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三个牛品种MBL1基因第一内含子与第二外显子遗传多态性研究
引用本文:刘梅,鞠志花,李秋玲,王洪梅,黄金明,李建斌,仲跻峰,王长法.三个牛品种MBL1基因第一内含子与第二外显子遗传多态性研究[J].中国农业科技导报,2010,12(1):72-78.
作者姓名:刘梅  鞠志花  李秋玲  王洪梅  黄金明  李建斌  仲跻峰  王长法
作者单位:(1.山东省农业科学院奶牛研究中心, 济南 250100,2.山东师范大学生命科学学院, 济南 250014)
基金项目:国家863计划项目(2006AA10Z1D9,2007AA10Z169);;现代农业产业技术体系项目(nycytx-0107);;山东省良种工程项目(2007LZ015-06);;山东省农业科学院创新基金项目(2006YCX028)资助
摘    要:采用巢式PCR、DNA测序和CRS-PCR方法,研究中国荷斯坦牛、鲁西黄牛、渤海黑牛的MBL1基因内含子1和外显子2的单核苷酸多态性(SNPs),发现了855(G/A)、2651(G/A)和2686(T/C)3个新SNP位点。855(G/A)位于内含子1上,2651(G/A)导致Val24Ile氨基酸的改变,2686(T/C)为同义突变。3个SNP位点在3个牛品种群体中优势等位基因相同,分别为G、G、C,其等位基因频率分别为0.87/0.58/0.57、1/0.75/074、1/0.76/0.63。经χ2适合性检验,荷斯坦牛在855(G/A)位点、鲁西黄牛在855(G/A)、2651(G/A)位点、渤海黑牛的所有位点达到Hardy-Weinberg平衡状态(P>0.05)。3个牛品种在855(G/A)位点均表现为低度多态;在2651(G/A)和2686(T/C)位点均表现为中度多态(0.25
关 键 词:  MBL1基因  遗传多态性  优势等位基因  
收稿时间:2009-11-16
修稿时间:2009-12-22

Studies on Polymorphisms Within Intron 1 and Exon 2 of MBL1 Gene in Three Cattle Breeds
LIU Mei,JU Zhi-hua,LI Qiu-ling,WANG Hong-mei,HUANG Jin-ming,LI Jian-bin,ZHONG Ji-feng,WANG Chang-fa.Studies on Polymorphisms Within Intron 1 and Exon 2 of MBL1 Gene in Three Cattle Breeds[J].Journal of Agricultural Science and Technology,2010,12(1):72-78.
Authors:LIU Mei  JU Zhi-hua  LI Qiu-ling  WANG Hong-mei  HUANG Jin-ming  LI Jian-bin  ZHONG Ji-feng  WANG Chang-fa
Affiliation:(1.Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100|2.Life Science College, Shandong Normal University, Jinan 250014, China)
Abstract:The single nucleotide polymorphisms (SNPs) within intron 1 and exon 2 of MBL1 gene in Chinese Holstein, Luxi cattle and Bohai cattle were investigated by using nested-PCR, DNA sequencing technology and CRS-PCR method. Sequencing results showed that three new SNPs were identified. They were 855(G/A)、2651(G/A) and 2686(T/C). 855(G/A)SNP was located in intron 1; 2651(G/A)caused amino acids alteration in Val24Ile; 2686(T/C)was synonymous mutation. Predominant allele were identical at three SNP sites in three cattle populations, which were G, G, C and the allelic frequencies were 0.87/0.58/0.57, 1/0.75/0.74, 1/0.76/0.63, respectively. By χ2 tests, we found that 855(G/A) of Chinese Holstein, 855(G/A) and 2651(G/A) of Luxi cattle and all sites of Bohai cattle were in accordance with Hardy-Weinberg equilibrium (P>0.05). The value of polymorphism information indicated that 855(G/A) was slight polymorphism; 2651(G/A)and 2686(T/C)were moderate polymorphism in the three cattle breeds(0.25<PIC<0.5).
Keywords:cattle  MBL1 gene  polymorphisms  predominant allele  
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