| 心康冲剂改善慢性心衰模型大鼠心肌凋亡及调控Caspase-3/9的表达 |
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| 作者姓名： | 刘蓉芳 毛以林 谭雄 张辉 毛湘屏 杨柳 陈志成 |
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| 作者单位： | 江门市五邑中医院, 广东 江门 529000;湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005,湖南中医药大学第二附属医院, 湖南 长沙 410005 |
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| 基金项目： | 湖南省自然科学基金（2016JJ4068）；湖南省中医药科研计划（201610）；国家重点实验室中医诊断学开放基金（2015ZYZD11）；2016年研究生创新科研课题（2016CX08）。 |
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| 摘 要： | 目的 探讨心康冲剂对心衰大鼠Caspase-3、Caspase-9表达的调控作用。方法 58只SD大鼠分为正常组10只，模型组、心康组及对照组各16只，造模完后，心康组给予心康冲剂溶液0.5 g/kg灌服，对照组予芪苈强心胶囊溶液0.06 g/kg灌服。采用心脏彩超检测心功能；心脏切片行TUNNEL染色法观察心肌细胞凋亡；心肌组织采用Real-time PCR法及免疫组化法检测Caspase-3、Caspase-9基因及蛋白质表达。结果 与正常组比，模型组大鼠Caspase-3及Caspase-9的mRNA与蛋白表达显著增加（P<0.01），TUNNEL染色观察细胞凋亡明显增多；与模型组比较，心康组大鼠心肌凋亡减轻， Caspase-9 mRNA、Caspase-3 mRNA及蛋白明显下降（P<0.01）；与对照组相比，心康组心肌细胞凋亡相对降低，Caspase-3蛋白表达下降（P<0.05）。结论 心康冲剂可调控下调Caspase-3、Caspase-9表达抗心衰。
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| 关 键 词： | 心肌凋亡 Caspase-3 Caspase-9 慢性心衰 心康冲剂 |
| 收稿时间： | 2017/9/10 0:00:00 |
Xinkang Granule Improves Myocardial Apoptosis and the Expression of Caspase-3/9 in Rats with Chronic Heart Failure |
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| Authors: | LIU Rongfang MAO Yilin TAN Xiong ZHANG Hui MAO Xiangping YANG Liu and CHEN Zhicheng |
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| Affiliation: | Wuyi Hospital of Traditional Chinese Medicine, Jiangmen, Guangdong 529000, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China,The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China and The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China |
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| Abstract: | Objective To investigate the regulatory effects of Xinkang Granule (XKG) on the rats with chronic heart failure (CHF) for the expression of Caspase-3 and Caspase-9. Methods A total of 58 SD rats were randomly divided into a normal group (n=10), a model group, a XKG group, and a control group, with 16 rats in each group. After the success of the modeling, the XKG group was given XKG solution at 0.5 g/kg, and the control group was given Qili Qiangxin Capsule (QLQXC) solution at 0.06 g/kg. Cardiac function was measured by echocardiography; Heart slices were stained by TUNNEL to observe myocardial apoptosis. The expression of gene and protein of Caspase-3, Caspase-9 were detected by Real-time PCR and immunohistochemistry. Results Compared with the normal group, the gene and protein of Caspase-3, Caspase-9 in the model group significantly increased (P<0.01). TUNNEL observed significant increase of apoptosis. Compared with the model group, the rats in XKG group showed alleviated myocardial apoptosis, and the Caspase-3, Caspase-9 mRNA and protein expression were significantly reduced (P<0.01). Compared with the control group, the myocardial apoptosis in the XKG group was relatively decreased, and Caspase-3 decreased (P<0.05). Conclusion XKG has the function of anti-heart failure by regulating and controlling the expression of Caspase-3 and Caspase-9. |
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| Keywords: | myocardial fibrosis Caspase-3 Caspase-9 chronic heart failure Xinkang Granule |
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