| 高效液相色谱法测定鳗鱼中氟甲喹和喹酸的残留量 |
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| 引用本文： | 刘艳萍,冷凯良,王清印,王志杰,孙伟红,谭志军,郭萌萌.高效液相色谱法测定鳗鱼中氟甲喹和喹酸的残留量[J].上海海洋大学学报,2009(3):332-337. |
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| 作者姓名： | 刘艳萍 冷凯良 王清印 王志杰 孙伟红 谭志军 郭萌萌 |
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| 作者单位： | [1]中国水产科学研究院黄海水产研究所,青岛266071; [2]上海海洋大学水产与生命学院,上海201306 |
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| 基金项目： | 基金项目：农业部行业专项（NYhyzx07-046）;国家“八六三”高技术研究发展计划（2008AA100805） |
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| 摘 要： | 建立了高效液相色谱-荧光检测器法测定鳗鱼中氟甲喹和噁喹酸残留量的方法。用乙腈萃取鳗鱼样品中氟甲喹和噁喹酸残留物。色谱柱采用C8Luna，柱温为35℃，激发波长和发射波长分别为312nm和369nm，流动相为0．01mol/L草酸：乙腈=65：35（V/V）。氟甲喹和噁喹酸标准曲线在线性范围1～100ng/mL时呈良好线性关系。对带皮的鳗鱼肉进行3种浓度的回收率测定。实验结果表明，回收率80％～110％，变异系数≤10％。氟甲喹的检测限为2．5μg／kg，定量下限为5μg／kg；噁喹酸的检测限为1μg／kg，定量下限为2μg／kg。整个提取过程步骤简单，易于操作，可以满足世界各国目前对出口水产品检测的限量要求。
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| 关 键 词： | 氟甲喹 噁喹酸 鳗鱼 高效液相色谱 |
Determination of flumequine and oxolinic acid in eel with HPLC method |
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| LIU Yan-ping,LENG Kai-liang,WANG Qing-yin,WANG Zhi-jie,SUN Wei-hong,TAN Zhi-jun,GUO Meng-meng.Determination of flumequine and oxolinic acid in eel with HPLC method[J].Journal of Shanghai Ocean University,2009(3):332-337. |
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| Authors: | LIU Yan-ping LENG Kai-liang WANG Qing-yin WANG Zhi-jie SUN Wei-hong TAN Zhi-jun GUO Meng-meng |
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| Affiliation: | LIU Yan-ping, LENG Kai-liang, WANG Qing-yin , WANG Zhi-jie , SUN Wei-hong, TAN Zhi-jun , GUO Meng-meng ( 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China) |
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| Abstract: | A high-performance liquid chromatography（ HPLC ）- fluorescence detection method is presented for the determination of flumequine and oxolinic acid residues in eel tissues at excitation wavelength of 312 nm and emission wavelength of 368 nm. The HPLC separation was carried out on a C8Luna column with 0.01 mol/L oxalic acid and acetonitrile（65： 35） as the mobile phase. The pharmaceuticals were extracted from the matrix with acetonitrile solvent and analysed by HPLC-fluorescenee detector. Under the optimum conditions excellent linearity was obtained in the range 1 - 100 ng/g concentrations with satisfactory correlation coefficients. In fortification studies, the recoveries of the analytes were in the range of 80% - 110%. the coefficients of variation within batches were ≤10%. Limits of detection （LODs） were 2. 5 μg/kg for flumequine and 1 μg/kg for oxolinie acid, while limits of quantitation（LOQs） were 5.0 μg/kg for flumequine and 2.0 μg/kg for oxolinic acid. This method is suitable to verify surely flumequine and oxolinic acid residues testing in eel tissues. The method is suitable to verify surely flumequine and oxolinic acid residues testing in eel tissues. |
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| Keywords: | flumequine oxolinic acid eel HPLC |
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