The Extraction,Isolation and Identification of Exudates from the Roots of Flaveria bidentis |
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| Authors: | YANG Xing ZHANG Li-hui SHI Cui-ping SHANG Yan ZHANG Jin-lin HAN Jian-min DONG |
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| Affiliation: | Molecular Plant Pathology Laboratory, College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China |
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| Abstract: | Large amounts of Flaveria bidentis's root culturing solution were obtained by using DFT (deep flow technique) equipment and these solution which was vacuum concentrated (10, 20 mg mL−1) can have a certain inhibition on Triticum aestivum, Cucumis sativus, Raphanus sativus, Amaranthus retroflexus, Setaria viridis, Chenopodium album, Echinochloa crusgalli and Chloris virgata. This outcome suggested some active compounds in the root exudates of Flaveria bidentis can inhibit the germination, seedling elongation and root length. The dichloromethane extract of root exudates was identificated by GC-MS, and 29 kinds of compounds, including esters, hydrocarbons, ketones, thiazole, amines, etc. were obtained and the phthalate n-octyl ester, phthalate 2-ethylhexyl ester were proved to be allelochemicals. The culturing solution of root exudates was separated through the resin column and silica gel column and a component inhibiting seedling height, root length and fresh weight of wheat was got. There were 6 kinds of organic compounds in this component including dioctyl phthalate, 1,2-phthalate, mono(2-ethylhexyl) ester by GC-MS. |
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| Keywords: | extraction isolation identification exudates |
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