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普通小麦籽粒黄色素含量的QTL分析
引用本文:张立平,阎俊,夏先春,何中虎.普通小麦籽粒黄色素含量的QTL分析[J].作物学报,2006,32(1):41-45.
作者姓名:张立平  阎俊  夏先春  何中虎
作者单位:中国农业科学院作物科学研究所/国家小麦改良中心,北京100081
基金项目:引进国际先进农业科技计划(948计划);科技部科研项目
摘    要:小麦面粉黄色度b*值是反映面粉颜色的重要指标,主要与籽粒黄色素含量有关。利用122对SSR引物、4对贮藏蛋白STS引物和10对AFLP引物组合,分析了中优9507´CA9632的71个DH系,构建了由173个位点组成的遗传连锁图,在小麦21个连锁群上覆盖2 881 cM。将该群体种植2年共计5个地点,测定籽粒黄色素和面粉黄色度b*值含量。采用复合区间作图法(CIM)进行了籽粒黄色素含量和面粉黄色度QTL分析。结果表明,面粉黄色度b*值的QTL位于染色体1DS、2DL、3A、4D、5D、6AL、6D和7AL上,其中7AL的QTL效应最大,贡献率为12.9%~37.6%;籽粒黄色素含量的QTL位于染色体2DL、3DL、4A、5A和7AL,其中7AL的QTL效应最大,贡献率为12.1%~33.9%。面粉黄色度b*值与籽粒黄色素含量共同的QTL位于7AL,与Xgwm264b紧密连锁,遗传距离分别为0~3.9 cM和0~0.9 cM。

关 键 词:普通小麦  面粉黄色度  b*值  籽粒黄色素含量  分子标记  QTL分析
收稿时间:2004-12-31
修稿时间:2005-04-02

QTL Mapping for Kernel Yellow Pigment Content in Common Wheat
ZHANG Li-Ping,YAN Jun,XIA Xian-Chun,HE Zhong-Hu,Mark W Sutherland.QTL Mapping for Kernel Yellow Pigment Content in Common Wheat[J].Acta Agronomica Sinica,2006,32(1):41-45.
Authors:ZHANG Li-Ping  YAN Jun  XIA Xian-Chun  HE Zhong-Hu  Mark W Sutherland
Affiliation:Institute of Crop Sciences / National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing 100081
Abstract:Flour yellowness b~* is a major criterion to estimate flour colour,which is basically determined by yellow pigment content in wheat grains.A doubled haploid(DH) population with 71 lines derived from a cross between Zhongyou 9507 with lower flour yellowness b~* and CA9632 with higher flour yellowness b~*,was sown for two years in 5 locations.A total of 173 loci including 122 SSR,4 STS of storage protein genes and 10 AFLP markers were used to construct a linkage map,covering 2 881 cM of whole genome at 21 linkage groups.With the method of composite interval mapping(CIM),8 putative major QTL of yellowness b~* were detected on chromosomes 1DS,2DL,3A,4D,5D,6AL,6D and 7AL(Table 2),and 5 putative QTL of yellow pigment content were detected on chromosome 2DL,3DL,4A,5A and 7AL(Table 3),respectively.The QTL on 7AL was common for yellowness b~* and yellow pigment content,accounting for 12.97.6% and 12.13.9% of the phenotypic variance across different environments,respectively,which was closely linked to Xgwm264b.The genetic distance between Xgwm264b and the QTL of flour yellowness b~* and grain yellow pigment on chromosome 7AL were 03.9 cM and 00.9 cM,respectively.It can be used as a molecular marker in wheat breeding program.
Keywords:Common wheat(Triticum aestivum L  )  Flour yellowness b~*  Kernel yellow pigment  Molecular marker  Quantitative trait loci(QTL)
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