| 枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定 |
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| 引用本文： | 李宝庆,鹿秀云,郭庆港,钱常娣,李社增,马平.枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定[J].中国农业科学,2010,43(17):3547-3554. |
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| 作者姓名： | 李宝庆 鹿秀云 郭庆港 钱常娣 李社增 马平 |
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| 作者单位： | (河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心); |
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| 基金项目： | 国家"863"计划重大项目，国家科技支撑计划 |
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| 摘 要： | 【目的】对枯草芽孢杆菌Bacillus subtilis菌株BAB-1产生的脂肽类物质和挥发性物质进行分离鉴定和抑菌活性研究。【方法】采用HPLC和MALDI-TOF-MS对脂肽类物质进行分离纯化和鉴定;采用GC-MS对挥发性物质进行鉴定。【结果】HPLC分析表明,菌株BAB-1产生的脂肽类物质由LP-1、LP-2、LP-3三类组分组成,其中LP-2起主要抑菌作用。MALDI-TOF-MS质谱分析表明,LP-1为surfactin(C13—C15),LP-2为fengycin A(C14—C15)和fengycin B(C14—C18),LP-3成分未能确定。GC-MS结果表明,菌株BAB-1产生的挥发性物质由多种成分组成,主要为醇类、酮类、酸类、胺类和烷烃类等。【结论】枯草芽孢杆菌菌株BAB-1能够产生脂肽类物质和挥发性物质,并且这两类物质都具有较强的抑菌活性。
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| 关 键 词： | 枯草芽孢杆菌 脂肽 挥发性物质 抑菌活性 |
| 收稿时间： | 2010-02-01; |
Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1 |
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| LI Bao-qing,LU Xiu-yun,GUO Qing-gang,QIAN Chang-di,LI She-zeng,MA Ping.Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1[J].Scientia Agricultura Sinica,2010,43(17):3547-3554. |
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| Authors: | LI Bao-qing LU Xiu-yun GUO Qing-gang QIAN Chang-di LI She-zeng MA Ping |
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| Affiliation: | (Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province)
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| Abstract: | 【Objective】 The objective of this study was to isolate and identify the lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1, and to analyze their antifungal activities. 【Method】 Lipopepetide compounds were isolated, purified and identified by using HPLC and MALDI-TOF-MS techniques, and the volatile compounds (VCs) were identified by using GC-MS method. 【Result】 Three fractions of the lipopeptides, LP-1, LP-2 and LP-3, were collected, among which LP-2 showed the major antifungal activity. According to the mass spectra analysis, LP-1 was determined to be identical to surfactin, varying in the length of the fatty acid chain moiety from C13 to C15. LP-2 consisted of fengycin A (C14—C15) and fengycin B (C14—C18), and LP-3 remained undetermined. Many different kinds of VCs were identified, such as alcohols, ketones, acids, amines, alkanes and so on. 【Conclusion】 B. subtilis strain BAB-1 can produce lipopeptides and VCs both with strong antifungal activities. |
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| Keywords: | Bacillus subtilis lipopeptide volatile compound inhibition activity |
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