| 柠檬酵素对肥胖小鼠降脂减肥及免疫功能的调节作用 |
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| 引用本文： | 韩杰,王璐瑶,冯瑞章,梁启明,梁寒峭,魏琴,张勇波.柠檬酵素对肥胖小鼠降脂减肥及免疫功能的调节作用[J].南方农业学报,2022,53(2):557-567. |
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| 作者姓名： | 韩杰 王璐瑶 冯瑞章 梁启明 梁寒峭 魏琴 张勇波 |
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| 作者单位： | 1 宜宾学院生命科学与食品工程学院/香料植物资源开发与利用四川省高校重点实验室, 四川宜宾 644000;2 北京城市学院生物医药学部, 北京 100083;3 宜宾市龙茂柠檬种植有限公司, 四川宜宾 644000 |
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| 基金项目： | 国家自然科学基金;香料植物资源开发与利用四川省高校重点实验室开放基金项目 |
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| 摘 要： | 【目的】明确柠檬酵素对小鼠降脂减肥作用及免疫功能的影响，为探究柠檬酵素用于治疗肥胖引起的高血压、糖尿病和心血管疾病提供理论依据。【方法】以SPF级雄性C57BL/6J小鼠为研究对象，通过饲喂H10301型60%高脂高糖饲料35 d构建肥胖模型，然后以低剂量（310.0 mg/kg）、中剂量（620.0 mg/kg）、高剂量（1240.0 mg/kg）柠檬酵素及奥利司他（阳性对照，15.6 mg/kg）分别经口灌胃肥胖小鼠，持续给药42 d，空白对照组小鼠饲喂普通饲料及灌胃等体积的生理盐水。给药期间记录动物体重及摄食量；给药结束后计算小鼠的Lee’ s指数、脏器指数及脂体比，测定各处理组小鼠的血脂水平［总甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）］及葡萄糖耐量，制作组织切片观察肝脏脂质沉积情况和病理变化，并通过流式细胞术检测小鼠脾脏淋巴细胞各亚群比例。【结果】经柠檬酵素处理后可有效降低肥胖小鼠的体重、Lee’ s指数和脂体比，其中以柠檬酵素高剂量组的效果最佳，表现为小鼠的体重和Lee’ s指数极显著低于肥胖模型组小鼠（P<0.01，下同），脂体比显著低于肥胖模型组小鼠（P<0.05，下同），但对小鼠的摄食量无显著影响（P>0.05，下同）。灌胃柠檬酵素后，肥胖小鼠的肝脏指数明显下降，而脾脏、心脏和肾脏的脏器指数均无显著差异；血清中的TC、TG和LDL-C水平较肥胖模型组小鼠呈不同程度的下降趋势，HDL-C水平则极显著升高；柠檬酵素可改善肥胖小鼠肝脏中脂质聚积的情况，其葡萄糖耐受能力也得到明显改善。此外，柠檬酵素能促进肥胖小鼠脾脏中CD4+比例升高，同时降低CD8+比例，且具有一定的浓度依赖性，在高剂量柠檬酵素作用下CD4+/CD8+比值由肥胖模型组小鼠的1.49±0.11上升至3.20±0.42； CD4+Foxp3+在柠檬酵素介入后显著降低。【结论】柠檬酵素对高脂饲料诱导肥胖小鼠具有较强的减肥效果，还能改善肥胖小鼠免疫状态较差的现象，因此柠檬酵素可作为减肥保健食品在慢性疾病辅助治疗中推广应用。
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| 关 键 词： | 小鼠 柠檬酵素 肥胖症 脂质沉积 葡萄糖耐量 淋巴细胞亚群 |
| 收稿时间： | 2021-01-24 |
Beneficial effects of lemon enzyme on high-fat diet-induced obesity and immune function in mice |
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| HAN Jie,WANG Lu-yao,FENG Rui-zhang,LIANG Qi-ming,LIANG Han-qiao,WEI Qin,ZHANG Yong-bo.Beneficial effects of lemon enzyme on high-fat diet-induced obesity and immune function in mice[J].Journal of Southern Agriculture,2022,53(2):557-567. |
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| Authors: | HAN Jie WANG Lu-yao FENG Rui-zhang LIANG Qi-ming LIANG Han-qiao WEI Qin ZHANG Yong-bo |
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| Affiliation: | 1 College of Life Sciences&Food Engineering, Yibin University/Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin, Sichuan 644000, China;2 Dean of Biomedical School, Beijing City University, Beijing 100094, China;3 Yibin Longmao Lemon Plantation Company, Yibin, Sichuan 644000, China |
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| Abstract: | 【Objective】To illustrate the effect of lemon enzyme on reducing fat,weight loss and immune function in mice,so as to provide a theoretical basis for the treatment of hypertension,diabetes and cardiovascular diseases caused by obesity by lemon enzyme.【Method】C57BL/6J mice of SPF grade were fed H10301, a 60% high-fat and high sugar diet for 35 days to establish the obesity model. Then,the mice of the fat model group were gavaged with either a low concentration(310.0 mg/kg),medium concentration(620.0 mg/kg) or high concentration(1240.0 mg/kg) of citrin for 42 days. Orlistat(15.6 mg/kg) was used as a positive control. The mice of the control group were fed with normal diet and normal saline of the same volume. The body weight and food intake were recorded during administration. After administration, the Lee's index,organ index and lipid body ratio were calculatedand the blood lipid levels total triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C) and glucose tolerance of mice in each treatment group were measured. Tissue sections were prepared to observe the changes in liver lipid deposition and pathological structure and the proportion of lymphocyte subsets in the mouse spleen were detected by flow cytometry.【Result】Fat model groups receiving a lemon enzyme treatmenthad significantly lowered body weight, Lee's index and fat body ratio,of which the treatment with the high concentration of lemon enzyme produced the best improvements:relative to the control group,the body weight and Lee's index of mice were notably and significantly lower (P<0.01,the same below) and the fat body ratio was significantly lower(P<0.05,the same below),with no significant effect on mice food intake(P>0.05,the same below). After intragastric administration of lemon enzyme,the liver index of obese mice decreased significantly,but there was no significant difference in the organ indices of spleen,heart orkidney. The levels of serum TC,TG and LDL-C were decreased in varying degrees compared with the control group,while the level of serum HDL-C increased significantly. Lemon enzyme reduced lipid accumulation in the liver of obese mice, with an improved glucose tolerance. In addition,lemon enzyme increased the proportion of CD4+ in the spleen of obese mice and reduced the proportion of CD8+. After treatment with the high concentration lemon enzyme,the ratio of CD4+/CD8+ changed from 1.49±0.11 to 3.20±0.42 in the obese model group. CD4+Foxp3+ decreased significantly after lemon enzyme intervention.【Conclusion】Lemon enzyme can reduce weight induced by a high-fat diet and improve the poor immune state in obese mice. Therefore,lemon enzyme can be used as a weight loss health food in the adjuvant treatment of chronic diseases. |
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