| 高粱幼嫩叶段愈伤诱导与植株再生 |
| |
| 引用本文： | 郝艳芳,王良群,刘 勇,张 微,杨 伟,白鸿雁,武 擘.高粱幼嫩叶段愈伤诱导与植株再生[J].农学学报,2018,8(7). |
| |
| 作者姓名： | 郝艳芳 王良群 刘 勇 张 微 杨 伟 白鸿雁 武 擘 |
| |
| 作者单位： | 山西省农业科学院高粱研究所,山西省农业科学院高粱研究所,山西省农业科学院高粱研究所,山西省农业科学院高粱研究所,山西省农业科学院高粱研究所,山西省农业科学院高粱研究所,山西省农业科学院高粱研究所 |
| |
| 基金项目： | 山西省科技厅攻关项目“高粱组培育种技术在矮杆高产品种选育中的应用”(20150311003-2)；山西省农业科学院农业科技创新研究课题 “高粱悬浮细胞原生质体培养体系的研究”(ZDSYS1506)；高粱遗传与种质创新山西省重点实验室青年基金课题“农杆菌介导高粱遗传转化体系的建 立”(2018Q-5)。 |
| |
| 摘 要： | 目的]为了建立以幼嫩叶段为外植体的高粱组培技术。方法]选用育种上常用的8个高粱恢复系材料,进行愈伤组织诱导与分化再生,研究2,4-D浓度对初级愈伤组织诱导的影响,分别对8个材料的诱导率和分化率进行统计,并观察愈伤组织形态。结果]结果表明,2,4-D是幼嫩叶段诱导愈伤的关键因素,未添加2,4-D的培养基中不能产生愈伤,适宜的2,4-D浓度为2 mg/L。基因型对愈伤组织的质量和形态有很大的影响,不同基因型材料的诱导率和分化率存在很大差异。结论]通过比较,筛选出了3个诱导率高、愈伤形态优良且分化率较高的基因型三尺三、R111、8643变。
|
| 关 键 词： | 草炭 草炭 pH 碳酸钾 氧化钙 有效养分 幼苗生长 |
| 收稿时间： | 2018/2/13 0:00:00 |
| 修稿时间： | 2018/3/19 0:00:00 |
Callus Induction and Plant Regeneration from Young Leaf Segments of Sorghum bicolor |
| |
| Abstract: | To establish a sorghum tissue culture system with young leaf segments as explants, eight sorghum restorer lines commonly used in breeding are selected, and young leaf segments are used as explants for callus induction and plant regeneration. The effect of 2,4-D concentration on primary callus induction is studied. The callus induction and differentiation frequency are recorded, and the callus morphology is also observed. The results showed that 2,4-D was the key factor inducing callus of young leaves. No callus could be produced in medium without 2,4-D. The suitable 2,4-D concentration was 2 mg/L. Genotype had a significant effect on callus quality and morphology, and the callus induction and differentiation rate of these eight genotypes were very different. Three genotypes,‘Sanchisan’,‘R111’and‘8643 bian’, were selected after screening for their performance of higher callus induction rate, excellent morphology and higher differentiation rate. |
| |
| Keywords: | Sorghum bicolor Young Leaf Segments Embryogenesis Callus Induction Differentiation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《农学学报》浏览原始摘要信息 |
|
点击此处可从《农学学报》下载全文 |
|
