| MyoG基因多态性与瑶山鸡体尺、屠宰性能及肉质性状的关联分析 |
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| 引用本文： | 杨德凤,李维,刘洋,张芸,龚俞,张蓝艺,陈颖,林家栋,张福平,唐继高.MyoG基因多态性与瑶山鸡体尺、屠宰性能及肉质性状的关联分析[J].南方农业学报,2021,52(10):2887-2895. |
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| 作者姓名： | 杨德凤 李维 刘洋 张芸 龚俞 张蓝艺 陈颖 林家栋 张福平 唐继高 |
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| 作者单位： | 1 贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025;2 贵州大学科研鸡场, 贵阳 550025;3 贵州大学家禽研究所, 贵阳 550025;4 贵州省畜禽遗传 资源管理站, 贵阳 550001;5 贵州农业职业学院, 贵阳 551400;6 贵州省畜牧兽医研究所, 贵阳, 550005 |
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| 基金项目： | 贵州省科技计划项目（黔科合支撑〔2017〕2533-1） |
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| 摘 要： | 【目的】探究肌细胞生成素基因（MyoG）多态性与瑶山鸡体尺、屠宰性能及肉质性状的关联性，从分子标记角度对瑶山鸡进行选育，更好地保护和开发利用瑶山鸡，同时为家禽MyoG基因研究提供参考依据。【方法】以150日龄出栏的健康瑶山鸡为研究对象，通过PCR扩增产物直接测序对瑶山鸡MyoG基因多态性进行检测，利用DNAStar对测序结果进行比对分析以确定MyoG基因SNP位点，通过RNAfold对瑶山鸡MyoG基因突变前后的mRNA二级结构进行预测，并以SPSS 18.0分析SNP位点与瑶山鸡体尺、屠宰性能及肉质性状的相关性。【结果】在瑶山鸡MyoG基因第3外显子上发现1个SNP位点（T17C位点），属于同义突变，存在3种基因型（CC型、CT型和TT型）；T为优势等位基因，CT为优势基因型；有效等位基因数（Ne）接近2.00；多态信息含量（PIC）为0.35，呈中度多态（0.25P>0.05）。MyoG基因T17C位点突变致使其mRNA二级结构发生改变，进而导致其最小自由能由-452.60 kcal/mol降至-465.24 kcal/mol。不同基因型瑶山鸡生长、屠宰性能及肉质性状间的相关分析结果显示，3种基因型瑶山鸡在体尺、屠宰性能及肉质性状间的相关性大部分呈现相同规律，其中胸宽与胸深在不同基因型瑶山鸡中均呈极显著正相关（P<0.01）。【结论】瑶山鸡MyoG基因T17C位点存在3种基因型，属于同义突变，并未引起氨基酸及其蛋白结构和功能的明显变化，但其多态性对瑶山鸡体尺性状、屠宰性能和肉质性状有一定影响，因此可作为瑶山鸡分子选育的候选遗传标记。
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| 关 键 词： | 瑶山鸡 肌细胞生成素基因(MyoG) 多态性 体尺性状 屠宰性能 肉质性状 |
| 收稿时间： | 2020-07-01 |
Correlations between MyoG gene polymorphism and body size,slaughter performance and meat traits of Yaoshan chickens |
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| YANG De-feng,LI Wei,LIU Yang,ZHANG Yun,GONG Yu,ZHANG Lan-yi,CHEN Ying,LIN Jia-dong,ZHANG Fu-ping,TANG Ji-gao.Correlations between MyoG gene polymorphism and body size,slaughter performance and meat traits of Yaoshan chickens[J].Journal of Southern Agriculture,2021,52(10):2887-2895. |
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| Authors: | YANG De-feng LI Wei LIU Yang ZHANG Yun GONG Yu ZHANG Lan-yi CHEN Ying LIN Jia-dong ZHANG Fu-ping TANG Ji-gao |
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| Abstract: | 【Objective】To explore the relevance of myogenin(MyoG) gene polymorphism to body size, slaughter performance and meat traits of Yaoshan chickens. Breeding of Yaoshan chickens with regard to a molecular marker would better protect, develop and utilize Yaoshan chickens, and at the same time provide a reference for MyoG gene research in other chicken breeds.【Method】The experiment involved 150-day-old healthy Yaoshan chickens, using PCR product direct sequencing technology on the Yaoshan chicken MyoG gene. Polymorphisms were detected, and DNAStar was used to compare and analyze the sequencing results to determine the SNP site of the MyoG gene. SPSS 18.0 was used to analyze the correlations between SNP locus andgrowth, slaughter performance and meat quality, and RNAfold was used to predict the mRNA secondary structure before and after MyoG gene mutation in Yaoshan chickens.【Result】One SNP(T17C locus) was found in the third exon of the MyoG gene in Yaoshan chickens. It was a synonymous mutation, and there were three genotypes(CC, CT, TT);T was the dominant allele, and CT was the dominant genotype. The effective allele number(Ne) was close to 2.00, and the polymorphic information content(PIC) was 0.35, showing moderate polymorphism (0.250.05). The mutation at the T17C site of the MyoG gene changes its mRNA secondary structure, which in turn causes its minimum free energy to change from -452.60 kcal/mol to -465.24 kcal/mol. The results of correlation analysis among growth, slaughter and meat quality traits of different genotypes of Yaoshan chickens showed that most of the correlation analysis results among growth, slaughter and meat quality traits of the three genotypes showed the same trend. Among them, chest width and chest depth showed a very significant positive correlation in different genotypes of Yaoshan chickens(P<0.01).【Conclusion】There are three genotypes at the T17C locus of the MyoG gene in Yaoshan chickens, which are synonymous mutations and do not cause significant changes in the structure and function of amino acids and their proteins. However, the polymorphism has animpact on body size, slaughter traits and meat quality of Yaoshan chickens, so it can be used as a candidate genetic marker for molecular breeding of Yaoshan chickens. |
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