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小麦果聚糖合成酶基因6-SFT-A单核苷酸多态性分析及其定位
引用本文:岳爱琴,李昂,毛新国,昌小平,李润植,贾继增,景蕊莲.小麦果聚糖合成酶基因6-SFT-A单核苷酸多态性分析及其定位[J].中国农业科学,2011,44(11):2216-2224.
作者姓名:岳爱琴  李昂  毛新国  昌小平  李润植  贾继增  景蕊莲
作者单位:山西农业大学农学院
基金项目:国家“973”计划项目(2010CB951501); CGIAR挑战计划项目(GCP,G7010.02.01)
摘    要: 【目的】小麦果聚糖合成酶基因6-SFT是果聚糖合成过程中的关键酶基因,研究6-SFT-A的多态性,分析其与小麦苗期抗旱性的关系,并进行遗传定位。【方法】以苗期抗旱性不同的30份六倍体小麦和4份小麦A基因组供体种乌拉尔图小麦为材料,通过直接测序分析6-SFT-A的单核苷酸多态性(SNP)及其与抗旱性的关系;开发基因分子标记,利用RIL群体(偃展1号×内乡188)对该基因进行遗传定位。【结果】在30份六倍体小麦材料中检测到14个核苷酸多态性位点,包括13个SNP和1个InDel,平均234 bp检测到一个多态性位点,仅在1 727和1 781 bp 2个位点检测到非同义突变;在4份乌拉尔图小麦中检测到28个SNP和4个InDel,其频率明显高于普通小麦。该基因的内含子1、2、3和外显子3为变异富集区,其它区域变异较小,外显子2变异最小,π值为0。34份材料分为3种单倍型,HaplⅠ主要包括中等抗旱材料和水敏感材料,Hapl Ⅲ中主要包括强抗旱材料和中等抗旱材料。利用RIL群体将该基因定位于染色体4A的标记Xcwm-27与Xwpt688之间,遗传距离分别为5.3和7.9 cM。【结论】单倍型分析表明,小麦果聚糖合成酶基因6-SFT-A单倍型与小麦苗期抗旱性有一定的相关性。

关 键 词:小麦  6-SFT-A  单核苷酸多态性  抗旱性  单倍型  遗传作图
收稿时间:2011-01-07;

Single Nucleotide Polymorphism and Mapping of 6-SFT-A Gene Responsible for Fructan Biosynthesis in Common Wheat
YUE Ai-qin,LI Ang,MAO Xin-guo,CHANG Xiao-ping,LI Run-zhi,JIA Ji-zeng,JING Rui-lian.Single Nucleotide Polymorphism and Mapping of 6-SFT-A Gene Responsible for Fructan Biosynthesis in Common Wheat[J].Scientia Agricultura Sinica,2011,44(11):2216-2224.
Authors:YUE Ai-qin  LI Ang  MAO Xin-guo  CHANG Xiao-ping  LI Run-zhi  JIA Ji-zeng  JING Rui-lian
Affiliation:YUE Ai-qin1,2,LI Ang2,MAO Xin-guo2,CHANG Xiao-ping2,LI Run-zhi1,JIA Ji-zeng2,JING Rui-lian2(1Agronomy College,Shanxi Agricultural University,Taigu 030801,Shanxi,2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm and Utilization,Ministry of Agriculture,Beijing 100081)
Abstract:Abstract: 【Objective】6-SFT is a key gene responsible for the fructan biosynthesis in plants. The objective of the present study was to detect single nucleotide polymorphism (SNP) of 6-SFT-A, determine the relationship between SNP and drought resistance, and mapping it on chromosome of wheat (Triticum aestivum L.). 【Method】Thirty hexaploid wheat accessions with different drought resistance at seedling stage and four T. urartu, the A genome donor species of hexaploid wheat, were selected to detect the nucleotide polymorphism in 6-SFT-A gene by sequencing, and determine the relationship between SNP and drought resistance. Using the recombinant inbred lines (RIL) derived from a cross Yanzhan 1 × Neixiang 188 mapped 6-SFT-A on chromosome. 【Result】Among the 30 hexaploid accessions, 14 polymorphism sites in 6-SFT-A nucleotide sequences were identified, including 13 SNP and one InDel (insertion and deletion) with the polymorphism frequency of 1/234 bp, only two nonsynonymous mutation identified in 1 727 bp and 1 781 bp sites. The introns 1, 2, 3 and exon 3 were detected as the rich nucleotide variation regions. Exon 2 showed the least variation. Compared to the common wheat, T. urartu species exhibited higher sequence variation. Totally, three haplotypes were identified for the plant materials. HaplotypeⅠconsists of wheat accessions with medium-drought resistance and drought sensitivity. Haplotype Ⅲ includes the wheat accessions with high- and medium-drought resistance. Using a population of 190 recombinant inbred lines derived from a cross of Yanzhan 1 × Neixiang 188, 6-SFT-A was mapped on chromosome 4A between SSR markers Xcwm-27 and Xwpt 688, and the genetic distances were 5.3 cM and 7.9 cM from the flanking markers, respectively. 【Conclusion】The present data indicated that SNP in 6-SFT-A gene is associated with wheat seedling drought resistance to some extent.
Keywords:wheat(Triticum aestivum L  )  6-SFT-A  single nucleotide polymorphism(SNP)  drought resistance  haplotype  genetic mapping  
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