| 龙眼果肉水溶性提取物对正常小鼠免疫调节作用的影响 |
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| 引用本文： | 苏东晓,张名位,廖森泰,侯方丽,张瑞芬,唐小俊,魏振承,张雁,池建伟,邓元媛.龙眼果肉水溶性提取物对正常小鼠免疫调节作用的影响[J].中国农业科学,2010,43(9):1919-1925. |
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| 作者姓名： | 苏东晓 张名位 廖森泰 侯方丽 张瑞芬 唐小俊 魏振承 张雁 池建伟 邓元媛 |
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| 作者单位： | （广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室）; |
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| 基金项目： | NSFC-广东联合基金，广东省自然科学基金团队项目 |
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| 摘 要： | 目的从体液免疫、细胞免疫和NK细胞活性等不同角度研究龙眼水溶性提取物对正常小鼠免疫调节作用的影响。方法以120只雄性昆明种小鼠为研究对象,按体重随机分5组,阴性对照组、龙眼水溶性提取物低、高剂量组(130mg·d-1、380mg·d-1)和龙眼果肉低、高剂量组(150mg·d-1、450mg·d-1),每组24只,连续35d。测定淋巴器官相对质量、脾淋巴细胞转化值、血清溶血素值、NK细胞活性和脾脏抗体生成细胞数等免疫学指标以及血清丙二醛水平、总抗氧化能力、谷胱甘肽过氧化物酶和超氧化物歧化酶活力等抗氧化指标。结果与阴性对照组比较,龙眼水溶性提取物低、高剂量组血清溶血素值和脾淋巴细胞转化值显著升高(P0.05),而龙眼果肉各剂量组效果均不显著(P0.05);高剂量龙眼水溶性提取物NK细胞活性显著增强(P0.05),但龙眼果肉剂量组无显著效果(P0.05)。龙眼果肉高剂量组血清丙二醛水平显著降低(P0.05),龙眼水溶性提取物各剂量组则差异不显著(P0.05);龙眼果肉高剂量组和龙眼水溶性提取物高剂量组血清总抗氧化能力分别极显著(P0.01)和显著(P0.05)提高。结论一定剂量的龙眼水溶性提取物能显著提高正常小鼠的体液免疫和细胞免疫能力及抗氧化活性,但抗氧化活性非其免疫调节作用的主要机制。
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| 关 键 词： | 龙眼 水溶性提取物 正常小鼠 免疫调节 抗氧化 |
| 收稿时间： | 2009-09-17; |
Effects of Water Soluble Extracts from Longan on Immune Regulation in Normal Mice |
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| SU Dong-xiao,ZHANG Ming-wei,LIAO Sen-tai,HOU Fang-li,ZHANG Rui-fen,TANG Xiao-jun,WEI Zhen-cheng,ZHANG Yan,CHI Jian-wei,DENG Yuan-yuan.Effects of Water Soluble Extracts from Longan on Immune Regulation in Normal Mice[J].Scientia Agricultura Sinica,2010,43(9):1919-1925. |
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| Authors: | SU Dong-xiao ZHANG Ming-wei LIAO Sen-tai HOU Fang-li ZHANG Rui-fen TANG Xiao-jun WEI Zhen-cheng ZHANG Yan CHI Jian-wei DENG Yuan-yuan |
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| Affiliation: | SU Dong-xiao1,2,ZHANG Ming-wei1,LIAO Sen-tai1,HOU Fang-li1,ZHANG Rui-fen1,TANG Xiao-jun1,WEI Zhen-cheng1,ZHANG Yan1,CHI Jian-wei1,DENG Yuan-yuan1 (1Key Laboratory of Functional Food,Ministry of Agriculture/Bio-Tech Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,2Food Science , Technology College,Huazhong Agricultural University,Wuhan 430070) |
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| Abstract: | 【Objective】 The objective of this experiment is to study the effects of water soluble extracts from longan on immune regulation in normal mice. 【Method】 One hundred and twenty mice were randomly divided into five groups: the normal control group, low dose longan extracts (130 mg•d-1), high dose longan extracts (380 mg•d-1), low dose longan slurry (150 mg•d-1), and high dose longan slurry (450 mg•d-1). After mice were orally administered for 35 days, changes on weight, index of immune organs, indexes of spleen lymphocyte transformation, hemolysin value of blood serum, NK cell activity, plaque-forming cell response were assayed by dissolving CRBC. Besides, four anti- oxidation indicators serum malondialdehyde level, total antioxidant capacity, glutathione peroxidase and superoxide dismutase activity were also investigated. 【Result】 Compared to normal control group, both doses of longan extracts greatly improved hemolysin value of blood serum and indexes of spleen lymphocyte transformation(P＜0.05) ,but the results showed no significant effect in the cases of longan slurry groups (P＞0.05). And high dose longan extracts enhanced NK cell activity signifcantly (P＜0.05), yet both doses of longan slurry groups showed on significant effects (P＞0.05). High dose longan slurry reduced the MDA of blood serum (P＜0.05), yet the longan extract groups did not show significant effects (P＞0.05). T-AOC was increased by high dose longan slurry (P＜0.01) and high dose longan extracts (P＜0.05), respectively. 【Conclusion】It is concluded that longan extracts have a promoting effect on immune functions and antioxidation activity of normal mice, yet the anti- oxidation activity of longan is not the main mechanism for immune regulation actions. |
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| Keywords: | longan water soluble extracts normal mice immune regulation anti-oxidation |
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