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芭蕉属植物内生细菌磷脂脂肪酸(PLFA)生物标记特性研究
引用本文:蓝江林,刘波,陈璐,肖荣凤,史怀,苏明星.芭蕉属植物内生细菌磷脂脂肪酸(PLFA)生物标记特性研究[J].中国农业科学,2010,43(10):2045-2055.
作者姓名:蓝江林  刘波  陈璐  肖荣凤  史怀  苏明星
作者单位:(福建省农业科学院农业生物资源研究所);
基金项目:国家高技术研究发展计划(863计划),福建省发展和改革委员会专项,福建省科技创新平台建设-福建省农业生物药物研究与应用平台 
摘    要:【目的】分离、鉴定芭蕉属植物内生细菌,了解内生细菌的种群结构及其脂肪酸生物标记特性。【方法】采用平板分离纯化芭蕉、威廉斯蕉和野生蕉植株内生细菌,利用细菌脂肪酸鉴定技术进行鉴定。【结果】共获得内生细菌11属14种,野生蕉植株分离得到9种,威廉斯蕉植株7种,芭蕉植株3种。共检测到35个磷脂脂肪酸生物标记,这些生物标记分为4种类型,即(1)高频次分布:在14株细菌中出现10—14次,属于细菌总体类群(general)的生物标记。(2)中频次分布:在14株细菌中出现4—6次,可以用于代表细菌属类群(genus)识别生物标记。(3)低频次分布:在14株细菌中出现2—3次,可以用于指示特定细菌种间差异的生物标记。(4)微频次分布:仅在一种细菌种类出现,是细菌种(species)特征生物标记。利用磷脂脂肪酸生物标记分析内生细菌群落结构,可将14种细菌分为3类;运用多样性、丰富度、均匀度及优势度指数等生态学概念,分析了磷脂脂肪酸生物标记总量与各生态学参数之间的关系,结果表明,磷脂脂肪酸生物标记16:0、18:1W7C、14:0、12:0、18:0、17:0CYCLO的丰富度皆相对较高;磷脂脂肪酸生物标记的Response值总和较高,均匀度(J)较低,其Simpson优势度指数(D)、Shannon-Wiener(H1)、Brillouin(H2)、Mcintosh(H3)多样性指数也相对较高。【结论】芭蕉属植物内生细菌及其磷脂脂肪酸生物标记存在多样性。

关 键 词:芭蕉属植物  内生细菌  磷脂脂肪酸生物标记
收稿时间:2009-11-11;

Characteristics of PLFA Biomarkers for the Endophytic Bacteria Inside Musa Plants
LAN Jiang-lin,LIU Bo,CHEN Lu,XIAO Rong-feng,SHI Huai,SU Ming-xing.Characteristics of PLFA Biomarkers for the Endophytic Bacteria Inside Musa Plants[J].Scientia Agricultura Sinica,2010,43(10):2045-2055.
Authors:LAN Jiang-lin  LIU Bo  CHEN Lu  XIAO Rong-feng  SHI Huai  SU Ming-xing
Affiliation:LAN Jiang-lin,LIU Bo,CHEN Lu,XIAO Rong-feng,SHI Huai,SU Ming-xing (Agricultural Bioresource Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003)
Abstract:Objective] The present paper dealt with isolation and identification of the endophytic bacteria from Musa plants as well as the characteristics of their PLFA biomarkers. Method] Healthy Musa plants were collected from Zhangzhou areas in Fujian province, China. The endophytic bacteria inside the plants were isolated by streak plate method and identified by using fatty-acid identification technology. Result] The results showed that 14 species ofendophytic bacteria were identified by means of microbe identification system. 9 microbial species belonged to Musa itinerans, 7 species belonged to Musa AAA cavendish vat.Williams and 3 species belonged to Musa basjoo. There were 35 PLFA biomarkers detected in 14 species of endophytic bacteria, in which based on the distribution frequency in 4 types of bacteria were clustered, e.g. (1) high frequency related to characteristics of microbe in general and the PFLA were detected in 10-14 times in 14 strains of bacteria, (2) middle frequency related to bacteria in general and the PFLA were detected in 4-6 times in 14 strains of bacteria, (3) low frequency related bacterial genus and the PFLA were detected in 2-3 times in 14 swains of bacteria, (4) tiny frequency related to species and the PFLA only in a bacteria. According to the PLFA biomarkers and the cluster analysis method, the 14 species were classified into 3 groups based on the PLFA biomarkers. The diversity and richness, uniformity and dominance of the concept index ecology were used to analyze the relationship between the fatty acid biomarker gross and ecological parameters. The results showed that the richness of 16:0,18:1 W7C, 14:0, 12:0, 18:0, and 17:0 CYCLO of PLFA biomarkers was relatively high. The higher fatty acids biological response value, the lower the sum uniformity (J), the dominance of the Simpson index (D), Shannon-Wiener (H1), Brillonin (H2), Mcintosh (H3) diversity index were relatively high. Conclusion] There are significant polymorphism among community structure of endophytic bacteria and their PLFA biomarkers in Musa plants.
Keywords:Musa plants  endophytic bacteria  PLFA biomarkers
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