| 低温胁迫对葛藤幼苗生长发育及生理特性的影响 |
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| 引用本文： | 赵文武,黄莉娟,赵丽丽,王雷挺,谢文辉.低温胁迫对葛藤幼苗生长发育及生理特性的影响[J].南方农业学报,2022,53(11):3068-3078. |
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| 作者姓名： | 赵文武 黄莉娟 赵丽丽 王雷挺 谢文辉 |
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| 作者单位： | 贵州大学动物科学学院, 贵州贵阳 550025 |
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| 基金项目： | 国家自然科学基金项目(32060391)贵州省科技计划项目(黔科合支撑[2021]一般155,黔科合平台人才[2021]5636,黔科合基础[2020]1Z026) |
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| 摘 要： | 【目的】探明低温对葛藤幼苗生长发育和生理生化指标的影响,为葛藤种质耐低温鉴定及合理选育抗冷害性强的种质提供参考依据。【方法】以葛藤种质为试验材料,采用盆栽土培试验,在光照培养箱预培养一周后,以昼/夜25℃/20℃为对照(CK),设2个温度梯度进行低温处理,分别为轻度低温胁迫(记为LD,昼/夜15℃/10℃)和重度低温胁迫(记为SD,昼/夜10℃/5℃),测定不同处理葛藤幼苗的生长状况和生理生化指标,再结合相关性、主成分分析和隶属函数进行综合评价。【结果】随温度下降,葛藤幼苗的生长速度减缓,叶片变小,水含量下降,相对电导率上升。可溶性糖、脯氨酸(Pro)等渗透调节物质含量上升;丙二醛(MDA)含量上升但差异不显著(P>0.05);过氧化物酶(POD)、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)等保护酶活性上升,过氧化氢酶(CAT)活性下降,但始终保持在较高水平;内源激素中脱落酸(ABA)、赤霉素(GA3)和玉米素(ZT)含量均呈先升高后降低,生长素(IAA)含量呈下降趋势。相关分析结果表明,葛藤幼苗生长速度与MDA含量呈显著(P<0.05,下同)负相关,ABA含量与可溶性糖含量和相对电导率分别呈显著和极显著(P<0.01)负相关,POD含量与IAA含量呈显著正相关。根据特征值将指标分为6个主成分,结合隶属函数分析发现低温会对葛藤生长带来负面影响。【结论】低温对葛藤的生长有不良影响,但葛藤幼苗可通过增强渗透调节能力、提高保护酶活性、增加生长抑制类内源激素含量、降低生长促进类激素含量以抵御低温胁迫带来的不利影响。
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| 关 键 词： | 葛藤 低温胁迫 生理生化 内源激素 耐低温鉴定 |
| 收稿时间： | 2022-07-04 |
Effects of low temperature stress on growth and development and physiological characteristics of Pueraria lobata seedlings |
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| ZHAO Wen-wu,HUANG Li-juan,ZHAO Li-li,WANG Lei-ting,XIE Wen-hui.Effects of low temperature stress on growth and development and physiological characteristics of Pueraria lobata seedlings[J].Journal of Southern Agriculture,2022,53(11):3068-3078. |
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| Authors: | ZHAO Wen-wu HUANG Li-juan ZHAO Li-li WANG Lei-ting XIE Wen-hui |
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| Affiliation: | College of Animal Science, Guizhou University, Guiyang, Guizhou 550025, China |
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| Abstract: | 【Objective】To investigate the effects of low temperature on the growth and development of Pueraria lobata seedlings,physiological and biochemical indicators,and to provide a reference basis for the identification of low temperature tolerance of P. lobat germplasm and the rational selection of germplasm with strong resistance to cold damage.【Method】The germplasm of P. lobata was used as the experimental material in a potted soil culture test. After a week of pre-cultivation in a light incubator,day/night 25 ℃/20 ℃ was set as control(CK),two temperature gradients were set for low temperature treatment,which were mild low temperature stress(LD,day/night 15 ℃/10 ℃) and severe low temperature stress(SD,day/night 10 ℃/5 ℃).【Result】With the decrease of temperature,the growth rate of P. lobata seedlings slowed down,the leaves became smaller,the water content decreased and the relative conductivity increased. The content of soluble sugars,proline(Pro) and other osmoregulatory substances increased;malonaldehyde(MDA) content increased but the difference was not significant(P>0.05) ;the activities of protective enzymes such as peroxidase(POD),superoxide dismutase(SOD) and ascorbate peroxidase(APX) increased and catalase(CAT) activity showed a decreasing trend but always remained at a high level;the content of endogenous hormones such as abscisic acid(ABA),gibberellin(GA3) and zeaxanthin(ZT) showed an increasing trend and then decreased;the content of auxin(IAA) showed a decreasing trend.The correlation analysis indicated that growth rate was significantly(P<0.05,the same below) negatively correlated with MDA content;ABA content was significantly negatively correlated with relative conductivity,and soluble sugar content was highly significantly(P<0.01) negatively correlated with relative conductivity;POD content was significantly positively correlated with IAA content. According to the characteristic values,the indexes was divided into 6 principal components. Combined with the membership function analysis,it was found that low temperature would bring negative effects on the growth of P. lobat.【Conclusion】Low temperature has adverse effects on the growth of P. lobata,but P. lobata seedlings resist the adverse effects of low temperature stress by enhancing osmoregulation,increasing protective enzyme activity,increasing the content of growth-inhibiting endogenous hormones,and decreasing the content of growth-promoting hormones in vivo. |
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