| 复方黄连乳腺炎注射液的药理作用与安全性研究 |
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| 引用本文： | 郝满良,白丽欣,秦建华.复方黄连乳腺炎注射液的药理作用与安全性研究[J].中国农学通报,2012,28(11):55-60. |
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| 作者姓名： | 郝满良 白丽欣 秦建华 |
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| 作者单位： | 河北农业大学动物科技学院,河北保定,071001 |
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| 基金项目： | 河北省教育厅2009年科研计划项目“复方黄连奶牛乳腺炎注射液的研制”（2009453）; 国家‘十一五’重大专项子专题“华北农区奶牛疾病综合防治技术应用与示范”（2006BAD04A10—4） |
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| 摘 要： | 为了探讨复方黄连乳腺炎注射液的药理作用及安全性,为兽医临床用药提供理论依据。采用小鼠耳廓肿胀、毛细血管通透性、和棉球诱发小鼠肉芽组织增生方法研究了复方黄连乳腺炎注射液的抗炎作用;采用小鼠腹腔巨噬细胞吞噬鸡红细胞、小鼠吞噬细胞碳粒廓清能力、以及胸腺指数、脾脏指数研究了该注射液的免疫作用;采用改进Karber法,以半数致死量（LD50）作为评价急性毒性强度的指标,研究了该注射液的急性毒性,并对该注射液的热源性、刺激性进行了检测。结果表明：复方黄连乳腺炎注射液高、中剂量组对小鼠耳廓的肿胀度分别为1.24、1.78mg,与对照组（生理盐水）的肿胀度（2.94mg）相比差异极显著（P〈0.01）;对毛细血管通透性的OD值,高、中、低剂量组分别为0.05、0.14、0.14,与对照组（生理盐水）的OD值（0.21）相比差异极显著（P〈0.01）;对小鼠肉芽组织增重,高、中、低剂量组分别为10.18、8.10、10.16mg,与对照组肉芽组织增重（11.90mg）相比差异极显著（P〈0.01）;高、中、低剂量组对小鼠胸腺指数、脾指数的增加有极显著性差异（P〈0.01）;高、中剂量组对小鼠腹腔巨噬细胞吞噬红细胞能力与对照组比较具有极显著性差异（P〈0.01）;高剂量组对小鼠吞噬细胞碳粒廓清能力与对照组相比差异极显著（P〈0.01）,中剂量、低剂量与对照组相比差异不显著;急性毒性试验LD50为11222.77mg/kg。数据表明复方黄连乳腺炎注射液对急性和慢性炎症有显著的抗炎作用,能增强机体的免疫功能,且安全可靠。
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| 关 键 词： | 复方黄连乳腺炎注射液 抗炎试验 免疫试验 急性毒性试验 热源性检测 |
| 收稿时间： | 2011/10/19 0:00:00 |
| 修稿时间： | 2/5/2012 12:00:00 AM |
Research on Pharmacological Functions and Safety of Compound Rhizoma coptidis Injection on Mastitis |
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| Hao Manliang
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Bai Lixin
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Qin Jianhua.Research on Pharmacological Functions and Safety of Compound Rhizoma coptidis Injection on Mastitis[J].Chinese Agricultural Science Bulletin,2012,28(11):55-60. |
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| Authors: | Hao Manliang Bai Lixin Qin Jianhua |
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| Affiliation: | (College of Animal Science and Technology,Agricultural University of Hebei,Baoding Hebei 071001) |
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| Abstract: | In order to discuss the pharmacological activities and safety of the compound Rhizoma coptidis injection,provide theory basis for clinical medication.Using test methods of the mice＇s auricle inflammation,capillary vessel permeability and mice＇s granulation tissue by absorbent cotton,investigated anti-inflammatory effect of the compound Rhizoma coptidis injection on mastitis.Adopting the methods of mice＇s immune organ index,abdominal macrophage cell engulf chicken＇s red blood cell （RBC）,ability of the mice＇s phagocytic cell clearing carbon particles,studied immune function of the compound Rhizoma coptidis injection on mastitis.Using the ameliorate Karber and with half lethal dose （LD 50） as evaluation acute toxicity intensity index,studied the injection＇s acute toxicity,and tested the heat sources,excitant of this injection.The results showed that mice＇s auricle inflammation swelling degree of the compound coptis mastitis in jection＇s high,middle dose groups respectively are 1.24 mg,1.78 mg,and compared extremely significant difference （P0.01） with control group （physiological saline） swelling degree （2.94 mg）.For capillary permeability＇s OD value,high,medium and low doses groups respectively is 0.05,0.14,0.14,and compared extremely significant difference （P0.01） with control group （physiological saline） OD value （0.21）.In mice granulation tissue weight gain,high,medium and low doses groups respectively is 10.18 mg,8.10 mg,10.16 mg,and compared extremely significant difference （P0.01） with control group granulation tissue weight gain （11.90 mg）.In the high,medium and low dose groups,mice thymus index,spleen index increased with extremely significant difference （P0.01） compared with control group.In high,medium dose groups,the ability of macrophage in mice＇s abdominal cavity engulfed RBC compared with controls group have extremely significant difference （P0.01）.In high-dosage group,the ability of mice phagocytic cells cleared of carbon particles compared with control group extremely significant difference （P0.01）,but medium,low dose no significant difference compared with control group.Acute toxicity tests LD 50 for 11222.77 mg/kg.The data showed the compound rhizoma coptidis mastitis injection have significant anti-inflammatory effects on treatment acute and chronic inflammation,could increased the mice body＇s immune function,and safe reliable. |
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| Keywords: | compound Rhizoma coptidis injection anti-inflammatory experiments immune experiments acute toxicity tests heat source tests |
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