| 表达中国小麦花叶病毒（CWMV）外壳蛋白基因增强烟草对CWMV的抗病性 |
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| 引用本文： | 杨锦,靳鹏,刘芃,羊健,王洋,戴良英,陈剑平.表达中国小麦花叶病毒（CWMV）外壳蛋白基因增强烟草对CWMV的抗病性[J].浙江农林大学学报,2020,37(2):291-295. |
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| 作者姓名： | 杨锦 靳鹏 刘芃 羊健 王洋 戴良英 陈剑平 |
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| 作者单位： | 1.湖南农业大学 植物保护学院, 湖南 长沙 4100002.宁波大学 植物病毒研究所, 浙江 宁波 3150003.浙江农林大学 农业与食品科学学院 浙江省农产品品质改良技术研究重点实验室, 浙江 杭州 311300 |
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| 基金项目： | 国家农业产业体系资助项目CARS-3-1国家小麦转基因专项2016ZX08002001 |
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| 摘 要： | 目的 中国小麦花叶病毒（Chinese wheat mosaic virus，CWMV）是引起小麦黄花叶病的重要病原之一。获得表达CWMV外壳蛋白（coat protein，CP）的转基因烟草Nicotiana benthamiana（OECP）并分析其抗病性，为培育小麦抗病材料奠定工作基础。 方法 利用体外重组DNA技术构建含有CWMV外壳蛋白基因的表达载体，并通过农杆菌介导的转化方法，获得OECP。进一步利用Western Blot和PCR检测明确CWMV的外壳蛋白在OECP中能够正常的表达。 结果 部分转基因阳性植株出现矮化表型。此外，OECP阳性植株接种CWMV后7 d的定量分析表明，OECP中CWMV运动蛋白的表达量显著受到抑制。 结论 在烟草中表达CWMV的CP蛋白基因可显著增强烟草对CWMV的抗病性。
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| 关 键 词： | 植物保护学 中国小麦花叶病毒（CWMV） 外壳蛋白 表达 烟草 抗病性 |
| 收稿时间： | 2019-03-27 |
Transgenetic expression coat protein of Chinese wheat mosaic virus(CWMV) enhances resistance of Nicotiana benthamiana to CWMV |
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| YANG Jin,JIN Peng,LIU Peng,YANG Jian,WANG Yang,DAI Liangying,CHEN Jianping.Transgenetic expression coat protein of Chinese wheat mosaic virus(CWMV) enhances resistance of Nicotiana benthamiana to CWMV[J].Journal of Zhejiang A&F University,2020,37(2):291-295. |
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| Authors: | YANG Jin JIN Peng LIU Peng YANG Jian WANG Yang DAI Liangying CHEN Jianping |
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| Affiliation: | 1.College of Plant Protection, Hunan Agricultural University, Changsha 410000, Hunan, China2.Institute of Plant Virology, Ningbo University, Ningbo 315000, Zhejiang, China3.The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China |
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| Abstract: | Objective Chinese wheat mosaic virus (CWMV) is an important virus causing wheat mosaic virus disease. The objective of this study is to obtain expression transgenic tobacco (OECP) of CWMV coat protein (CP) gene and analyze its disease resistance, which lays a foundation for the cultivation of wheat disease resistance materials. Method The expression vector containing the coat protein gene of CWMV was constructed by in vitro recombinant DNA technology and OECP was obtained by Agrobacterium-mediated transformation assay. Western Blot and PCR analysis showed that the CP gene of CWMV was correctly expressed in OECP. Result Some transgenic positive plants displayed dwarf phenotype. The quantitative analysis in OECP showed that expression level of CWMV motor protein gene was significantly reduced 7 days after inoculation with CWMV. Conclusion Expressing CP protein gene could significantly enhance resistance of Nicotiana benthamiana to CWMV. |
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