| 烟草种质资源TI203对烟草花叶病的抗性特点 |
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| 引用本文： | 于海芹,饶思莲,赵丹阳,刘勇,李永平.烟草种质资源TI203对烟草花叶病的抗性特点[J].南方农业学报,2017,48(11):2004-2009. |
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| 作者姓名： | 于海芹 饶思莲 赵丹阳 刘勇 李永平 |
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| 作者单位： | 云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明,650021云南省烟草公司 昭通市公司,云南 昭通,657000浙江大学 农业与生物技术学院 生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州,310058 |
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| 基金项目： | 中国烟草总公司科技项目，中国烟草总公司云南省公司科技项目 |
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| 摘 要： | 目的]系统了解烟草种质资源TI203抗烟草花叶病毒(Tobacco mosaic virus,TMV)的特点,为烟草抗病育种的亲本选择提供更丰富信息.方法]以烟草种质资源TI203为材料,采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法,研究TI203对TMV的抗性特异性、TI203接种TMV普通株系(TMV-U1株系)后的症状、病毒复制水平等.结果]TI203只对TMV表现无症状的抗病特点,接种马铃薯Y病毒(PVY)和黄瓜花叶病毒(CMV)则表现出典型症状.接种TMV-U1株系后14 d,Western blotting检测结果显示,TI203叶片病毒复制水平明显低于对照品种;用表达GFP的TMV侵染性克隆浸润接种发现,TI203对TMV的抗性发生在病毒复制水平.在高温和强日照条件下,TI203生长迅速的中上部叶片会发生日灼现象,且难以恢复.结论]TI203只对TMV表现无症状,其对TMV的抗性发生在病毒复制水平.
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| 关 键 词： | 烟草种质 TI203 烟草花叶病毒(TMV) 抗病性 |
Resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus |
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| YU Hai-qin,RAO Si-lian,ZHAO Dan-yang,LIU Yong,LI Yong-ping.Resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus[J].Journal of Southern Agriculture,2017,48(11):2004-2009. |
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| Authors: | YU Hai-qin RAO Si-lian ZHAO Dan-yang LIU Yong LI Yong-ping |
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| Abstract: | Objective]This research was conducted to understand the resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus(TMV),in order to provide more information for parent selection in disease resis-tant breeding of tobacco.Method]Tobacco germplasm resource TI203 was taken as experimental material. The resistance specificity of TI203 to TMV,and symptom and level of virus replication of TI203 after inoculated with TMV ordinary strain(TMV-U1 strain)were studied by TMV inoculation,disease investigation,virus coat protein accumulation detec-tion,TMV replication level determination and observation on sunburn phenomenon.Result]The results showed that TI203 exhibited a symptomless reaction to TMV,but typical symptoms to Potato virus Y(PVY)and Cucumber mosic vi-rus(CMV)after inoculation. Western blotting detection results indicated that the level of virus replication in TI203 leaves was obviously lower than that of control variety 14 d after inoculated with TMV-U1 strain. Resistance of TI203 to TMV was observed at virus replication level by using TMV-GFP infectious clone infiltration inoculation. Under high tempera-ture and strong sunlight conditions,sunburn phenomena always occurred on fast-growing middle and upper leaves of TI203,and the leaves were difficult to recover.Conclusion]TI203 is symptomless to TMV and its resistance to TMV occurs at virus replication level. |
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| Keywords: | tobacco germplasm TI203 Tobacco mosaic virus(TMV) disease resistance |
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