| 一个谷子新抗锈基因的AFLP标记 |
| |
| 引用本文： | 赵立强,潘文嘉,马继芳,瓮巧云,董立,全建章,邢继红,董志平,董金皋.一个谷子新抗锈基因的AFLP标记[J].中国农业科学,2010,43(21):4349-4355. |
| |
| 作者姓名： | 赵立强 潘文嘉 马继芳 瓮巧云 董立 全建章 邢继红 董志平 董金皋 |
| |
| 作者单位： | (河北省农林科学院谷子研究所) |
| |
| 基金项目： | 国家自然科学基金(30771354); 河北省自然科学基金重点项目(C2006000723); 河北省杰出青年基金(C2009001540); 国家“十一五”科技支撑计划项目(2006BAD02B02-02) |
| |
| 摘 要： | 【目的】研究谷子抗源的抗锈遗传规律,寻找和定位与谷子抗锈基因连锁的分子标记,为谷子抗锈病基因的定位、克隆和抗病育种等研究奠定基础。【方法】用谷子锈菌单胞菌系93-5接种十里香和豫谷1号及杂交后代F1、F2进行抗锈鉴定,并根据鉴定结果构建抗、感基因池;利用AFLP技术筛选128对EcoRⅠ/MseⅠ引物组合,从中寻找和定位与谷子抗锈基因连锁的分子标记;根据AFLP分析结果进行抗锈基因连锁分析并进行SCAR标记转化。【结果】根据十里香×豫谷1号杂交后代F2群体(131株)抗感谷锈病分离比例,确定十里香抗锈性由显性单基因控制。筛选获得3个与谷子抗锈基因Rusi1(暂命名)连锁的AFLP分子标记,经计算标记与该抗锈基因的遗传距离分别为7.4、9.2和27.4cM。将3个标记片段回收、克隆和测序,成功地将AFLP标记E+CTT/M+TAC-256转化为SCAR标记。初步构建了谷子抗锈基因Rusi1的遗传连锁图谱。【结论】谷子十里香抗锈性由显性单基因控制,Rusi1是一个新发现的谷子抗锈基因。
|
| 关 键 词： | 谷子" target="_blank">face="Verdana">谷子 谷子锈病 抗锈基因 分子标记 遗传连锁图 |
| 收稿时间： | 2010-04-26; |
Identification of AFLP Markers Linked to a Novel Rust Resistance Gene in Foxtail Millet |
| |
| ZHAO Li-qiang,PAN Wen-jia,MA Ji-fang,WENG Qiao-yun,DONG Li,QUAN Jian-zhang,XING Ji-hong,DONG Zhi-ping,DONG Jin-gao.Identification of AFLP Markers Linked to a Novel Rust Resistance Gene in Foxtail Millet[J].Scientia Agricultura Sinica,2010,43(21):4349-4355. |
| |
| Authors: | ZHAO Li-qiang PAN Wen-jia MA Ji-fang WENG Qiao-yun DONG Li QUAN Jian-zhang XING Ji-hong DONG Zhi-ping DONG Jin-gao |
| |
| Affiliation: | ZHAO Li-qiang1,2,PAN Wen-jia2,MA Ji-fang1,WENG Qiao-yun3,DONG Li1,QUAN Jian-zhang1,XING Ji-hong2,DONG Zhi-ping1,DONG Jin-gao2 (1Institute of Millet Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,2College of Life Science,Agricultural University of Hebei,Baoding 071001,Hebei,3Hebei North University,Zhangjiakou 075131,Hebei) |
| |
| Abstract: | 【Objective】 The AFLP makers linked to rust resistance gene in foxtail millet were studied as they are useful for new resistance genes and the rational usage of marker-assisted selection in millet resistance breeding. 【Method】 A cross between resistant cultivar ‘Shilixiang’ and susceptible cultivar ‘Yugu 1’ was used to map foxtail millet rust resistance gene(s). Based on the identification result of F1 and F2 progenies inoculated with the Uromyces setariae-italicae Yoshino strains 93-5, the resistant and susceptible pools were established. With 128 EcoRⅠ/MseⅠ primer combinations, AFLP analysis was performed on parents and two bulks. AFLP makers were applied to construct a genetic linkage map of resistance gene in an F2 population of 131 individuals, which were sequenced and then converted to stable SCAR markers. 【Result】 The inoculation test showed that the resistance of ‘Shilixiang’ to millet rust was controlled by one dominant gene. In 128 AFLP primer combinations, three markers were found to be linked to the rust resistance gene, with the genetic distance of 7.4, 9., and 27.4 cM, respectively, which was tentatively named Rusi1. A preliminary genetic linkage map was constructed using 3 AFLP markers. Furthermore, the three cloned fragments were sequenced and then E+CTT/M+TAC-256 was successfully converted to a stable SCAR marker. Genetic linkage map of foxtail millet rust resistance gene Rusi1 was constructed. 【Conclusion】 The resistance to millet rust in ‘Shilixiang’ is controlled by a single dominant gene. The gene Rusi, derived from ‘Shilixiang’, is a rust resistance gene found in foxtail millet. |
| |
| Keywords: | Setaria italica (L ) Beauv millet rust rust resistance gene molecular marker genetic linkage map |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
|
