| 玉米种质资源抗腐霉茎腐病和镰孢茎腐病精准鉴定 |
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| 引用本文： | 段灿星,曹言勇,董怀玉,夏玉生,李红,胡清玉,杨知还,王晓鸣.玉米种质资源抗腐霉茎腐病和镰孢茎腐病精准鉴定[J].中国农业科学,2022,55(2):265-279. |
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| 作者姓名： | 段灿星 曹言勇 董怀玉 夏玉生 李红 胡清玉 杨知还 王晓鸣 |
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| 作者单位： | 1中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京 1000812河南省农业科学院粮食作物研究所,郑州 4500023辽宁省农业科学院植物保护研究所,沈阳 1101614吉林省农业科学院植物保护研究所,吉林公主岭 136100 |
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| 基金项目： | 国家重点研发计划(2016YFD0100103);中国农业科学院农业科技创新工程(CAAS-ASTIP-2017-ICS);玉米藏粮于技(CAAS-ZDRW2020004) |
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| 摘 要： | 目的]建立和完善玉米种质抗腐霉茎腐病和镰孢茎腐病精准鉴定的方法,对遗传背景丰富的玉米种质资源进行腐霉茎腐病和镰孢茎腐病的抗性精准鉴定,筛选出对2种茎腐病具有稳定抗性的种质资源,为玉米抗病育种奠定基础.方法]对2004份具有广泛遗传背景的玉米种质进行多年多点的自然发病抗性鉴定,初步筛选出抗茎腐病或综合性状优良的代表性...
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| 关 键 词： | 玉米种质 腐霉茎腐病 镰孢茎腐病 茎基部注射接种 自然发病鉴定 精准鉴定 |
| 收稿时间： | 2021-07-12 |
Precise Characterization of Maize Germplasm for Resistance to Pythium Stalk Rot and Gibberella Stalk Rot |
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| DUAN CanXing,CAO YanYong,DONG HuaiYu,XIA YuSheng,LI Hong,HU QingYu,YANG ZhiHuan,WANG XiaoMing.Precise Characterization of Maize Germplasm for Resistance to Pythium Stalk Rot and Gibberella Stalk Rot[J].Scientia Agricultura Sinica,2022,55(2):265-279. |
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| Authors: | DUAN CanXing CAO YanYong DONG HuaiYu XIA YuSheng LI Hong HU QingYu YANG ZhiHuan WANG XiaoMing |
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| Affiliation: | 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 1000812Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou 4500023Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 1101614Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin |
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| Abstract: | 【Objective】The objective of this study is to establish and perfect the method for precise characterization of maize germplasm resistance to Pythium stalk rot (PSR) and Gibberella stalk rot (GSR), and then precisely evaluate maize accessions with rich genetic background for resistance to stalk rot, and to screen out resistant germplasm and provide valuable resources to the maize community for enhancing disease resistance. 【Method】The total of 2 004 maize accessions with extensive genetic background were identified for resistance to stalk rot in multi-plot demonstration for three years under natural condition in order to preliminarily screen out germplasm with resistance or other excellent traits. Using drilling inoculation, precise evaluation of 690 maize germplasm for resistance to PSR and GSR was conducted at 6 different sites from 2018 to 2020. 【Result】During 2016-2018, 508 accessions with different resistance to stalk rot were identified at multiple sites in non-inoculated fields, including 79, 106, and 323 highly resistant, resistant, and moderately resistant germplasm, respectively. The method of drilling inoculation at the base of stem for precise characterization of maize resistance to stalk rot was established and perfected. Correlation coefficient (r) between soil inoculation and drilling inoculation for screening of maize resistance to PSR was 0.87, with 0.84 for GSR, indicating strong correlation between maize resistance to stalk rot identified by drilling inoculation and soil inoculation. Compared with soil inoculation, the severity of stalk rot on maize treated with drilling inoculation is higher. The precise resistance evaluation of 690 maize accessions to PSR was conducted with drilling inoculation at 6 different environments in Changping of Beijing, Changge of Henan, and Yuanyang of Henan from 2018 to 2020. The incidence of PSR was considerable and the majority of accessions were susceptible or highly susceptible, merely 3.5% of maize exhibited stable PSR resistance. Correlation coefficient of maize resistance to PSR between any two sites among six different environments varied from 0.46 to 0.72. The r values between two sites in 2018, 2019, and 2020 were 0.66, 0.60, and 0.65, respectively. The r values between pairwise annual comprehensive resistance to PSR among 3 years were 0.67, 0.84, and 0.87, indicating the resistance reaction of 690 maize accessions to PSR exhibited good consistency among different environments. A total of 24 maize germplasm expressed stable resistance to PSR, such as Jizi H676, Liao 2235, Jizi 14L88, Jizi 14L101, Dan 337, M02N-23, Y1747, HRB16232, and T628358, and so on. During 2018-2020, 690 maize resources were precisely identified for resistance to GSR with drilling inoculation at 6 different environments in Shenyang of Liaoning, Lishu of Jilin, and Gongzhuling of Jilin. The results indicated GSR on maize accessions in Shenyang was severe, with the majority of maize expressing susceptibility or high susceptibility, while it was significantly slight in Lishu and Gongzhuling. Correlation coefficient of GSR resistance in 690 maize germplasm between any two sites among six environments ranged from 0.00 to 0.76. The r values between two sites in 2018, 2019, and 2020 were 0.12, 0.04, and 0.05, respectively, indicating considerable differences in GSR resistance at two sites. The r values between pairwise annual comprehensive resistance to GSR among 3 years were 0.40, 0.74, and 0.72, showing annual comprehensive GSR resistance in 690 maize accessions were relatively consistent. The six-point experimental data showed that 5 accessions (Jizi C32, Liao 785, Liao 2235, Jizi 1034, and 16SD088) exhibited stable resistance to GSR. The r value of 0.44 between PSR and GSR resistance in 690 maize germplasm indicated moderate correlation between two stalk rot resistances. 【Conclusion】The resistance level of 2004 maize accessions to stalk rot was determined at multiple environments under natural conditions. The method of drilling inoculation at the base of stem for precise characterization of maize germplasm resistance to stalk rot was established and validated. The precise resistance characterization of 690 maize accessions to PSR and GSR was performed with drilling inoculation at 6 different environments among 3 years. Twenty-four and five maize germplasm expressed stable resistance to PSR and GSR at diverse sites among 3 years, respectively, which were important sources for breeding stalk rot resistant varieties or improvement of cultivar resistance. |
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| Keywords: | maize germplasm Pythium stalk rot Gibberella stalk rot drilling inoculation non-inoculated resistance identification precise characterization |
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