| 铝胁迫对绣球组培苗生长及生理特性的影响 |
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| 引用本文： | 李叶华,陈爽,赵冰.铝胁迫对绣球组培苗生长及生理特性的影响[J].浙江农林大学学报,2020,37(6):1064-1070. |
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| 作者姓名： | 李叶华 陈爽 赵冰 |
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| 作者单位： | 西北农林科技大学 风景园林艺术学院，陕西 杨凌 712100 |
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| 基金项目： | 陕西省西安市科技计划项目(20NYYF0064) |
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| 摘 要： | 目的 探究蜡莲绣球Hydrangea strigosa和莼兰绣球Hydrangea longipes组培苗的耐铝能力，为抗铝植物的应用和酸性土壤特殊资源的利用奠定基础。 方法 以蜡莲绣球和莼兰绣球组培苗为试验材料，研究培养基中不同氯化铝质量浓度(0、25、50、100、200 mg·L?1)对绣球组培苗生长及生理特性的影响。 结果 较低质量浓度铝胁迫下(25、50 mg·L?1)，蜡莲绣球生长正常，长势与对照组相当，莼兰绣球长势不及对照组；中高质量浓度铝胁迫下(100、200 mg·L?1)，组培苗长势明显减弱，平均根长和根体积均低于对照组，表明铝胁迫对绣球的地上与地下部分均产生了抑制作用。随着铝胁迫质量浓度的增加，蜡莲绣球叶绿素质量分数在50 mg·L?1时有所增加，莼兰绣球叶绿素质量分数呈现不断下降趋势，表明蜡莲绣球在低质量浓度铝胁迫时促进了叶绿素合成，莼兰绣球在铝胁迫下叶绿素受损。蜡莲绣球与莼兰绣球叶片丙二醛质量摩尔浓度先下降后上升，过氧化氢酶和过氧化物酶活性不断增强，超氧化物歧化酶活性均低于对照组，但差异不显著。蜡莲绣球脯氨酸质量分数在氯化铝质量浓度25 mg·L?1处理下低于对照组，其余质量浓度处理下高于对照组，莼兰绣球铝胁迫处理组脯氨酸质量分数均高于对照组。 结论 绣球在铝胁迫下能作出积极的生长与生理响应，且不同种绣球的耐铝程度有差异。综合各指标分析，蜡莲绣球和莼兰绣球对低质量浓度的铝胁迫有一定的抗性。图2表3参24
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| 关 键 词： | 植物学 铝胁迫 蜡莲绣球 莼兰绣球 组培苗 生长特性 生理特性 |
| 收稿时间： | 2020-01-07 |
Effect of aluminum stress on growth and physiological characteristics of Hydrangea tissue culture seedlings |
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| LI Yehua,CHEN Shuang,ZHAO Bing.Effect of aluminum stress on growth and physiological characteristics of Hydrangea tissue culture seedlings[J].Journal of Zhejiang A&F University,2020,37(6):1064-1070. |
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| Authors: | LI Yehua CHEN Shuang ZHAO Bing |
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| Affiliation: | College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, Shaanxi, China |
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| Abstract: | Objective The aim is to explore the aluminum tolerance of Hydrogena strigosa and H. longipes tissue culture seedlings, so as to lay a foundation for the application of aluminum resistant plants and the utilization of special acid soil resources. Method The effects of different concentrations of AlCl3 (0, 25, 50, 100, 200 mg·L?1) on the growth and physiological characteristics of H. strigosa and H. longipes tissue culture seedlings were studied. Result Under low concentration of aluminum stress (25, 50 mg·L?1), the growth of H. strigosa was normal and similar to that of the control group, while the growth of H. longipes was inferior to that of the control group. Under the medium and high concentration of aluminum stress (100, 200 mg·L?1), the growth of tissue culture seedlings was significantly reduced, and the average root length and root volume were lower than those of the control group, indicating that aluminum stress inhibited both the aboveground and underground parts of Hydrangea. With the increase of aluminum stress concentration, the chlorophyll mass fraction of H. strigosa increased at 50 mg·L?1, while the chlorophyll mass fraction of H. longipes decreased, which indicated that H. strigosa promoted chlorophyll synthesis under low aluminum stress, and H. longipes inhibited chlorophyll synthesis under aluminum stress. The content of malondialdehyde in H. strigosa and H.longipes decreased first and then increased. The catalase and peroxid activities increased continuously. The activities of superoxide dismutase were lower than those of the control group, but the difference was not significant. The proline mass fraction of H. strigosa was lower than that of the control group under the treatment of 25 mg·L?1, and higher than that of the control group under other aluminum concentration treatment. The proline mass fraction of H. longipes under aluminum stress was higher than that of the control group. Conclusion Hydrangea can make positive growth and physiological responses under aluminum stress, and different species of Hydrangea have different aluminum tolerance. In sum, H. strigosa and H. longipes are resistant to low concentration of aluminum.Ch, 2 fig. 3 tab. 24 ref.] |
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