| 粳稻株高QTL与赤霉素和油菜素内酯合成及信号转导基因相关分析 |
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| 引用本文： | 姜树坤,黄成,徐正进,陈温福.粳稻株高QTL与赤霉素和油菜素内酯合成及信号转导基因相关分析[J].中国农业科学,2010,43(14):2829-2838. |
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| 作者姓名： | 姜树坤 黄成 徐正进 陈温福 |
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| 作者单位： | (沈阳农业大学农学院/农业部作物生理生态遗传育种重点开放实验室); |
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| 基金项目： | 国家自然科学基金，国家"973"重点基础研究发展计划项目 |
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| 摘 要： | 【目的】通过比较控制株高及其构成因素的QTL与赤霉素和油菜素内酯合成及信号转导相关基因的关系,为明确株高的分子调控机制提供理论参考。【方法】利用沈农265和丽江新团黑谷杂交构建的粳-粳交重组自交系为作图群体,对水稻株高及其构成因素进行QTL定位,并与控制赤霉素和油菜素内酯合成及信号转导的相关基因进行比对分析。【结果】RILs群体的株高及各个构成因素均呈正态分布。株高与各构成因素间呈正相关。相邻的构成因素间呈极显著的正相关,而相距较远的构成因素间的相关性减弱甚至负相关,进一步分析表明,株高主要受倒1和倒4节间长度的影响。共检测到21个控制株高及其构成因素的QTL,分布在第1、2、3、5、6、7、8、9、11和12染色体上。其中位于第9染色体上的QPH9b即直立穗基因EP1(DEP1或qPE9-1)对于水稻的株高起着很重要的作用,其主要通过影响倒1和倒2节间的长度来影响株高,该基因的分子功能与之前发现的众多控制株高的基因均不同,可能是一个种新的调控株高的机制。【结论】通过比较21个QTL与赤霉素和油菜素内酯合成及其信号转导相关基因发现,该群体株高的遗传基础复杂。
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| 关 键 词： | 粳稻 株高 节间长 数量性状基因座 赤霉素 油菜素内酯 |
| 收稿时间： | 2010-02-01; |
Relationship Between QTLs for Plant Height and Its Components and Genes Controlling Gibberellin and Brassinosteroid Biosynthesis and Their Transduction in Japonica Rice (Oryza sativa L.) |
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| JIANG Shu-kun,HUANG Cheng,XU Zheng-jin,CHEN Wen-fu.Relationship Between QTLs for Plant Height and Its Components and Genes Controlling Gibberellin and Brassinosteroid Biosynthesis and Their Transduction in Japonica Rice (Oryza sativa L.)[J].Scientia Agricultura Sinica,2010,43(14):2829-2838. |
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| Authors: | JIANG Shu-kun HUANG Cheng XU Zheng-jin CHEN Wen-fu |
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| Affiliation: | JIANG Shu-kun,HUANG Cheng,XU Zheng-jin,CHEN Wen-fu(College of Agronomy,Shenyang Agricultural University/Key Laboratory of Crop Physiology,Ecology,Genetics and Breeding,Ministry of Agriculture,Shenyang 110161) |
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| Abstract: | 【Objective】 To understand and provide a theoretical basis for the molecular mechanism of plant height in rice, QTLs controlling plant height and its component factors were detected and compared with the genes involved in gibberellin and brassinosteroid biosynthesis and transduction. 【Method】 The QTLs affecting plant height and its component factors were analyzed by employing 126 recombinant inbred lines (RILs) derived from a cross between two japonica rice cultivars, Shennong 265 and Lijiangxintuanheigu, and then compared them with the genes involved in gibberellin and brassinosteroid biosynthesis and transduction. 【Result】 Plant height and its component factors showed a continuous normal distribution in the RILs population. The plant height showed a high positive correlation with its component factors. The correlation between adjacent plant height components was positively significant while the significance of the correlation between non-adjacent plant height components was less or even negative. Further result indicated that the plant height was mainly affected by the length of I internode and IV internode from top. A total of 21 QTLs controlling plant height and its components were identified on chromosomes 1, 2, 3, 5, 6, 7, 8, 9, 11 and 12, respectively. QPH9b (EP1, DEP1 or qPE9-1) on chromosome 9 plays a very important role in affecting plant height through controlling the I internode and II internode length from top. Its molecular function was different from the other genes controlling plant height identified previously. So, it would provide a novel mechanism for plant height. 【Conclusion】 Comparison between 21 QTLs and genes controlling gibberellin and brassinosteroid biosynthesis and transduction indicated that the genetic basis of plant height is extremely complex in this RILs population. |
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| Keywords: | japanica rice plant height internode length quantitative trait locus gibberellin brassinosteroid |
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