| 海巴戟果提取物对凡纳滨对虾副溶血性弧菌的抑制效应 |
| |
| 引用本文： | 常逢彤,冯谦洁,黎娜,周海龙.海巴戟果提取物对凡纳滨对虾副溶血性弧菌的抑制效应[J].热带生物学报,2021,12(1):1-6. |
| |
| 作者姓名： | 常逢彤 冯谦洁 黎娜 周海龙 |
| |
| 作者单位： | 海南大学 热带生物资源教育部重点实验室/生命科学与药学院 海口 570228 |
| |
| 基金项目： | 海南省基础与应用基础研究计划（自然科学领域高层次人才项目，2019RC077） |
| |
| 摘 要： | 采用传统煎煮法提取海巴戟果有效成分，以副溶血性弧菌为试验菌株，将抑菌圈直径、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）及抑菌率作为检测指标，探讨海巴戟果热水提取物对水产动物致病菌的体外抑菌效果。结果表明，海巴戟果提取物对副溶血性弧菌的生长有抑制作用，其最小抑菌质量浓度（MIC）为25.00 g·L?1，最小杀菌质量浓度（MBC）为50.00 g·L?1。海巴戟果提取物对副溶血性弧菌的抑菌效果随着紫外照射时长而减弱，甚至消失，煮沸时间≥15 min才能使其更好地发挥抑菌作用。
|
| 关 键 词： | 海巴戟 提取物 凡纳滨对虾 副溶血性弧菌 抑菌效果 |
| 收稿时间： | 2020-10-08 |
Bacteriostatic Effect of Noni (Morinda citrifolia) Fruit Extract on Vibrio parahaemolyticus Isolated from Litopenaeus vannamei |
| |
| Affiliation: | Hainan University; Ministry of Education Key Laboratory of Tropical Biological Resources/ School of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan 570228，China |
| |
| Abstract: | The effective components of noni (Morinda citrifolia) fruit were extracted by traditional water decocting method and used to test their bacteriostatic effect on Vibrio parahaemolyticus isolated from Litopenaeus vannamei. V. parahaemolyticus is the causative agent of acute hepatopancreatic necrosis disease that causes serious loss in aquaculture of Litopenaeus vannamei. Diameter of inhibition zones, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bacteriostatic rate were determined, based on which the antibacterial effects of the noni fruit extract on V. parahaemolyticus in vitro were discussed. The results showed that the noni fruit extract inhibited the growth of V. parahaemolyticus at the minimum inhibitory concentration (MIC) of 25.00 g·L?1 and the minimum bactericidal concentration (MBC) of 50.00 g·L?1. The bacteriostatic effect of the noni fruit extract on V. parahaemolyticus decreased or even disappeared with the increase of duration of exposure of the noni fruit extract to ultraviolet. Only when boiled for 15 min or more for extraction could M. citrifolia have a better bacteriostatic effect on the growth of V. parahaemolyticus. |
| |
| Keywords: | |
|
| 点击此处可从《热带生物学报》浏览原始摘要信息 |
|
点击此处可从《热带生物学报》下载全文 |
