| 烟草赤星病生防菌的鉴定及其抑菌机理 |
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| 引用本文： | 陈 娜,陈 婧,闵琪茹,安德荣,文俊明,杨 照,王 阳.烟草赤星病生防菌的鉴定及其抑菌机理[J].西北农业学报,2023(8):1268-1278. |
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| 作者姓名： | 陈 娜 陈 婧 闵琪茹 安德荣 文俊明 杨 照 王 阳 |
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| 作者单位： | (1.西北农林科技大学 植物保护学院，陕西杨凌 712100；2.陕西省烟草公司咸阳市公司，陕西咸阳 712000) |
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| 基金项目： | 陕西省烟草公司咸阳市公司科技项目(［2021］46号)。 |
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| 摘 要： | 由交替链格孢（Alternaria alternata）引起的烟草赤星病是烟草生长中后期重要的叶部病害，直接影响烟叶的产量和品质。为开发烟草赤星病的生防制剂，对生防菌XNB-01进行了多相分类鉴定，并通过盆栽试验、粗提物的抑菌机理等研究，进一步阐明了XNB-01的生防机理。皿内对峙结果表明，XNB-01对交替链格孢的抑菌半径达到 11.0 mm。菌株XNB-01短杆状，与Paenibacillus polymyxa ATTCC 842相似性为98.63%。次生代谢产物检测结果表明，XNB-01可以分泌多种真菌细胞壁降解酶，并具有溶解有机磷、解钾的能力。盆栽试验结果表明，XNB-01在 1×1048 CFU/mL的施用浓度下显著增加了烟草幼苗的株高、鲜质量和干质量；在1×109 CFU/mL的施用浓度下显著降低了烟草赤星病的病情指数，防效为60.94%，与菌核净（65.33%）相当。此外，XNB-01 的胞内粗提物可抑制交替链格孢菌丝的生长和孢子萌发，使菌丝膨大，孢子皱缩。经200 μg/mL XNB-01 胞内粗提物处理后，峰值处的胞外电导率、核酸和蛋白质泄漏量分别是对照组的2.97、1.83、8.28倍，丙二醛含量比对照升高1.36倍。综上所述，P.polymyxa XNB-01 具有防治烟草赤星病的生防潜力。
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| 关 键 词： | 烟草赤星病 多粘类芽孢杆菌 抑菌机理 生物防治 |
Identification and Antifungal Mechanism of Biocontrol Bacteria against Tobacco Brown Spot |
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| CHEN N,CHEN Jing,MIN Qiru,AN Derong,WEN Junming,YANG Zhao and WANG Yang.Identification and Antifungal Mechanism of Biocontrol Bacteria against Tobacco Brown Spot[J].Acta Agriculturae Boreali-occidentalis Sinica,2023(8):1268-1278. |
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| Authors: | CHEN N CHEN Jing MIN Qiru AN Derong WEN Junming YANG Zhao and WANG Yang |
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| Abstract: | Tobacco brown spot, caused by Alternaria alternata (Fr.) Keissl, is a highly detrimental fungal disease that significantly affects the quantity of tobacco.In order to screen effective candidate biocontrol agents against A.alternata, the XNB-01 was classified and identified using polyphasic classification techniques, and the biological control mechanism of XNB-01was further elucidated by pot-experiment and crude extract inhibition test.The plate confrontation test showed that XNB-01 exhibited inhibitory activity against A.alternata, forming an inhibition zone with a radius of 11.0 mm.Strain XNB-01 was identified as a short rod-shaped bacterium, sharing 98.63% similarity with Paenibacillus polymyxa ATTCC 842 .The secondary metabolite assay showed that XNB-01 produced various extracellular hydrolytic enzymes, and exhibited the organic phosphorus and potassium solubilization abilities.In the pot-experiment, treatment with XNB-01 at the application concentration of 1×108 CFU/mL significantly increased the tobacco seedling growth in terms of plant height, fresh mass and dry mass, treatment with XNB-01 at 1×109 CFU/mL significantly reduced the disease index of tobacco brown spot, and the biocontrol efficacy was 60.94%, comparable to that of myclobutanil (65.33%).The crude extract inhibited the mycelial growth and spores germination of A.alternata and changed the morphology of spores and mycelium.The crude extracts of XNB-01 resulted in a 2.97-fold increase in extracellular conductivity, a 1.83-fold and 8.28-fold increase in nucleic acid and protein leakage, and a 1.36-fold increase in malondialdehyde content in A.alternata compared to the control group.To sum up, P.polymyxa XNB-01 exhibits promising biocontrol potential for management of tobacco brown spot disease. |
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| Keywords: | Tobacco brown spot Paenibacillus polymyxa Antifungal Biological control |
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