| 玉米自交系愈伤组织的诱导、RAPD分析及扫描电镜观察 |
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| 引用本文： | 杜娟,常国权,季静,王罡.玉米自交系愈伤组织的诱导、RAPD分析及扫描电镜观察[J].作物学报,2002,28(2):282-285. |
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| 作者姓名： | 杜娟 常国权 季静 王罡 |
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| 作者单位： | 中国人民解放军军需大学植物基因工程研究中心,吉林长春,130062 |
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| 基金项目： | 国家植物转基因中试及产业化基地”专项课题 (项目编号 J99-B-0 0 1) |
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| 摘 要： | 以玉米不同自交系的幼胚为材料,用诱导培养基进行培养,以获得愈伤组织.结果发现所有自交系幼胚均可诱导出白色或黄白色的愈伤组织,但其再生能力差异显著,这主要取决于基因型和培养基种类.自交系7922、340幼胚的再生频率高达85.5%和78%.植株再生途径也有所不同,8902、Mo17、4112和846以器官发生途径为主,340、7922、921、853和
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| 关 键 词： | 玉米自交系 组织培养 再生植株 RAPD 扫描电镜 |
| 收稿时间： | 2000-12-04 |
| 修稿时间： | 2000年12月4日 |
Observation with Scanning Electron Microscope and Analysis of RAPD on Tissue Culture and Embryogenesis of Inbreds of Zea mays L. |
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| DU Juan,CHANG Guo,Quan,JI Jing,WANG Gang.Observation with Scanning Electron Microscope and Analysis of RAPD on Tissue Culture and Embryogenesis of Inbreds of Zea mays L.[J].Acta Agronomica Sinica,2002,28(2):282-285. |
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| Authors: | DU Juan CHANG Guo Quan JI Jing WANG Gang |
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| Abstract: | Immature embryos of different maize inbreds were cultured in induced medium. All types formed pale yellow to yellow callus on the medium. Results indicated that plant regeneration was chiefly controlled by genotypes and kinds of medium. The rate of regeneration for inbreds 7922 and 340 was 85.5% and 78% respectively. There are different ways in the regeneration, and 8902, Mo17, 4112 and 846 tended to regenerate via organogensis, while 340, 7922, 921, 853 and 835 were likely to regeneration via somatic embryogenesis. Some calli lost their capacity of embryogenesis during long time subculture. The results of randomly amplified polymorphic DNA (RAPD) showed that the genomic DNA was modified in tissue culture. Two patterns of maize somatic embryogenesis were found by observation with scanning electron microscope. One was induced directly from immature embryo, and the other produced embryoids after subculturing several times. |
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| Keywords: | Maize Inbred line Tissue culture Plant regeneration RAPD Electron microscope |
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