| 一株抗南方根结线虫长形赖氨酸芽孢杆菌的分离鉴定及应用 |
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| 引用本文： | 程菊娥,王东伟,成飞雪,朱春晖,王忠勇,史晓斌,郑立敏,苏品,刘勇,张德咏.一株抗南方根结线虫长形赖氨酸芽孢杆菌的分离鉴定及应用[J].南方农业学报,2021,52(10):2765-2775. |
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| 作者姓名： | 程菊娥 王东伟 成飞雪 朱春晖 王忠勇 史晓斌 郑立敏 苏品 刘勇 张德咏 |
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| 作者单位： | 1 湖南农业大学植物保护学院, 长沙 410128;2 湖南省农业科学院植物保护研究所, 长沙 410125;3 湖南杂交水稻研究中心, 长沙 410125 |
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| 基金项目： | 国家现代农业产业技术体系建设专项（CARS-16-E17）；国家自然科学基金项目（31401797，31871941） |
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| 摘 要： | 【目的】筛选对南方根结线虫具有良好活性的生防菌株，为杀线虫制剂的研制与应用提供理论依据。【方法】采用平板稀释法对从抗线虫黄瓜根系附近采集的土壤样品进行芽孢杆菌分离，通过浸渍法筛选出杀线活性高且稳定的菌株，结合形态特征、生理生化特性和16S rDNA序列分析对活性菌株进行分类鉴定，并通过离体、盆栽及田间试验对分离菌株防治南方根结线虫的效果进行验证。【结果】筛选出1株具有较高杀线虫活性的芽孢杆菌C1，经鉴定菌株C1为长形赖氨酸芽孢杆菌（Lysinibacillus macroides）。杀线活性测定结果表明，菌株C1发酵上清液对南方根结线虫卵孵化的相对抑制率为74.50%、对雌虫孵化的相对抑制率为95.17%、对2龄幼虫的24和48 h校正死亡率分别为98.83%和100.00%，且杀虫活性稳定；盆栽试验发现，菌株C1发酵液50倍稀释液对番茄南方根结线虫的防效为73.24%；田间防病试验结果显示，菌株C1发酵液50倍稀释液对黄瓜南方根结线虫病的防效达82.67%，且产量较清水对照增产20.0%。【结论】菌株C1不仅对南方根结线虫病具有良好的防治效果，且能促进作物生长，可作为生物农药和肥料在农业生产中开发利用。
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| 关 键 词： | 南方根结线虫 长形赖氨酸芽孢杆菌 鉴定 杀线活性 促生效果 |
| 收稿时间： | 2021-01-22 |
Isolation,identification and application of a strain of Lysinibacillus macrolides against Meloidogyne incognita |
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| CHENG Ju-e,WANG Dong-wei,CHENG Fei-xue,ZHU Chun-hui,WANG Zhong-yong,SHI Xiao-bin,ZHENG Li-min,SU Pin,LIU Yong,ZHANG De-yong.Isolation,identification and application of a strain of Lysinibacillus macrolides against Meloidogyne incognita[J].Journal of Southern Agriculture,2021,52(10):2765-2775. |
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| Authors: | CHENG Ju-e WANG Dong-wei CHENG Fei-xue ZHU Chun-hui WANG Zhong-yong SHI Xiao-bin ZHENG Li-min SU Pin LIU Yong ZHANG De-yong |
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| Affiliation: | 1 College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;2 Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China;3 Hunan Hybrid Rice Research Center, Changsha 410125, China |
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| Abstract: | 【Objective】To screen the biocontrol strains with good activity against Meloidogyne incognita and provide a theoretical basis for the development and application of nematicidal agents.【Method】Bacillus was isolated from soil samples collected near the root of cucumber resistant to M. incognita by plate dilution method. Strains with high and stable nematicidal activity were screened by impregnation method. The active strains were classified and identified based on morphological characteristics, physiology and biochemistry and 16S rDNA identification. In vivo, pot and field experiments were conducted to verify the effect of the isolated strain on the control of M. incognita.【Result】A Bacillus C1 with high nematicidal activity was screened, and the strain C1 was identified as Lysinibacillus macrolides. The nematicidal activity results showed that the relative inhibition rate of the fermentation supernatant of strain C1 on the egg hatching of M. incognita was 74.50%, the relative inhibition rate on the female hatching was 95.17%, and the 24 and 48 h corrected mortality of the second-stage juvenile were 98.83% and 100.00%, respectively, with stable nematicidal activity. Pot experiment showed that the control effect of 50 times dilution of fermentation broth of strain C1 on tomato M. incognita was 73.24%. The results of field disease control experiment showed that the control effect of 50 times dilution of C1 fermentation broth on cucumber M. incognita was 82.67%, with yield 20.0% higher than that of clear water control.【Conclusion】Strain C1 not only shows good control effect on M. incognita, but also promotes crop growth. It can be developed and utilized as biological pesticide and fertilizer in agricultural production. |
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