Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| |
| Authors: | LI Fu-hua ZHENG Shao-jie ZHAO Ji-chun LIAO Xia WU Su-rui MING Jian |
| |
| Affiliation: | 1 College of Food Science, Southwest University, Chongqing 400715, P.R.China
2 Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.China
3 Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooperatives, Kunming 650223, P.R.China |
| |
| Abstract: | Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties. However, both its biological effects and the possible mechanism of action have not yet been known. We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck (MPE) against HepG2 human hepatocellular carcinoma cells. Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90% (P<0.01). The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax, Bcl-2 and cleaved caspase-3. On the other hand, MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21, Cyclin D1, cyclin-dependent kinases-4 (CDK4) and proliferating cell nuclear antigen (PCNA). Additionally, MPE downregulated TRAF-2 and p-p53, while upregulated p-ASK1 and p-p38. Therefore, it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway. |
| |
| Keywords: | phenolic extract cell cycle apoptosis anti-proliferation |
| 本文献已被 ScienceDirect 等数据库收录！ |
| 点击此处可从《农业科学学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《农业科学学报(英文版)》下载全文 |
|
