| 间作麦冬对茶园土壤理化性质及微生物群落结构的影响 |
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| 引用本文： | 李奇松,雷卫星,刘江西,朱捷文,史凌珊,蔡普默,张见明,贾小丽.间作麦冬对茶园土壤理化性质及微生物群落结构的影响[J].南方农业学报,2021,52(12):3366-3374. |
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| 作者姓名： | 李奇松 雷卫星 刘江西 朱捷文 史凌珊 蔡普默 张见明 贾小丽 |
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| 作者单位： | 武夷学院茶与食品学院,福建武夷山 354300;福建省农业生态过程与安全监控重点实验室/福建农林大学生命科学学院,福州 350002;武夷学院茶与食品学院,福建武夷山 354300 |
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| 基金项目： | 福建省自然科学基金项目（2020J05217）；中央引导地方科技发展项目（2019L3012）；福建省中青年教师教育科研项目（JAT190787）；福建省农业生态过程与安全监控重点实验室开放基金项目（NYST-2019-02） |
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| 摘 要： | 【目的】分析间作麦冬对春季茶园土壤养分、酶活性和微生物群落结构的影响，探讨不同种植模式条件下，茶园土壤环境、微生物群落间的互作机制，为茶园间作模式提供理论依据。【方法】以山地梯田茶园为研究对象，通过16S rDNA高通量测序技术等，分析茶树单作、茶树—麦冬间作及茶园空地土壤（CK）的土壤养分含量、土壤酶活性及细菌群落结构多样性与丰富度差异。【结果】相对于单作，间作麦冬能显著提高茶园土壤含水量与有机质、速效氮、速效磷和速效钾含量（P<0.05，下同），以及脲酶、蛋白酶、纤维素酶和酸性磷酸酶活性，而pH无明显变化。相对于单作和CK，间作麦冬对茶园土壤细菌群落的多样性和丰富度无明显影响，但群落结构发生显著变化；其土壤变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和芽单胞菌门（Gemmatimonadetes）的相对丰度显著提高，而绿弯菌门（Chloroflexi）的相对丰度显著降低。相关分析结果表明，变形菌门和放线菌门与土壤速效营养、有机质和土壤相关酶呈显著正相关；芽单胞菌门与速效氮、速效磷呈显著正相关；绿湾菌门与有机质呈显著负相关。LEfSe分析结果表明，变形菌门中的根瘤菌目（Rhizobiales）、黄单胞菌目（Xanthomonadales）、罗丹诺杆菌科（Rhodanobacteraceae）、褚氏杆菌属（Chujaibacter）、伯克氏菌科未分类菌属（unidentified_Burkholderiaceae），以及放线菌门中的放线菌门未分类菌纲（unidentified_ Actinobacteria）为间作茶园土壤样本的标志性微生物，且相对丰度显著高于CK和单作处理。【结论】茶园间作麦冬可诱导土壤细菌群落结构发生变化，能富集更多参与土壤营养循环和拮抗病原菌的有益细菌，有利于提高土壤养分供给能力和茶树的抗病性。
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| 关 键 词： | 间作 细菌多样性 微生物群落结构 茶园 高通量测序 |
| 收稿时间： | 2021-09-01 |
Effects of intercropping Ophiopogon japonicus into tea plantation on its soil physicochemical properties and microbial community structure |
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| LI Qi-song,LEI Wei-xing,LIU Jiang-xi,ZHU Jie-wen,SHI Ling-shan,CAI Pu-mo,ZHANG Jian-ming,JIA Xiao-li.Effects of intercropping Ophiopogon japonicus into tea plantation on its soil physicochemical properties and microbial community structure[J].Journal of Southern Agriculture,2021,52(12):3366-3374. |
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| Authors: | LI Qi-song LEI Wei-xing LIU Jiang-xi ZHU Jie-wen SHI Ling-shan CAI Pu-mo ZHANG Jian-ming JIA Xiao-li |
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| Affiliation: | 1 College of Tea and Food Science, Wuyi University, Wuyishan, Fujian 354300, China;2 Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring/College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | 【Objective】The effects of Ophiopogon japonicus intercropping with tea plantation in springon soil nutrient，enzyme activity and microbial community structure were analyzed to study the interaction mechanism between soil environment and microorganism under different culture mode conditions，and provide theoretical basis for intercropping practice of tea plantation.【Method】The tea plantation located at mountain terrace was used as the research objective to analyze the nutrient content，enzyme activity，bacterial community structure and diversity in soilunder tea monoculture，teaO. japonicusi intercropping and tea plantation soil（CK）by using 16S rDNA high-throughput sequencing technology.【Result】Compared to monoculture，O. japonicusi intercropping significantly increased soil water content，organic matter， available nitrogen（N），available phosphorus（P），available potassium（K），urease，protease，cellulase and acid phosphatase（P<0.05，the same below），while pH was not evidently changed. The diversity and richness of soil bacterial community in tea plantation were not greatly influenced by intercropping as compared with that of monoculture and CK，whereas the community structure was significantly changed. The relative abundances of Proteobacteria，Actinobacteria and Gemmatimonadetes were significantly increased in the intercropping treatment，while Chloroflexi was significantly decreased. The correlation analysis showed that Proteobacteria and Actinomycetes were significantly positively correlated with the soil available nutrients，soil organic matter and enzymes；Gemmatimonadetes were significantly positively correlated with available N and available P；Chloroflexi had negative correlation with soil organic matter. LEfSe analysis results showed that Rhizobiales，Xanthomonadales，Rhodanobacteraceae，Chujaibacter and unidentified Burkholderiaceae from Proteobacteria，and unidentified Actinobacteria from Actinobacteria were the biomark microorganisms in soil of intercropping tea plantation，and their abundance were significantly higher than that of CK and monoculture treatment.【Conclusion】 Intercropping O. japonicusi in tea plantation can alter the community structure of soil bacteria and enrich more beneficial bacteria that involve in nutrient cycling and antagonistic pathogens，which is conducive to improve soil nutrient supply capacity and tea disease resistance. |
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