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Paenibacillus polymyxa CP7菌抗革兰氏阴性菌活性组分的分离和结构分析
引用本文:陈海英,廖富蘋,林健荣.Paenibacillus polymyxa CP7菌抗革兰氏阴性菌活性组分的分离和结构分析[J].中国农业科学,2009,42(6):2105-2110.
作者姓名:陈海英  廖富蘋  林健荣
作者单位:1. 华南农业大学动物科学学院,广州,510642;温州市农业科学研究院,浙江温州,325006
2. 温州市农业科学研究院,浙江温州,325006
3. 华南农业大学动物科学学院,广州,510642
摘    要: 【目的】分离和纯化Paenibacillus polymyxa CP7菌抗革兰氏阴性菌的活性组分,并鉴定其结构。【方法】采用固相萃取柱分离、阳离子交换层析、凝胶过滤层析和HPLC层析等技术对活性组分进行分离纯化,通过紫外-可见光谱、圆二色光谱、红外光谱和MALDI-TOF-TOF-MS质谱对所分离组分进行结构鉴定。【结果】从CP7菌发酵液中分离获得抗革兰氏阴性菌的B、C1和C2组分,分子量分别为1 155、1 169和1 191 D。并确定了C1组分的分子式为C53H100O13N16,结构由6个L-α,β-二氨基丁酸(DAB)、2个苏氨酸和2个亮氨酸组成,与多粘菌素E1的结构相同。【结论】从CP7菌发酵液中分离获得了3种抗革兰氏阴性菌的小分子多肽。经测试确认C1组分为多粘菌素类E1。推测B组分也是多粘菌素类的1个成员,而C2组分则可能是多粘菌素家族中的一个新成员。

关 键 词:CP7菌  多粘类芽孢杆菌  抗菌物质  分离纯化  结构分析
收稿时间:2008-7-4

Isolation and Structure Analysis of the Components of Anti-Gram-Negative Bacteria by the Strain CP7 of Paenibacillus polymyxa
CHEN Hai-ying,LIAO Fu-ping,LIN Jian-rong.Isolation and Structure Analysis of the Components of Anti-Gram-Negative Bacteria by the Strain CP7 of Paenibacillus polymyxa[J].Scientia Agricultura Sinica,2009,42(6):2105-2110.
Authors:CHEN Hai-ying  LIAO Fu-ping  LIN Jian-rong
Affiliation:(College of Animal Science, South China Agricultural University)

Abstract:【Objective】 The components of anti-gram-negative bacteria by the strain CP7 of Paenibacillus polymyxa were purified, and the structure was identified. 【Method】 The antibacterial components from CP7 strain were purified by the solid phase extraction, cation-exchange chromatography, gel-filtration chromatography and HPLC. The components were analyzed with the technique of the ultraviolet-visible spectrum, circular dichroism spectroscopy, IR and MALDI-TOF-TOF-MS. 【Result】Three components of anti-gram-negative bacteria, B, C1 and C2, were purified from Paenibacillus polymyxa CP7 broth. Their molecular weight was 1 155 D, 1 169 D and 1 191 D, respectively. The molecular formula of C1 was C53H100O13N16, which was composed by six DAB, two threonine and two leucine, with the same structure as polymyxin E1. 【Conclusion】Three kinds of small molecule peptide of anti-gram-negative bacteria were isolated from the CP7 fermentation broth. It is speculated that B was also a member of the polymyxin family, and C2 may be a new family member.
Keywords:strain CP7  Paenibacillus polymyxa  polymyxin  isolation and purification  structure analysis
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