| 金线莲组培快繁技术 |
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| 引用本文： | 何云芳,杨霞,余有祥,裘丽珍,江胜德.金线莲组培快繁技术[J].浙江农林大学学报,1999,16(2):170-174. |
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| 作者姓名： | 何云芳 杨霞 余有祥 裘丽珍 江胜德 |
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| 作者单位： | 1.浙江省林业厅种苗站, 浙江杭州 310004;2.浙江省杭州市农业科学研究所, 浙江 杭州 310008 |
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| 摘 要： | 用金线莲的茎段作外植体, 比较不同的培养基、细胞分裂素、生长素、植物添加剂等因素对丛生芽生成的影响。实验证明B5 及MS 较好, BA 的作用优于KT和ZT , 最适质量浓度为4.0 mgL-1 ;NAA 0.3 mgL-1和GA3 0.3mgL-1对丛生芽的生成和伸长有较好的效果。比较不同的培养基、蔗糖质量浓度、生长素质量浓度和茎段放置方法对金线莲生根的影响。实验证明1/10 MS 的培养基, 20 gL-1的蔗糖质量浓度, NAA 0.5 mgL-1的生长素质量浓度生根效果较好;培养体放置方法以立于培养基表面为好。表6 参4
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| 关 键 词： | 金线莲 组织培养 快速繁殖 |
| 收稿时间： | 1998-05-12 |
Techniques of tissue culture and rapid propagation for Anoectochilus formosanus |
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| HE Yun-fang,YANG Xia,YU You-xiang,QIU Li-zhen,JIANG Sheng-de.Techniques of tissue culture and rapid propagation for Anoectochilus formosanus[J].Journal of Zhejiang A&F University,1999,16(2):170-174. |
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| Authors: | HE Yun-fang YANG Xia YU You-xiang QIU Li-zhen JIANG Sheng-de |
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| Affiliation: | 1.Seed and Seedling Station of Zhejiang , Hangzhou 310004, China;2.Agriculture Research Institute of Hangzhou, Hangzhou 310008, China |
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| Abstract: | The cluster shoots and roots induced from stem segments of Anoectochilus formosanus in tissue cultures was studied .The results showed that B5 and MS media , BA , NAA , GA , with the concentrations of 4.0 mgL-1 , 0.3 mgL-1 , 0.3 mgL-1 respectively , and LB were suitable for cluster buds generating and growing .1/10MS medium , 20 gL-1 sucrose and 0.5mgL-1NAA were suitable for roots , and the segments vertically put on the surface of the medium rooted better . |
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