| 小麦抗麦红吸浆虫品种的遗传多样性分析 |
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| 引用本文： | 史忠良,仇松英,张松令,谢福来,高炜,张婷,逯腊虎,武计平.小麦抗麦红吸浆虫品种的遗传多样性分析[J].农学学报,2011,1(1):10-16. |
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| 作者姓名： | 史忠良 仇松英 张松令 谢福来 高炜 张婷 逯腊虎 武计平 |
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| 作者单位： | 山西省农业科学院小麦研究所,山西临汾,041000 |
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| 基金项目： | 山西省科技攻关项目，％％ |
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| 摘 要： | 为了更深入地揭示小麦抗麦红吸浆虫品种（系）的遗传多样性，在田间虫圃对1562份小麦品种（系）损失率鉴定结果的基础上，取47份年度间鉴定抗性结果较为一致的材料，按损失率大小，由小到大排列起来，利用19对多态性SSR标记检测了参试材料的遗传多态性。结果表明，19对SSR标记在47份不同抗性品种中检测到104个等位基因，能够将所有品种区分开来，每对引物可以检测到3-8个等位基因，平均5．47个。47个小麦品种间的遗传距离从0．40～0．95不等，平均为0．71。高抗品种间遗传距离平均为0．66（0．40-0．95）；中抗品种间的遗传距离平均为0．69（0．46～0．90）；感虫品种间的遗传距离平均为0．69（0．53-0．95）；高感品种间的遗传距离平均为0．68（0．41～0．90）。SSR标记聚类分析在遗传距离为0．74处将供试材料分为6大类群。抗虫品种晋麦65号单独聚为一类，同其余品种具有较远的亲缘关系，可作为新的抗源用于抗虫育种，并在吸浆虫发生地块推广种植。
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| 关 键 词： | 小麦 麦红吸浆虫 SSR标记 遗传多样性 遗传距离 |
Analysis on Genetic Diversity of Wheat Varieties with Resistance to Wheat Midge ( Sitodiplosis mosellana) |
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| Shi Zhongliang,Qiu Songying,Zhang Songling,Xie Fulai,Gao Wei,Zhang Ting,Lu Lahu,Wu Jiping.Analysis on Genetic Diversity of Wheat Varieties with Resistance to Wheat Midge ( Sitodiplosis mosellana)[J].Journal of Agriculture,2011,1(1):10-16. |
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| Authors: | Shi Zhongliang Qiu Songying Zhang Songling Xie Fulai Gao Wei Zhang Ting Lu Lahu Wu Jiping |
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| Affiliation: | (Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen 041000, Shanxi, China) |
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| Abstract: | In order to gain the knowledge of genetic diversity of wheat varieties with resistance to wheat midge （Sitodiplosis mosellana）, on the basis of field insect plot appraisal, 47 wheat varieties/lines were involved by 19 SSR markers to explore their genetic diversity. The 19 SSR markers were screened and a total of 104 allelic variants were detected in 47 wheat varieties. Three to eight alleles per marker were detected, with an average of 5.47. The means of alleles of high resistant, moderate resistant, sensitive and high sensitive varieties were 4.11, 4.68, 3.95 and 3.84, respectively. The PIC （polymorphism information content） for each polymorphic primer was varied from 0.35 to 0.86 with an average of 0.74. The PICs of high resistant, moderate, sensitive and high sensitive varieties had an average of 0.64 （0.31-0.81）, 0.69 （0.38-0.88）, 0.67 （0.40-0.80） and 0.67 （0.20-0.89）, respectively. The genetic distance （GD） of 47 varieties was ranged from 0.40 to 0.95, with an average of 0.71. The GDs of high resistant, moderate resistant and high sensitive varieties were varied from 0.40 to 0.95, from 0.46 to 0.90 and from 0.41 to 0.90, with an average of 0.66, 0.69 and 0.68. Based on cluster analysis results, the 47 varieties were clustered into six groups. Resistant variety ＇Jinmai 65＇ was a group by oneself, indicating that there was a farther genetic relationship between ＇Jinmai 65＇ and other varieties, and so the ＇Jinmai 65＇ might be used as resistant parent and might be popularized in production. |
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| Keywords: | Wheat Varieties： Wheat Midge Genetic Diversity SSR marker Genetic Distance |
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