| 长喙毛茛泽泻根茎叶营养成分与急性经口毒性 |
| |
| 引用本文： | 石从广,李浩铭,仇建习,吕贤良,叶珊.长喙毛茛泽泻根茎叶营养成分与急性经口毒性[J].浙江农林大学学报,2021,38(6):1170-1177. |
| |
| 作者姓名： | 石从广 李浩铭 仇建习 吕贤良 叶珊 |
| |
| 作者单位： | 1.浙江省林业科学研究院，浙江 杭州3100232.丽水市莲都区生态林业发展中心，浙江 丽水323000 |
| |
| 基金项目： | 国家自然科学基金资助项目(31800539)；丽水市莲都区野外绝灭植物长喙毛茛泽泻抢救保护工程资助项目(2019-08) |
| |
| 摘 要： | 目的 长喙毛茛泽泻Ranalisma rostratum是极度濒危保护植物，被纳入国家Ⅰ级重点保护植物。近年来，全国先后建立了多个长喙毛茛泽泻繁育基地，其种群数量得到了有效提升。对长喙毛茛泽泻营养成分及急性经口毒性的研究，有利于对其进行更好的保护利用。 方法 选取丽水市3个回归示范点的4批次样品，烘干前后称量样品质量，计算各营养器官的生物量比和干鲜比，测定其茎叶根的主要营养成分和元素(包括脂肪、氮、磷、钾、钙、镁、铁、锰、锌)的质量分数以及茎叶的小鼠Mus musculus急性经口毒性。 结果 ①长喙毛茛泽泻植株的干鲜比为0.17~0.19，整个植株含水率较高。②长喙毛茛泽泻植株中8种元素的平均质量分数从高到低依次为氮、钾、铁、钙、磷、镁、锰、锌，铁质量分数在各样品和组织间变异系数最大，其次是磷和镁。③脂肪质量分数在4个样品之间存在显著差异(P＜0.05)，除锌外，其余7种元素在4个样品间均无显著差异；脂肪质量分数在长喙毛茛泽泻的茎叶和根之间没有显著差异，而钠、磷、钾、钙质量分数在茎叶和根间差异显著(P＜0.05)。④钠、钾、钙这3种元素两两之间存在显著(P＜0.05)或极显著(P＜0.01)正相关，而与磷存在显著或极显著负相关，磷与镁存在显著正相关。⑤以长喙毛茛泽泻茎叶制取悬浮样品液，喂饲试验显示:对雌雄小鼠经口半致死量均大于7.5 g·kg?1。 结论 长喙毛茛泽泻中的镁、铁和磷质量分数不够稳定，而其他元素均较稳定。钠、磷、钾、钙质量分数在长喙毛茛泽泻的茎叶和根系间的分布有显著差异。小鼠急性经口毒性试验结果显示:其茎叶属实际无毒。图1表5参28
|
| 关 键 词： | 植物学 长喙毛茛泽泻 营养元素 脂肪 急性经口毒性试验 |
| 收稿时间： | 2021-01-06 |
Nutritional components of rhizome and leaf of Ranalisma rostratum and its acute oral toxicity |
| |
| SHI Congguang,LI Haoming,QIU Jianxi,Lü Xianliang,YE Shan.Nutritional components of rhizome and leaf of Ranalisma rostratum and its acute oral toxicity[J].Journal of Zhejiang A&F University,2021,38(6):1170-1177. |
| |
| Authors: | SHI Congguang LI Haoming QIU Jianxi Lü Xianliang YE Shan |
| |
| Affiliation: | 1.Zhejiang Academy of Forestry, Hangzhou 310023, Zhejiang, China2.Liandu Ecological Forestry Development Center of Lishui City, Lishui 323000, Zhejiang, China |
| |
| Abstract: | Objective Ranalisma rostratum, a critically endangered plant species, is included in the national level Ⅰ key protection plants. Many breeding bases have been established in China in recent years, and the population of R. rostratum has been effectively increased. This study aims to explore the nutritional composition and acute oral toxicity of R. rostratum for its better protection and utilization. Method The 4 batches of samples from 3 regression plots in Lishui City were selected, and weighed before and after drying. The biomass ratio and dry-to-fresh ratio of each vegetative organ were calculated. The main nutrients and elements (including fat, N, P, K, Ca, Mg, Fe, Mn, Zn)in stems, leaves and roots, as well as the acute oral toxicity of stems and leaves in Mus musculus were determined. Result (1) The dry-to-fresh ratio of the plant was 0.17?0.19, indicating that the water content of the whole plant was high. (2) The average contents of 8 nutrient elements from high to low were N, K, Fe, Ca, P, Mg, Mn and Zn. The coefficient of variation of Fe content among samples and tissues was the largest, followed by P and Mg. (3) There was a significant difference in fat content among the 4 samples (P＜0.05). There were no significant differences in the other 7 elements except Zn among the 4 samples. There were no significant differences in fat content between stem, leaf and root, but a significant difference in Na, P, K and Ca between the stem, leaf and root (P＜0.05). (4) There was a significant (P＜0.05) or extremely significant (P＜0.01) positive correlation among Na, K and Ca, and the 3 elements had significant or extremely significant negative correlation with P, while P had significant positive correlation with Mg. (5) The stem leaf suspended sample liquid was prepared for the acute oral toxicity test, which showed that the oral LD50 of male and female mice was greater than 7.5 g·kg?1 body weight. Conclusion The mass fraction of Mg, Fe and P in R. rostratum was unstable, while other elements were stable. The distribution of Na, P, K and Ca in the stem, leaf and root of R. rostratum was significantly different. The acute oral toxicity test of stem and leaf in M. musculus showed that it was practically non-toxic. Ch, 1 fig. 5 tab. 28 ref.] |
| |
| Keywords: | |
|
| 点击此处可从《浙江农林大学学报》浏览原始摘要信息 |
|
点击此处可从《浙江农林大学学报》下载全文 |
|
