| 烤烟成熟过程中叶片和叶绿体的细胞学观察 |
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| 引用本文： | 黄勇,周冀衡,杨虹琦,刘建利,王勇,周义和,黄琰,何小力.烤烟成熟过程中叶片和叶绿体的细胞学观察[J].作物学报,2006,32(11):1767-1769. |
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| 作者姓名： | 黄勇 周冀衡 杨虹琦 刘建利 王勇 周义和 黄琰 何小力 |
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| 作者单位： | 1湖南农业大学生物科学技术学院，烟草科学与工程重点实验室；2园艺园林学院，湖南长沙410128；3中国烟叶生产购销公司，北京100055；4常德卷烟厂技术中心，湖南常德415000 |
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| 基金项目： | 国家烟草专卖局资助项目;湖南农业大学校科研和教改项目 |
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| 摘 要： | 分别在烤烟K326（Nicotiana tabacum）中部第11叶的未熟、适熟、过熟时期，打孔取样。采用鲜样徒手切片观察不同成熟度叶片的解剖结构，并用酶解法游离出单个叶肉细胞，及撕取下表皮观察气孔保卫细胞，测定其中叶绿体数量和叶绿素含量。研究了烤烟成熟过程中叶片组织、细胞结构及叶绿体在形态和数量上的变化规律。发
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| 关 键 词： | 烤烟 叶片结构 气孔保卫细胞 叶绿体 |
| 收稿时间： | 2005-11-09 |
| 修稿时间： | 2006-03-14 |
Cytological Observation of Leaf and Chloroplast during the Flue-Cured Tobacco Maturation |
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| HUANG Yong,ZHOU Ji-Heng,YANG Hong-Qi,LIU Jian-Li,WANG Yong,ZHOU Yi-He,HUANG Yan,HE Xiao-Li.Cytological Observation of Leaf and Chloroplast during the Flue-Cured Tobacco Maturation[J].Acta Agronomica Sinica,2006,32(11):1767-1769. |
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| Authors: | HUANG Yong ZHOU Ji-Heng YANG Hong-Qi LIU Jian-Li WANG Yong ZHOU Yi-He HUANG Yan HE Xiao-Li |
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| Affiliation: | 1.Biology Science and Technology Department，Tobacco Science and Engineer Key Laboratory;2.Horticulture and gardens college, Huanan Agricultural University, Changsha 410128; Hunan;3.China Tobacco Purchase and Sale Company, Beijing 100055;4.Changde Quanyan Company Technology Center, Changde 415000, Hunan, China |
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| Abstract: | Flue-cured Tobacco K326(Nicotiana tabacum) was cultured in the experimental station of Hunan Agricultural University.The leaves were sampled when the middle leaf(the 11~(th) leaf) was green,ripe and aged.The leaves were sliced up by hand and observed.In order to get single cell,the leaves were treated with cellulase and pectinase.Epidermis was removed from the abaxial surface of a leaf,and the number of chloroplasts in the guard cells was counted.All the observations above were recoded with a camera under the optical microscope.The results showed that the leaf structure was loosen,the cell outline was gradually misty,the number of chloroplasts in chlorenchyma cells and guard cells decreased,the number of starch grains increased,and the number of chloroplasts and the chlorophyll content pen gram leaf decreased during the ripening and aging of the tobacco leaves. |
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| Keywords: | Flue-cured tobacco Lamina structure Stomata guard cell Chloroplast |
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