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中国小麦地方品种春化基因的分布及其与冬春性的关系
引用本文:姜莹,黄林周,胡银岗.中国小麦地方品种春化基因的分布及其与冬春性的关系[J].中国农业科学,2010,43(13):2619-2632.
作者姓名:姜莹  黄林周  胡银岗
作者单位:(西北农林科技大学农学院)
基金项目:国家"863"重点项目子课题,科技部"973"计划前期项目,高等学校学科创新引智计划项目,澳大利亚ACIAR项目
摘    要:【目的】了解春化作用相关基因在中国小麦地方品种中的分布特点及其与小麦冬春性的关系,促进小麦地方品种的合理利用。【方法】采用小麦春化作用相关基因Vrn-A1、Vrn-B1、Vrn-D1和Vrn-B3的STS分子标记,对其在中国小麦十大生态栽培区的153份地方品种中的分布进行检测,并分析其与冬春性的关系。【结果】(1)中国小麦地方品种中4个显性春化基因的分布频率依次为60.78%(Vrn-D1)、5.88%(Vrn-A1a)、5.23%(Vrn-B1)和0(Vrn-B3)。(2)Vrn-A1a和Vrn-B1在东北春麦区等春麦区地方品种中的分布频率较高,以东北春麦区最高,达50%和33.33%,在大部分冬麦区未检测到这2个显性突变。10个麦区地方品种均检测到Vrn-D1,在青藏春冬麦区地方品种分布频率最高(83.33%),也是在中国冬麦区小麦地方品种中检测到的主要春化基因类型。(3)中国十大麦区地方品种的春化基因型与其对春化作用的要求基本吻合,除中部和南方冬麦区地方品种基因型与文献记载的冬春性的一致性指数较低外,其它麦区的冬春性一致性指数较高。【结论】通过分子标记检测,明确了中国小麦地方品种的春化基因类型及其分布特征,分子检测与田间观察相结合能够更准确地反映品种的冬春性。

关 键 词:  小麦" target="_blank">face="Verdana">小麦  地方品种  春化基因  分布频率  冬春性
收稿时间:2010-01-08;

Distribution of Vernalization Genes in Chinese Wheat Landraces and Their Relationship with Winter Hardness
JIANG Ying,HUANG Lin-zhou,HU Yin-gang.Distribution of Vernalization Genes in Chinese Wheat Landraces and Their Relationship with Winter Hardness[J].Scientia Agricultura Sinica,2010,43(13):2619-2632.
Authors:JIANG Ying  HUANG Lin-zhou  HU Yin-gang
Affiliation:(College of Agronomy, Northwest Agriculture and Forest University)
Abstract:【Objective】 The objective of this experiment is to understand the distribution of vernalization genes in Chinese wheat landraces and their relations with winter hardness so as to promote the reasonable utilization of those landraces in wheat improvement. 【Method】 Using STS markers of wheat vernalization genes Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3, the loci distribution of those genes among 153 Chinese wheat landraces from 10 major wheat ecological regions of China were detected, and their relationship with winter hardness was analyzed. 【Result】 The frequencies of the dominant loci of the four vernalization genes in Chinese wheat landraces were 60.78% (Vrn-D1), 5.88% (Vrn-A1a), 5.23% (Vrn-B1), and 0 (Vrn-B3), respectively. The higher frequencies of Vrn-A1a and Vrn-B1 were detected in spring wheat regions, with the highest in Northeastern China Spring Wheat Region with 50% and 33.33%, respectively, while in most winter wheat regions, those loci weren’t detected. Vrn-D1 was detected in all the 10 wheat ecological regions, which also was the main locus of vernalization genes in the landraces of winter wheat regions in China and the highest frequency was detected in Qinghai-Tibet Spring-Winter Wheat Region (83.33%). The genotypes of vernalization genes in wheat landraces from 10 major wheat ecological regions of China detected by molecular marker analysis were largely consistent with their vernalization requirement, and the consistency index of genotypes detected with the winter hardness recorded from the field was higher in general, while the consistency index for the landraces from the winter wheat regions of central and southern China were lower. 【Conclusion】The types and distribution characters of vernalization genes in Chinese wheat landraces were clarified, combining molecular detection of genotype and field investigation of phenotype could reflect the winter hardness of wheat better.
Keywords:wheat  landraces  vernalization genes  distribution frequency  winter hardness
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