首页 | 官方网站   微博 | 高级检索  
     

24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响
引用本文:康云艳,杨暹,郭世荣,张营营.24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响[J].中国农业科学,2011,44(12):2495-2503.
作者姓名:康云艳  杨暹  郭世荣  张营营
作者单位:南京农业大学园艺学院/农业部南方蔬菜遗传改良重点开放实验室;
基金项目:国家“973”计划项目(2009CB119000); 国家自然科学基金项目(30871763); 农业部现代农业产业技术体系建设专项资金资助项目(CARS25-C-03); 2010年广东高校优秀青年创新人才培养计划(育苗工程)
摘    要: 【目的】探讨外源EBR(24-表油菜素内酯)对低氧胁迫下黄瓜(Cucumis sativus L.)幼苗碳水化合物代谢的影响。【方法】采用营养液水培法,以耐低氧能力较强的黄瓜品种‘绿霸春4号’和耐低氧能力较弱的品种‘中农8号’为材料,研究根际低氧胁迫下外源施用EBR对黄瓜幼苗碳水化合物含量及根系糖酵解代谢酶活性的影响。【结果】通气条件下正常栽培,EBR处理显著促进两黄瓜品种叶片和根系中可溶性总糖的积累,但对根系糖酵解代谢酶活性的影响较小;低氧胁迫下,耐低氧能力不同的两品种幼苗根系淀粉、葡萄糖含量及酸性转化酶、ATP依赖的磷酸果糖激酶活性的变化表现出明显的品种差异;低氧条件下,EBR处理进一步提高了两品种根系中蔗糖和果糖含量及主要的糖酵解代谢酶活性,对叶片中大部分碳水化合物含量均无显著影响。【结论】低氧胁迫下,外源EBR通过促进碳水化合物从叶片向根系的分配及根系糖酵解代谢酶活性的提高,增强黄瓜植株耐低氧胁迫的能力。

关 键 词:黄瓜  24-表油菜素内酯  低氧胁迫  碳水化合物  糖酵解  
收稿时间:2010-10-13;

Effects of 24-Epibrassinolide on Carbohydrate Metabolism and Enhancement of Tolerance to Root-Zone Hypoxia in Cucumber(Cucumis sativus L.)
KANG Yun-yan,YANG Xian,GUO Shi-rong,ZHANG Ying-ying.Effects of 24-Epibrassinolide on Carbohydrate Metabolism and Enhancement of Tolerance to Root-Zone Hypoxia in Cucumber(Cucumis sativus L.)[J].Scientia Agricultura Sinica,2011,44(12):2495-2503.
Authors:KANG Yun-yan  YANG Xian  GUO Shi-rong  ZHANG Ying-ying
Affiliation:KANG Yun-yan1,2,YANG Xian2,GUO Shi-rong 1,ZHANG Ying-ying2 (1College of Horticulture,Nanjing Agricultural University/ Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,2College of Horticulture,South China Agricultural University,Guangzhou 510642)
Abstract:【Objective】The aim of the work was to study the effects of 24-epibrassinolide (EBR) added to nutrient solution on carbohydrate metabolism of cucumber (Cucumis sativus L.) under root-zone hypoxia. 【Method】Seedlings of a relatively hypoxia-resistant cultivar, ‘Lübachun No.4’, and a relatively hypoxia-sensitive cultivar, ‘Zhongnong No.8’, were hydroponically grown for 8 days in normoxic and hypoxic nutrient solutions with and without addition of EBR at 1 μg L-1. And the effects of EBR on contents of carbohydrates in leaves and roots and activities of glycolytic enzymes in the roots were investigated. 【Result】Application of EBR to normoxic nutrient solution promoted the accumulation of total soluble sugars in leaves and roots, but had no effects on activities of glycolytic enzymes in the roots. Changes in contents of starch and glucose, and activities of acid invertase and ATP-dependent phosphofructokinase enzymes in the roots showed obviously different between two genotypes cucumber in response to root-zone hypoxia. EBR added to hypoxic nutrient solution caused an increase in the concentrations of fructose and sucrose and activities of major glycolytic enzymes in the roots, but exerted little influence on carbohydrate concentrations in the leaves. 【Conclusion】These results suggest that EBR added to hypoxic nutrient solution may stimulate the photosynthate allocation down to roots and activities of major glycolytic enzymes, and eventually enhanced tolerance of cucumber plants to root-zone hypoxia.
Keywords:cucumber(Cucumis sativus L  )  24-epibrassinolide  hypoxia stress  carbohydrates  glycolysis  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国农业科学》浏览原始摘要信息
点击此处可从《中国农业科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号