Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936 |
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| Authors: | Jin-gang LIANG Dan-dan ZHANG Dong-yang LI Sheng-yuan ZHAO Chen-yao WANG Yu-tao XIAO Dong XU Yi-zhong YANG Guo-ping LI Li-li WANG Yu GAO Xue-qing YANG Hai-bin YUAN Jian LIU Xiu-jie ZHANG Kong-ming WU |
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| Affiliation: | 1. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, P.R.China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;3. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China;4. Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China;5. Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China;6. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P.R.China;7. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China;8. Yantai Academy of Agricultural Sciences, Yantai 265500, P.R.China;9. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China;10. College of Plant Protection, Jilin Agricultural University, Changchun 130118, P.R.China;11. College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China |
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| Abstract: | The fall armyworm (FAW) Spodoptera frugiperda, which originated in the Americas, is advancing across China and threatening the nation’s maize crops. Currently, one widely used tool for its control is genetically modified (GM) Bacillus thuringiensis (Bt) maize. Sufficient content of Bt protein in appropriate plant parts is crucial for enhancing resistance against insect pests. In this study, we conducted a systematic investigation of Cry1Ab levels in Chinese domestic GM maize DBN9936, which has recently obtained a biosafety certificate, and evaluated its efficacy against FAW. Quantification of expression levels of Cry1Ab, via ELISA, indicated a spatio-temporal dynamic, with significant variation of mean Cry1Ab, ranging from 0.76 to 8.48 μg g–1 FW with the Cry1Ab protein level ranked as: V6–V8 leaf>R1 leaf>R4 leaf>R1 silk>VT tassel>R4 kernel. Among the nine locations, the Cry1Ab levels in DBN9936 of the Xinxiang, Langfang, and Harbin fields were significantly lower than those from Wuhan and Shenyang, and were slightly, but not significantly lower than those from the other four fields. Furthermore, the artificial diet–Cry1Ab mixture and plant tissue feeding bioassays revealed that DBN9936 has high efficacy against FAW. The insecticidal efficacy of different tissues against FAW larvae reached 34–100% with a descending order of lethality as follows: VT leaf>R4 leaf>R1 husk>R1 silk>VT tassel>R4 kernel. Taken together, our results showed that Bt-Cry1Ab maize DBN9936 has potential as a promising strategy to manage FAW.
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| Keywords: | fall armyworm genetically modified maize DBN9936 Cry1Ab expression control efficacy |
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