| 欧李增殖培养基的优化研究 |
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| 引用本文： | 孙晓丽,贾艳芳,高仪,王红霞,张志华.欧李增殖培养基的优化研究[J].中国农学通报,2013,29(31):118-122. |
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| 作者姓名： | 孙晓丽 贾艳芳 高仪 王红霞 张志华 |
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| 作者单位： | 1. 河北农业大学山区研究所2. |
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| 基金项目： | 河北省科学计划项目“欧李新品种选育与种质资源创新”（06220117D-3） |
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| 摘 要： | 筛选出适合欧李增殖的最适培养基,可以提高试管苗的质量和优化欧李无性繁殖体系。以欧李2号增殖培养阶段试管苗为试材,以不同培养基、不同激素浓度、不同蔗糖浓度、不同pH为主要研究内容,进行了增殖效果研究。MS培养基最适宜欧李2号的增殖;NAA 0.2 mg/L＋6-BA 0.5 mg/L＋MS处理,其平均分化新梢数和大于2 cm新梢数最多;MS培养基中蔗糖浓度40 g/L处理,既可显著提高分化新梢数又可提高成苗率;pH 6.0的MS培养基最适宜欧李2号生长。适于欧李2号的增殖培养基为MS培养基＋NAA0.2 mg/L＋6-BA0.5 mg/L＋6 g/L琼脂＋40 g/L蔗糖,pH 6.0。
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| 关 键 词： | 欧李 试管苗 增殖 影响因素 |
| 收稿时间： | 2013/3/11 0:00:00 |
| 修稿时间： | 2013/5/20 0:00:00 |
The optimized research on proliferation culture medium of Chinese Dwarf Cherry |
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| Sun Xiaoli,Jia Yanfang,Gao Yi,Wang Hongxia,Zhang Zhihua.The optimized research on proliferation culture medium of Chinese Dwarf Cherry[J].Chinese Agricultural Science Bulletin,2013,29(31):118-122. |
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| Authors: | Sun Xiaoli Jia Yanfang Gao Yi Wang Hongxia Zhang Zhihua |
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| Affiliation: | 1Mountainous Areas Research Institute, Research Center for Agricultural Engineering Technology of Mountain District, Agricultural University of Hebei, Baoding Hebei 071000; 2College of Horticulture, Agricultural University of Hebei, Baoding Hebei 071000) |
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| Abstract: | The most suitable medium for Cerasus humilis proliferation was selected to improve the quality of the plantlets and optimize asexual reproduction system of Cerasus humilis. The proliferation culture stage planflets of Chinese dwarf cherry 2 were as test materials, and different media, different hormone concentrations and different sucrose concentrations and different pH were as the main content to study the proliferation effect. The most appropriate medium for Cerasus humilis 2 to proliferation was MS. In the treatment of NAA 0.2 mg/L ＋6-BA 0.5 mg/L＋MS, the average differentiated shoots number and the number of shoots greater than 2 cm were the largest. The treatment of MS medium with sucrose concentration of 40 g/L could not only significantly increase the number of differentiated shoots but also improve seedling rate. The MS medium with pH 6.0 was most suitable for the growth of Cerasus humilis 2. Suitable for Prunus Humilis 2 proliferation medium was MS ＋NAA 0.2 mg/L＋6-BA 0.5 mg/L＋6 g/L agar ＋40 g/L sucrose, pH 6.0. |
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| Keywords: | influencing factors |
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