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拟南芥幼苗对高NH4+响应的特征及不同生态型间的差异
引用本文:李保海,施卫明.拟南芥幼苗对高NH4+响应的特征及不同生态型间的差异[J].土壤学报,2007,44(3):508-515.
作者姓名:李保海  施卫明
作者单位:1. 土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,南京,210008;中国科学院研究生院,北京,100049
2. 土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,南京,210008
基金项目:中国科学院知识创新团队国际合作伙伴计划项目;中国科学院知识创新工程项目
摘    要:通过培养基和水培法分析了不同浓度NH+^4处理对苗期拟南芥根系特征的影响,比较了不同生态型拟南芥苗期根形态及生理的差异。结果表明,拟南芥苗期的主根长、侧根长、根表面积,均随NH4^+浓度增高逐渐降低;而根平均直径随NH4^+浓度增高先变粗后变细。不同生态型拟南芥对高浓度NH4^+的响应差异显著,而对等浓度K^+的响应没有观察到与NH4^+的类似的毒害作用及生态型间的差异。应用差异显著的典型生态型(耐NH4^+型Col-0和NH4^+敏感型JA22)进一步研究表明,NH4^+毒害对Col-0根长的抑制作用显著小于JA22,对Col-0平均根直径的促进作用也显著小于JA22,但对二者的根表面积的抑制没有显著性差异;30mmol L^-1 NH4^+处理,Col-0的地上部干重显著高于JA22,叶绿素含量却显著低于JA22,二者的含水量差异不显著;Col-0对2mmol L^-1 NH4^+的净吸收速率是JA22的3倍多。因此高浓度NH4^+抑制苗期拟南芥的生长基本上不是根际酸化、缺NO3^-、高离子强度或对NH4^+的高吸收等因子所致。

关 键 词:拟南芥  铵毒害  根形态  生态型  生理
收稿时间:1/4/2006 12:00:00 AM
修稿时间:2006-01-042006-05-12

EFFECTS OF ELEVATED NH4+ ON ARABIDOPSIS SEEDLINGS DIFFERENT IN ACCESSIONS
Li Baohai and Shi Weiming.EFFECTS OF ELEVATED NH4+ ON ARABIDOPSIS SEEDLINGS DIFFERENT IN ACCESSIONS[J].Acta Pedologica Sinica,2007,44(3):508-515.
Authors:Li Baohai and Shi Weiming
Affiliation:1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2.Graduate School of the Chinese Academy of Sciences, Bering 100049, China
Abstract:The aims of the work were to investigate effect of NH 4 on Arabidopsis seedlings of different accessions growing in agar medium or hydroponics in root characteristics, morphology and physiology. Results indicate primary root length, lateral root length and root surface area of Arabidopsis all decreased with increasing NH 4, but root average diameter went up first and then down with increasing NH 4. Difference between ecotypes of Arabidopsis in response to elevated NH 4 was obvious, but no similar phenomenon was found when the seedlings were exposed to the same concentration of K . Further study on two significantly different accessions of Arabidopsis (Col-0 tolerant to NH 4 and JA22 sensitive to NH 4) show that the effect of NH 4 was significantly lower on Col-0 than on JA22 in inhibiting root length, and so was it in promoting root average diameter, but no much difference was observed between them. Treated with 30 mmol L-1 NH 4, Col-0 was significantly higher than JA22 in biomass of the shoots, but much lower in chlorophyll content, and they are more or less the same in water content. And in terms of NH 4 uptake rate, Col-0 was at least 3 times higher than JA22. Therefore, it could be concluded that the Arabidopsis growth inhibited by NH 4 was not attributed to rhizosphere acidification, absence of NO-3, high ion intensity or high availability of NH 4 to plants.
Keywords:Arabidopsis thaliana  Ammonium toxicity  Root structure  Accessions  Physiology
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