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感染鳗弧菌对花鲈非特异性免疫功能的影响
引用本文:郭伟荣,刘利平,张宗锋,林升.感染鳗弧菌对花鲈非特异性免疫功能的影响[J].上海海洋大学学报,2011,20(1):89-95.
作者姓名:郭伟荣  刘利平  张宗锋  林升
作者单位:上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室;上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室;上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室;上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室
基金项目:上海市教育委员会科研(创新)项目(09YZ277);上海市青年科技启明星项目(08QA1405900);上海市重点学科建设项目(Y1101);美国国际发展署(USAID);AquaFish CRSP项目(3001325371)
摘    要:鳗弧菌(Vibrio anguillarum)是海水鱼常见的一种细菌性病。以A 组:1*106 cfu/mL、B组:1*107 cfu/mL、C组:1*108 cfu/mL 3组不同浓度的鳗弧菌菌液通过腹腔注射感染花鲈(Lateolabrax japonicus),在其感染后不同时间取血,通过测定呼吸爆发、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、酚氧化酶(PO)以及超氧化物歧化酶(SOD)活力的变化趋势来研究感染鳗弧菌对花鲈的非特异性免疫功能的影响。结果表明:呼吸爆发在鳗弧菌感染12 h、36 h时,B组和C组与对照组比较极显著下降(P<0.01);PO活性,在鳗弧菌感染12 h、36 h、60 h、84 h、108 h时,A组、B组、C组与对照组比较差异极显著下降(P<0.01);ALP活性,在鳗弧菌感染60 h时,A组、B组、C组与对照组比较差异极显著下降(P<0.01);ACP活性,在60 h、84 h、108 h时,A组、B组、C组与对照组比较差异显著升高(P<0.05);SOD活性,在鳗弧菌感染12 h、36 h、60 h、84 h、108 h时,无显著性差异(P>0.05)。此外,在半致死的实验中,鳗弧菌浓度为1*106 cfu/mL、1*107 cfu/mL 、1*108 cfu/mL的实验组的50%的致死时间分别为12 d、11 d、8 d,对照组则无死亡。结果表明:在感染期间,实验组鱼表现出特定的症状,如尾鳍溃烂、鳃丝发白、内部有黄色粘稠腹水等;鳗弧菌浓度越高,达到50%的致死时间越早,花鲈的死亡率和鳗弧菌的浓度成正相关。

关 键 词:花鲈  鳗弧菌  非特异性免疫  半致死时间

The impact of Vibrio anguillarum challenging on non specific immune responses of sea perch Lateolabrax japonicus
GUO Wei-rong,LIU Li-ping,ZHANG Zong-feng and LIN Sheng.The impact of Vibrio anguillarum challenging on non specific immune responses of sea perch Lateolabrax japonicus[J].Journal of Shanghai Ocean University,2011,20(1):89-95.
Authors:GUO Wei-rong  LIU Li-ping  ZHANG Zong-feng and LIN Sheng
Abstract:Vibrio anguillarum is a common form of bacterial pathogens for marine fish. In this paper, sea perch (Lateolabrax japonicus) was challenged with an intraperitoneal injection at different concentrations of V. anguillarum: namely treatments A: 1*106 pfu/mL, B:1*107 pfu/mL, C:1*108 pfu/mL and the innate immunity indicators were investigated. Blood samples were taken at different time, and the effects of challenging of V. anguillarum on the immunity responses were studied by measuring the respiratory burst, alkaline phosphatase (ALP), acid phosphatase (ACP), phenoloxidase (PO) and superoxide dismutase (SOD) activities. The results showed that: 1) Compared with the control group, the respiratory burst activity of treatment B and C decreased significantly (P<0.01) at 12h and 36h of injection. 2) Compared with the control group, the PO activity of treatment A, B and C at 12 h, 36 h ,60 h, 84 h and 108 h after injection decreased significantly (P<0.01). 3) There was no significant difference between treatments and control groups for ALP activity except for at 60 h. 4) Compared with the control group, the ACP activity of treatment A, B and C at 60 h, 84 h and 108 h after injection increased significantly (P<0.05). 5) There was no significant difference between treatments and control groups for SOD activity at 12 h, 36 h, 60 h, 84 h and 108 h(P>0.05). Moreover, in the semi lethal experiment, V. anguillarum at the concentration of 1*106 pfu/mL,1*107 pfu/mL and 1*108 pfu/mL 50%, of death in experimental group occurred on 12 d,11 d and 8d respectively, while no death for control group. During infection, experimental group has different symptoms: caudal fin ulceration, gill pale, whitish cornea, the mouth's ulceration, the inside has yellow viscous ascites. The higher the concentration infection with V. anguillarum, the earlier was for 50% of the death. These results indicated that V. anguillarum concentration and the mortality rate are positively correlated.
Keywords:Lateolabrax japonicus  Vibrio anguillarum    innate immunity  semilethal time
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