| 东方百合明星无性快繁体系的建立 |
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| 引用本文： | 牛俊乐,杨美纯,王燕,农艳丰,岑湘涛.东方百合明星无性快繁体系的建立[J].南方农业学报,2016(9):1528-1532. |
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| 作者姓名： | 牛俊乐 杨美纯 王燕 农艳丰 岑湘涛 |
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| 作者单位： | 1. 广西大学 农学院，南宁 530005; 百色学院，广西 百色 533000;2. 广西大学 农学院,南宁,530005;3. 百色学院,广西 百色,533000 |
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| 摘 要： | 【目的】以东方百合品种明星无菌叶为材料建立快繁体系，为其种球产业化生产提供技术支撑。【方法】以花器官培养获得的无菌小鳞片及叶片为材料，以添加不同浓度6-BA、2,4-D的MS培养基诱导不定芽及小鳞茎产生；以不同浓度的蔗糖诱导小鳞茎的膨大及根的产生；以珍珠岩、草炭、蛭石的混合基质作为小鳞茎移栽基质。【结果】用6-BA诱导无菌小鳞片产生不定芽时以1.5 mg/L为宜，诱导率为75.0%；用2,4-D诱导无菌叶片产生小鳞茎时以2.0 mg/L为宜，诱导率为91.7%；添加70.0 g/L蔗糖的MS培养基适宜小鳞茎膨大及根的产生；带小鳞茎及根的组培苗在珍珠岩∶草炭∶蛭石=1∶1∶1的基质中移栽成活率最高，达100.0%。【结论】东方百合品种明星可利用无菌叶片建立无性快繁体系。
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| 关 键 词： | 东方百合明星 无菌叶 小鳞茎 无性快繁 |
Asexually rapid propagation system established of Oriental lily Love Story |
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| Abstract: | Objective]Propagation system established with sterile leaves of Oriental lily Love Story as material to provide technical reference for industrialized production of its seed-bulbs. Method]Sterile scales and leaves from flower organs culture were used as material and MS was used as basic medium, adding different concentrations of 6-BA and 2,4-D to induce adventitious buds and bulb-lets; using different concentrations of sugar to induce bulb-lets expansion and rooting; perlite, peat and vermiculite were mixed as the transplanted matrix of bulb-lets. Result]MS+1.5 mg/L 6-BA was appropriate for inducing adventitious buds with sterile scales and induction rate was 75.0% ; the suitable sterile leaves differentiation bulb -lets medium was MS +2.0 mg/L 2,4-D and induction rate was 91.7% ; the MS medium added 70.0 g/L sugar was suitable to induce bulb-lets expansion and rooting; perlite ∶peat∶vermiculite=1∶1∶1 was the best transplanted matrix of plant-lets with bulb-lets and roots, transplanting survival rate got 100.0% in this matrix.Conclusion]Oriental lily Love Story could be established asexually rapid propagation system by sterile leaf. |
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| Keywords: | Oriental lily Love Story tissue culture bulblets rapid propagation |
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