| 蛴螬提取物对视网膜静脉阻塞兔内皮抑素表达的影响 |
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| 作者姓名： | 梁凯霞 蒋鹏飞 彭俊 彭清华 |
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| 作者单位： | 湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007,湖南中医药大学第一附属医院, 湖南 长沙 410007;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208 |
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| 基金项目： | 国家自然科学基金面上资助项目（30772824、81574031）；中医药防治五官科疾病湖南省重点实验室建设项目（2017TP1018）；长沙市科技计划项目（K1501014-31、kc1704005）；中央财政支持地方高校建设项目；国家中医药管理局中医眼科学重点学科建设项目；湖南省中医五官科学重点学科建设项目 |
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| 摘 要： | 目的 评价蛴螬提取物对兔视网膜静脉阻塞的治疗作用及其机制。方法 将40只兔随机分为4组：空白组、模型组、复方血栓通组、蛴螬组，每组10只兔20只眼，除空白组外，其余3组均用光化学动力法建立视网膜静脉阻塞（retinal vein occlusion，RVO）动物模型，于给药后1周、3周行FFA检查、HE染色、免疫组化观察内皮抑素表达。结果 FFA检查结果显示：给药后周1周，模型组中央静脉荧光渗漏、出血，复方血栓通组和蛴螬组渗漏、出血较模型组少；给药后3周，模型组渗漏、出血的静脉周围出现无灌注区，复方血栓通组渗漏、出血明显减小，蛴螬组渗漏、出血基本吸收。视网膜切片光镜观察结果显示：蛴螬组视网膜各层结构病理变化均轻于模型组、复方血栓通组。内皮抑素免疫组化结果显示：各造模组内皮抑素表达均下降，蛴螬组表达最强，模型组表达最弱，差异有统计学意义（P<0.05）。结论 蛴螬提取物能改善视网膜缺血缺氧的状态，促进RVO模型中内皮抑素的表达。
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| 关 键 词： | 视网膜静脉阻塞 蛴螬提取物 内皮抑素 |
| 收稿时间： | 2018/10/10 0:00:00 |
Effect of Grub Extract on the Expression of Endostatin in Rabbits with Retinal Vein Occlusion |
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| Authors: | LIANG Kaixi JIANG Pengfei PENG Jun and PENG Qinghua |
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| Affiliation: | Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China and Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China |
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| Abstract: | Objective To evaluate the therapeutic effect and mechanism of action of grub extract on rabbits with retinal vein occlusion (RVO). Methods Forty rabbits were randomly and equally divided into blank group, model group, compound Xueshuantong group, and grub group, with 20 eyes in each group. A rabbit model of RVO was established by a photochemical kinetic method in all groups except for the blank group. Fluorescein fundus angiography (FFA), HE staining, and immunohistochemistry (for measuring endostatin expression) were performed at 1 and 3 weeks after treatment. Results According to the FFA results, at 1 week after treatment, central venous fluorescence leakage and hemorrhage were observed in the model group; the compound Xueshuantong group and grub group had less leakage and hemorrhage than the model group. At 3 weeks after treatment, areas of non-perfusion showed up around the vein with leakage and hemorrhage in the model group; the compound Xueshuantong group had substantially reduced leakage and hemorrhage; the leakage and hemorrhage were almost absorbed in the grub group. The results of optical microscopic examination of retinal sections showed that the grub group had less pathological changes in each retina layer than the model group and the compound Xueshuantong group. According to the immunohistochemistry results, the expression of endostatin was significantly reduced in all three model establishment groups; among the three groups, the grub group had the highest expression of endostatin, while the model group had the lowest expression (P<0.05). Conclusion Grub extract can improve retinal ischemia and hypoxia and promote the expression of endostatin in the rabbit model of RVO. |
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| Keywords: | retinal vein occlusion grub extract endostatin |
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