| 水稻品种与稻瘟病菌分子指纹聚类互作分析初探 |
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| 引用本文： | 范静华,汪安云,陈建斌,王云月,孙鹰,王宗华,朱有勇.水稻品种与稻瘟病菌分子指纹聚类互作分析初探[J].中国农业大学学报,1998(Z1). |
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| 作者姓名： | 范静华 汪安云 陈建斌 王云月 孙鹰 王宗华 朱有勇 |
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| 作者单位： | 云南农业大学云南省植物病理重点实验室;菲律宾国际水稻所;云南农业大学云南省植物病理重点实验室 昆明; |
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| 基金项目： | 云南省攻关项目; 国际水稻所合作项目资助 |
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| 摘 要： | 本试验进行了41个水稻品种的 GRA-PCR 和15个稻瘟病菌的 REP-PCR 分子指纹扩增和聚类分析;完成了品种和病菌互作人工接种试验。结果表明,供试品种的 GRA-PCR 指纹聚类后,以0.56遗传相似距离点划分时,供试的41个品种可分为两个遗传相似组,其中一组的19个品种大多数为云南省地方老品种,另一组的23个品种多数为近年来生产上的栽培品种。病菌 REP-PCR 指纹聚类后,在不同的相似距离划分时,获得不同的遗传相似组,当以0.08相似距离划分时,各遗传相似组的致病性与供试品种之间有一定的互作关系。
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| 关 键 词： | 稻瘟病 互作 GRA-PCR REP-PCR |
Cluster Analysis on DNA Fingerprints of Rice Cultivars and Blast Isolates and Their Relationship |
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| Fan Jinghua Wan Anyun Chen Jianbin Wang Yunyue Sun Ying Zhu Youyong The Phytopathology Lab.of Yunnan Province,Kunming ,P.R.China Wang Zonghua Internation Rice Research Institute,P.O.Box.,Manila ,Philippines.Cluster Analysis on DNA Fingerprints of Rice Cultivars and Blast Isolates and Their Relationship[J].Journal of China Agricultural University,1998(Z1). |
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| Authors: | Fan Jinghua Wan Anyun Chen Jianbin Wang Yunyue Sun Ying Zhu Youyong The Phytopathology Labof Yunnan Province Kunming PRChina Wang Zonghua Internation Rice Research Institute POBox Manila Philippines |
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| Affiliation: | Fan Jinghua Wan Anyun Chen Jianbin Wang Yunyue Sun Ying Zhu Youyong The Phytopathology Lab.of Yunnan Province,Kunming 650201,P.R.China Wang Zonghua Internation Rice Research Institute,P.O.Box.933,Manila 1099,Philippines |
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| Abstract: | The genomic DNA of 41 varieties and 15 blast isolates were amplified using GRA-PCR and REP-PCR respectively,and clustered with NTSY-UPGRAM program. Then these varieties were tested by inoculation with isolates in this study.The results showed that 41 varieties were clustered into 2 genetic similar groups cutting off at 0.56.19 old varieties of Yunnan fell in group A and the other 23 varieties in group B.15 isolates in this study were clustered into different groups when cut off at different distance.There w... |
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| Keywords: | rice blast relationship GRA-PCR REP-PCR |
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