| 丁酸梭菌拌料投喂对凡纳滨对虾肠道上皮细胞及菌群结构的影响 |
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| 引用本文： | 杨铿,蒋魁,洪敏娜,胡晓娟,陈海谊,徐创文,冷加华,曹煜成,文国樑.丁酸梭菌拌料投喂对凡纳滨对虾肠道上皮细胞及菌群结构的影响[J].南方农业学报,2022,53(12):3576-3583. |
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| 作者姓名： | 杨铿 蒋魁 洪敏娜 胡晓娟 陈海谊 徐创文 冷加华 曹煜成 文国樑 |
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| 作者单位： | 1 中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室, 广东广州 510300;2 广州市欣海利生生物科技有限公司, 广东广州 510300 |
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| 基金项目： | 国家重点研发计划项目（2020YFD0900401）；国家虾蟹产业技术体系建设专项（CARS-48）；广东省现代农业产业技术体系创新团队建设专项（2019KJ149）；中国水产科学研究院基本科研业务费专项（2020TD54） |
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| 摘 要： | 【目的】探究在高位池养殖凡纳滨对虾中添加丁酸梭菌对肠道上皮细胞及菌群结构的影响，为对虾健康养殖提供技术支持。【方法】设加菌组和对照组，饲料投喂频次为每天3餐，试验周期设为60 d。加菌组每天早上添加1%的丁酸梭菌拌料投喂，饲喂3 d后停3 d菌剂拌料投喂，以此为1个周期；对照组不添加丁酸梭菌。分别于0和60 d取样测定对虾体重和体长，并取对虾肠道进行组织切片和细菌群落结构分析。【结果】加菌组对虾的终体质量和终体长分别达9.54±0.38 g和9.12±0.12 cm，均显著高于对照组的7.35±0.36 g和8.25±0.10 cm （P<0.05，下同）。加菌组对虾的食物肠道饱满，肠道上色素呈稳定规则的星状结构，而对照组出现空肠现象，肠道上色素呈淡红色，且分散无固定的形态。加菌组对虾的肠道上皮细胞高度可达40 μm，显著高于对照组（10 μm）。在对虾肠道细菌群落结构方面，60 d时，加菌组厚壁菌门（Firmicutes）和拟杆菌门（Bacteroidetes）的相对丰度分别为50.44%和18.62%，显著高于对照组的0.05%和3.05%，而加菌组软壁菌门（Tenericutes）和变形菌门（Proteobacteria）的相对丰度分别为10.03%和15.69%，显著低于对照组的56.25%和38.23%。在属水平上，60 d时，加菌组梭菌属（Clostridium）和乳杆菌属（Lactobacillus）的相对丰度分别可达22.49%和26.45%，显著高于对照组的5.02%和0.03%，而弧菌属（Vibrio）和Candidatus Bacilloplasma的相对丰度分别为8.16%和9.18%，显著低于对照组的14.39%和55.81%。【结论】通过在饲料中添加1%丁酸梭菌可显著提升对虾生长性能，增加肠道上皮细胞高度，改善肠道菌群结构，促进肠道有益菌生长，抑制有害菌的繁殖。
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| 关 键 词： | 凡纳滨对虾 丁酸梭菌 肠道上皮细胞 菌群结构 |
| 收稿时间： | 2022-05-16 |
Effects of feedstuff contained Clostridium butyricum on intestinal epithelial cells and bacterial community structure in Litopenaeus vannamei |
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| YANG Keng,JIANG Kui,HONG Min-na,HU Xiao-juan,CHEN Hai-yi,XU Chuang-wen,LENG Jia-hua,CAO Yu-cheng,WEN Guo-liang.Effects of feedstuff contained Clostridium butyricum on intestinal epithelial cells and bacterial community structure in Litopenaeus vannamei[J].Journal of Southern Agriculture,2022,53(12):3576-3583. |
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| Authors: | YANG Keng JIANG Kui HONG Min-na HU Xiao-juan CHEN Hai-yi XU Chuang-wen LENG Jia-hua CAO Yu-cheng WEN Guo-liang |
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| Affiliation: | 1 South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou, Guangdong 510300, China;2 Guangzhou Xinhailisheng Biotechnology Co., Ltd., Guangzhou, Guangdong 510300, China |
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| Abstract: | 【Objective】 To study effects of feedstuff contained Clostridium butyricum on intestinal epithelial cells and bacterial community structure of Litopenaeus vannamei cultured in higher ponds, and to provide technical support for culture of healthy shrimp.【Method】 C. butyricum-added group and control group were set under feeding frequency of 3 meals per day in 60 d. In C. butyricum-added group, shrimps were fed with 1% C. butyricum every morning for 3 d and stopped for 3 d, which formed a circle;in control group, shrimps were was fed without C. butyricum. Shrimp body length and mass were measured on 0 and 60 d and intestines of shrimp were sliced to analyze bacterial community structure there.【Result】 Final body mass and body length of L. vannamei in the C. butyricum-added group were 9.54±0.38 g and 9.12±0.12 cm, respectively, significantly higher than those in the control group 7.35±0.36 g and 8.25±0.10 cm(P<0.05, the same below). In bacteria-added group, shrimp intestinal tract was full, with stable and regular star-like structure pigment;while in control group, shrimp intestinal tract was empty, where pigment was light red, scattered, without fixed shape. Height of intestinal epithelial cells in the bacteria-added group was up to 40 μm, significantly higher than that of intestinal epithelial cells in the control group(10 μm). In terms of bacterial community structure in shrimp intestinal tract, relative abundance of Firmicutes and Bacteroides on 60 d accounted for 50.44% and 18.62% in bacteria-added group, respectively, which were significantly higher than 0.05% and 3.05% in the control;those of Tenericutes and Proteobacteria accounted for 10.03% and 15.69%, respectively, significantly lower than 56.25% and 38.23% in the control group. At the genus level, relative abundance of Clostridium and Lactobacillus on 60 d in experimental group accounted for 22.49% and 26.45% respectively, significantly higher than 5.02% and 0.03% in the control; Vibrio and Candidatus Bacilloplasma in the experimental group reached 8.16% and 9.18%, respectively, significantly lower than 14.39% and 55.81% in the control.【Conclusion】 Adding 1% C. butyricum to feed can significantly improve the growth performance of L. vannamei, increase intestinal epithelial cell height, alter bacterial community structure in intestinal tract, thus making intestinal beneficial for bacteria growing better, and inhibit harmful bacteria. |
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