| 金耳栽培基质物质降解与转化规律研究 |
| |
| 引用本文： | 曹瑶,李荣春,杨林雷,李梦杰,罗祥英,沈真辉,陆青青.金耳栽培基质物质降解与转化规律研究[J].南方农业学报,2021,52(9):2516-2523. |
| |
| 作者姓名： | 曹瑶 李荣春 杨林雷 李梦杰 罗祥英 沈真辉 陆青青 |
| |
| 作者单位： | 1 云南农业大学食用菌研究所, 昆明 650201;2 云南菌视界生物科技有限公司, 昆明 650200 |
| |
| 基金项目： | 国家自然科学基金—云南联合项目（U1802231） |
| |
| 摘 要： | 【目的】探索金耳不同生长发育时间栽培基质内物质降解与转化的变化规律，了解金耳对碳源的降解利用特点，为栽培培养基配方设计提供理论基础。【方法】测定金耳8个生长时间点未接种（0 d），以及接种后20、25、30、35、40、45和50 d]栽培基质中含水量、pH、培养料湿重及干重、子实体鲜重及干重、木质纤维素组分的降解量，分析不同生长阶段内各指标的变化情况。【结果】金耳生长发育周期内，含水量由60.27%下降至39.00%。接种后25 d pH由5.50降至4.55，之后pH基本稳定在4.20左右，总体为酸性条件。培养料干重由最初的735.00 g/袋降至530.00g/袋，子实体鲜重产量为644.71 g/袋。总培养基失重率27.89%，其中，绝对生物学效率12.33%，呼吸作用消耗率15.56%。30~35 d为子实体生长第1次高峰期，40~45 d为子实体生长第2次高峰期。0~40 d呼吸作用随着金耳子实体生长不断增加。木质纤维素组分的吸收利用顺序依次为纤维素、木质素和半纤维素，其利用率分别为33.90%、32.79%和30.76%。生长前期（0~30 d）主要以非木质纤维素类物质为碳源，生长后期（30~50 d）主要以木质纤维素类物质为碳源；整个生长周期内所需的44.72%的碳源来自木质纤维素，55.28%的碳源来自非木质纤维素类物质。【结论】金耳生长发育周期内对非木质纤维素（淀粉、蛋白质等）类物质的利用率高于木质纤维素类物质，可从此方面考虑进行培养基配方优化，以提高生物学效率。
|
| 关 键 词： | 金耳 碳源 木质纤维素 绝对生物学效率 |
| 收稿时间： | 2021-01-05 |
Regulation on the degradation and transformation of Naematelia aurantialba (Bandoni & M. Zang)Millanes & Wedinmedium |
| |
| CAO Yao,LI Rong-chun,YANG Lin-lei,LI Meng-jie,LUO Xiang-ying,SHEN Zhen-hui,LU Qing-qing.Regulation on the degradation and transformation of Naematelia aurantialba (Bandoni & M. Zang)Millanes & Wedinmedium[J].Journal of Southern Agriculture,2021,52(9):2516-2523. |
| |
| Authors: | CAO Yao LI Rong-chun YANG Lin-lei LI Meng-jie LUO Xiang-ying SHEN Zhen-hui LU Qing-qing |
| |
| Affiliation: | 1 Institute of Edible Fungi, Yunnan Agricultural University, Kunming 650201, China;2 Yunnan Junshijie Biotechnology Co., Ltd., Kunming 650200, China |
| |
| Abstract: | 【Objective】 To explore the changes in the degradation and transformation of the medium in different times of growth and development of Naematelia aurantialba(Bandoni & M. Zang)Millanes & Wedin,and to understand the degradation and utilization characteristics of the N. aurantialba for carbon sources. Provided a theoretical basis for the formulation of medium.【Method】 The changes in water content,pH,the wet weight and dry weight of the medium,fresh weight of fruitingbody and the dry weight,degradation of lignocellulose were measured in 8 times of growth【0 d(not inoculated,20,25,30,35,40,45,50 d after vaccination】 of the medium,and the changes of various indicators in different growth stages were analyzed.【Result】 In the cycle of N. aurantialba growth and development,water content decreased from 60.27% to 39.00%. At 25 d pH was reduced from 5.50 to 4.55,after which pH was basically stable at about 4.20,with overall acidic conditions. The dry weight of medium from the initial 735.00 g/bag to 530.00 g/bag,the fresh weight of fruitingbody production was 644.71 g/bag. The total medium reduction was 27.89%,of which the biological efficiency was 12.33%,and the absolute respiratory consumption was 15.56%. 30 to 35 d was the first peak of fruitingbody growth,and 40 to 45 d was the second peak of fruiting body growth. In the 0-40 d of N. aurantialba growth and development, respiratory effect with the growth of fruiting body continued to increase. The use of the lignocellulose of the medium parts was in order of cellulose,lignin,hemicellulose andthe utilization rates were 33.90%,32.79% and 30.76%. The early stage(0 to 30 d)was mainly carbon source of non-lignocellulose substances,and the later stage(30 to 50 d)of growth was mainly carbon source of lignocellulose substances,of which 44.72% of the carbon sources required came from lignocellulose and 55.28% of carbon sources came from non-lignocellulose.【Conclusion】 During the growth and development cycle of N. aurantialba,the utilization rate of non-lignocellulose(starch,protein)substances is proximity to that of lignocellulose. The optimization of medium formulation can be considered from this aspect,in the hope of improving biological efficiency. |
| |
| Keywords: | |
|
| 点击此处可从《南方农业学报》浏览原始摘要信息 |
|
点击此处可从《南方农业学报》下载全文 |
|
