角果木内生真菌活性分子对HeLa细胞增殖凋亡的影响 |
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引用本文: | 孙梦宇,周董董,邓勤,徐静.角果木内生真菌活性分子对HeLa细胞增殖凋亡的影响[J].热带生物学报,2020,11(4):399-405. |
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作者姓名: | 孙梦宇 周董董 邓勤 徐静 |
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作者单位: | 1.海南大学 化学工程与技术学院,海口 570228 |
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基金项目: | 海南省研究生创新科研项目;海南省教育厅重点项目;高层次人才项目;国家自然科学基金 |
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摘 要: | 为了解红树林植物角果木(Ceriops tagal)内生壳囊孢属真菌Cytospora sp.提取物WP-1[(22E, 24R)-5, 8-epidioxy-5α, 8α-ergosta-6, 9(11), 22E-trien-3β-ol]抑制人宫颈癌HeLa细胞的效果,笔者采用MTT比色法、克隆形成实验、Hoechst 33258染色和蛋白免疫印迹法来研究WP-1对HeLa细胞增殖和凋亡的影响。结果表明,随着WP-1浓度增加,HeLa细胞的存活率显著降低,而且该提取物能明显抑制HeLa细胞的克隆形成,当其浓度达到10 μmol·L?1时,HeLa细胞出现染色质浓缩以及核小体碎裂的凋亡现象。蛋白免疫印迹法结果显示,随着WP-1剂量的增加,与凋亡相关的蛋白Caspase-3及Caspase-9的表达下调,Cleaved Caspase-3,Cleaved Caspase-9的表达明显上调。本实验结果显示,WP-1可以抑制HeLa细胞的增殖,并促进HeLa细胞发生凋亡。
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关 键 词: | 角果木 Cytospora sp. (22E 24R)-5 8-epidioxy-5α 8α-ergosta-6 9(11) 22E-trien-3β-ol HeLa细胞 增殖 凋亡 |
收稿时间: | 2020-05-18 |
Effects of Bio-active Molecules Isolated from Endophytic Fungi of Ceriops tagal on the Proliferation and Apoptosis of HeLa Cells |
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Affiliation: | 1.School of Chemical Engineering and Technology, Hainan University, Haikou, Hainan 5702282.College of Horticulture, Hainan University, Haikou, Hainan 570228 |
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Abstract: | In order to understand the effect of WP-1 (22E, 24R)-5, 8-epidioxy-5α, 8α-ergosta-6, 9(11), 22E-trien-3β-ol] isolated from Cytospora sp., an endophytic fungus from the mangrove plant Ceriops tagal on human cervical cancer HeLa cells, the proliferation and apoptosis of HeLa cells treated with WP-1 was determined and analyzed by using MTT colorimetry, clone formation, Hoechst 33258 staining and Western blot. It was found that with the increase of WP-1 concentration the survival rate of the HeLa cells decreased significantly, and that the WP-1 significantly inhibited the colony formation of cells. When treated with WP-1 at a concentration of 10 μmol·L?1 the HeLa cells showed chromatin condensation and nucleosome fragmentation, which were characteristic of apoptosis. Western blot analysis showed that with the increase of WP-1 dose, the expressions of apoptosis-related proteins Caspase-3 and Caspase-9 were down-regulated in the HeLa cells, while the expressions of Cleaved Caspase-3 and Cleaved Caspase-9 were significantly up-regulated. The results show that WP-1 can inhibit the proliferation of HeLa cells and promote apoptosis of HeLa cells. |
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