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低毒高效的植物石蜡切片方法
引用本文:李凤英,马姜明,梁士楚,唐绍清,薛跃规.低毒高效的植物石蜡切片方法[J].农学学报,2014,4(4):83-85.
作者姓名:李凤英  马姜明  梁士楚  唐绍清  薛跃规
作者单位:广西师范大学生命科学学院/珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004
基金项目:广西师范大学实验室管理创新与技术革新项目“石蜡切片实验方法的改进及其在探究实验中的应用”(2012GJY22);广西高等教育教学改革工程项目“充分利用高校生物园构建生态学专业实践性教学新模式”(2013JGA115);广西师范大学教育教学改革立项项目“《生态工程学》课程改革研究与实践”.
摘    要:以乔木、灌木和藤本等不同类型植物叶及部分根茎为试验材料,对石蜡切片的主要技术环节进行了探究和改进.用天然有机溶剂松节油(oil of turpentine)代替二甲苯(xylene)为透明与脱蜡剂,以阿拉伯树胶(gum acacia powder)为贴片剂,采用番红整体染色与固绿分片染色相结合进行双重染色.试验结果表明,该方法可避免二甲苯的毒性危害,减少环境污染,缩短试验所需的时间,提高劳动效率和制片质量.

关 键 词:石蜡切片  植物  低毒高效  方法
收稿时间:2013/9/29 0:00:00
修稿时间:2013/11/18 0:00:00

A Low-Toxicity and High-Effectiveness Preparation Method for Plant Paraffin Section
Li Fengying,Ma Jiangming,Liang Shichu,Tang Shaoqing,Xue Yuegui.A Low-Toxicity and High-Effectiveness Preparation Method for Plant Paraffin Section[J].Journal of Agriculture,2014,4(4):83-85.
Authors:Li Fengying  Ma Jiangming  Liang Shichu  Tang Shaoqing  Xue Yuegui
Affiliation:(College of Life Science, Guangxi Normal University/Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541004, Guangxi, China)
Abstract:In this paper, exploration and improvement to the primary technical section was discussed base on the material of various leaves and roots from several plants including trees, bush and liane. During staining, Turpentine was used as transparent agent, and arabic gum was employed as adhesive. The safranin and fast green were binatively utilized in the staining process. A wealth of results showed that the contamination from xylene could be avoided via our staining method, and the marked decline of pollution to environment was significant. Furthermore, the effectiveness and quality of the section were significantly improved with a shorter duration.
Keywords:Paraffin Section  Plants  Low Toxicity and High Effect  Method
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