound enzyme at 0.15% prepared with pectinase and cellulose in proportion of 1﹕1. 【Result】 The growth of broilers in pectinase group was significant, pectinase improved the digestibility of P, CF, NDF, ADF, Met, Lys, Cys, Thr, and Ile significantly (P＜0.05), and the digestibility of CP was improved by 17.31% remarkably (P＜0.01). On the 28th day, compared with the control group, pectinase group’s amylase, lipase, trypsin activities of duodenum and pancreas (P＜0.05), lipase activities of jejunum were significantly increased, pepsin activity was also higher than that of control group (P＜0.01). On the 49th day pectinase group’s amylase, lipase, trypsin activities of pancreas, amylase, trypsin activities of duodenum, lipase activity of jejunum and pepsin activity were significantly higher than that of the control group (P＜0.05), jejunum’s villous height was increased significantly (P＜0.05) and the thickness of intestinal wall was decreased significantly (P＜0.05). Pectinase group’s uric acid level and urea nitrogen level significantly decreased during 0-28 days and 29-49 days, blood glucose level significantly increased on 49th day (P＜0.05), the numbers of Escherichia coli and Salmonella significantly decreased (P＜0.05), the numbers of Lactobacillus and Bacillus bifidus significantly increased (P＜0.05). Serum antibody titres to NDV of 49-day-old significantly increased (P＜0.05). 【Conclusion】 The pectinase produced by penicillium oxalicum Currie and Thom from goose could increase the production of broiler performance and nutrient utilization, and improve the gut morphology, digestive enzyme activities and micro-ecological environment. |
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| Keywords: | pectinase produced by penicillium oxalicum Currie and Thom broiler growth digestive physiology |
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