| 鸭茅杂交种SRAP分子标记鉴定及遗传分析 |
| |
| 引用本文： | 谢文刚,张宝艺,张新全,刘伟,陈永霞,周禾.鸭茅杂交种SRAP分子标记鉴定及遗传分析[J].中国农业科学,2010,43(16):3288-3295. |
| |
| 作者姓名： | 谢文刚 张宝艺 张新全 刘伟 陈永霞 周禾 |
| |
| 作者单位： | (四川农业大学草业科学系); |
| |
| 基金项目： | 国家"十一五"科技支撑计划项目，国家自然科学基金项目 |
| |
| 摘 要： | 【目的】利用分子标记技术对鸭茅杂交种进行快速鉴定和遗传分析,建立鸭茅杂交种的快速鉴定体系,揭示鸭茅杂交种群体遗传信息,为鸭茅资源保护利用及新品种选育提供科学依据。【方法】利用SRAP标记技术对140个鸭茅杂交种单株及其亲本(01996、YA02-103)DNA进行扩增,分析杂交种与其亲本间扩增谱带的多态性,以鉴别真假杂交种,并结合形态标记对杂交后代的遗传变异进行分析。【结果】从192对SRAP引物中筛选出64对引物在杂交种和双亲间存在扩增多态性,多态性百分比为33.33%。利用能扩增出父本特征带的引物鉴定了140个杂交种,其中106个具有父本的特征带,可鉴定为真杂交种。选择16对多态性引物对亲本和106个真杂交种进行扩增,获得151条条带,其中多态性条带71条,平均每对引物扩增出4条多态性条带,多态性位点百分比为47.24%。【结论】SRAP用于鸭茅杂交种鉴定及多态性分析是可行的。
|
| 关 键 词： | 鸭茅 SRAP标记 杂交种鉴定 遗传多样性分析 |
| 收稿时间： | 2010-03-03; |
Identification and Genetic Analysis of Orchardgrass (Dactylis glomerata L.) Hybrids by SRAP Molecular Markers |
| |
| XIE Wen-gang,ZHANG Bao-yi,ZHANG Xin-quan,LIU Wei,CHEN Yong-xia,ZHOU He.Identification and Genetic Analysis of Orchardgrass (Dactylis glomerata L.) Hybrids by SRAP Molecular Markers[J].Scientia Agricultura Sinica,2010,43(16):3288-3295. |
| |
| Authors: | XIE Wen-gang ZHANG Bao-yi ZHANG Xin-quan LIU Wei CHEN Yong-xia ZHOU He |
| |
| Affiliation: | XIE Wen-gang1,ZHANG Bao-yi1,ZHANG Xin-quan1,LIU Wei1,CHEN Yong-xia1,2,ZHOU He3 (1Department of Grassland,Sichuan Agricultural University,Ya'an 625014,Sichuan,2Department of Animal Science,Xichang College,Xichang 615000,3College of Animal Science and Technology,China Agricultural University,Beijing 100193) |
| |
| Abstract: | 【Objective】 Molecular markers were used to identify orchardgrass hybrids and analyze genetic diversity for forming orchardgrass hybrid identification system, detecting genetic information and conservation and utilization of orchardgrass germplasm resources. 【Method】 The sequence-related amplified polymorphism (SRAP) were assessed to analyze 140 orchardgrass hybrid individuals and their parental lines (01996, YA02-103) for detecting true or false hybrids according to polymorphism, and the genetic variation among hybrid individuals were detected using molecular markers and morphological markers. 【Result】 Sixty-four pairs of primers that generated clear, reproducible and polymorphic bands were selected from 192 pairs of SRAP primers with an average of 33.33%. 106 progenies with the special male bands compared with the female bands were identified from 140 progenies, which were therefore recognized as true hybrids. Sixteen primer pairs produced a total of 151 bands, of which 71 bands were polymorphic bands and the average polymorphic bands was four per primer pair. The average percentage of polymorphic bands was 47.24%. 【Conclusion】 These results suggested that SRAP technique has wide prospective in hybrid identification and diversity analysis of orchardgrass. |
| |
| Keywords: | orchardgrass (Dactylis glomerata L ) SRAP markers hybrid identification genetic diversity analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
|
