| 大兴安岭地区野生黑木耳菌株SRAP的遗传多样性分析 |
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| 引用本文： | 刘华晶,许修宏,姜廷波.大兴安岭地区野生黑木耳菌株SRAP的遗传多样性分析[J].中国农业科学,2011,44(13):2641-2649. |
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| 作者姓名： | 刘华晶 许修宏 姜廷波 |
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| 作者单位： | 东北农业大学资源与环境学院 |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2008BADA1B01,2008BAD95B06-03); 东北农业大学资源与环境学院青年基金(ZHQJ2009-14)、东北农业大学创新团队发展计划(CXT003-2-1) |
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| 摘 要： | 【目的】对大兴安岭地区野生黑木耳菌株进行遗传多样性分析。【方法】利用PCR-SRAP体系，选出9对SRAP引物对18个野生菌株和6个栽培菌株DNA进行扩增，通过NTSYSpc软件对遗传多样性进行分析。【结果】通过PCR扩增，9对引物共扩增到90条条带，其中78条多态性条带，多态性比率为87.0%。平均每条引物扩增的条带数和多态性条带数分别为10.00条和8.67条。多态性信息含量(PIC)变化在0.051—0.918，平均为0.683，所揭示的基因型数平均为15.78个。聚类分析结果表明，遗传相似系数在0.63水平上，可分为5个类群。【结论】SRAP标记技术在栽培与野生菌株间都表现出明显的遗传差异性，此技术可用于遗传多样性的分析研究。
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| 关 键 词： | 黑木耳 SRAP 遗传多样性 |
| 收稿时间： | 2010-08-23; |
Genetic Diversity Analysis of Wild Auricularia auricula Strains in Daxing' anling Area Using SRAP Markers |
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| LIU Hua-jing,XU Xiu-hong,JIANG Ting-bo.Genetic Diversity Analysis of Wild Auricularia auricula Strains in Daxing' anling Area Using SRAP Markers[J].Scientia Agricultura Sinica,2011,44(13):2641-2649. |
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| Authors: | LIU Hua-jing XU Xiu-hong JIANG Ting-bo |
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| Affiliation: | LIU Hua-jing1,2,XU Xiu-hong1,JIANG Ting-bo2 (1College of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,2 College of Forestry,Northeast Forestry University,Harbin 150040) |
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| Abstract: | 【Objective】Genetic diversity of wild Auricularia auricula strains in Daxing’anling area was analyzed. 【Method】Using PCR-SRAP system, 9 pairs of primers on 18 wild strains and 6 cultivated ones were selected in expansion, and through clustering method its genetic diversity was analyzed.【Result】By PCR amplification, 90 fragments were amplified. Polymorphic bands were 78 which accounted for 87.0% in the total amplified fragments. The number of amplified and polymorphic fragments was 10.00 and 8.67 per primer pair, respectively. The PIC (polymorphism information content) value of these markers varied from 0.051 to 0.918, averaging 0.683. The genotypes revealed by each marker were 15.78 in average. According to the analysis results, the similarity coefficient was at the level of 0.63. They were divided into 5 groups.【Conclusion】There is an obvious genetic diversity in wild Auricularia auricula strains and cultivated ones, this technique can be used to analyze the genetic diversity in the future study. |
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| Keywords: | Auricularia auricula SRAP genetic diversity |
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